2017
DOI: 10.1016/j.actbio.2017.06.031
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Transcriptome profiling of 3D co-cultured cardiomyocytes and endothelial cells under oxidative stress using a photocrosslinkable hydrogel system

Abstract: Myocardial infarction (MI) is one of the most common among cardiovascular diseases. Endothelial cells (ECs) are considered to have protective effects on cardiomyocytes (CMs) under stress conditions such as MI; however, the paracrine CM-EC crosstalk and the resulting endogenous cellular responses that could contribute to this protective effect are not thoroughly investigated. Here we created biomimetic synthetic tissues containing CMs and human induced pluripotent stem cell (hiPSC)-derived ECs (iECs), which sho… Show more

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Cited by 13 publications
(10 citation statements)
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“…Two other significantly regulated pathways were drug metabolism-cytochrome P450 and metabolism of xenobiotics by cytochrome P450. Xet et al indicated that the activation of the drug metabolism-cytochrome P450 pathway in cardiomyocytes through derived endothelial cell crosstalk could be crucial for cardioprotection under oxidative stress conditions [41]. Cytochrome P450 has been shown to be prevalent in inflammation-related disorders, including cardiovascular disease [42].…”
Section: Discussionmentioning
confidence: 99%
“…Two other significantly regulated pathways were drug metabolism-cytochrome P450 and metabolism of xenobiotics by cytochrome P450. Xet et al indicated that the activation of the drug metabolism-cytochrome P450 pathway in cardiomyocytes through derived endothelial cell crosstalk could be crucial for cardioprotection under oxidative stress conditions [41]. Cytochrome P450 has been shown to be prevalent in inflammation-related disorders, including cardiovascular disease [42].…”
Section: Discussionmentioning
confidence: 99%
“…A recent study reported the transcriptome profiling of 3D co-cultured cardiomyocytes and human ECs under oxidative stress [ 53 ]. In the conditions adopted by the authors, a very limited number of genes was modulated in ECs, possibly due to the stabilizing action of the co-culture with cardiomyocytes.…”
Section: Discussionmentioning
confidence: 99%
“…Throughout the duration of the culture, live and dead cells were stained and counted in order to measure the death rate at the edges, corners and bulk of the cell-laden hydrogels. The hydrogel material, which is arginine-glycine-aspartic acid (RGD) conjugated polyethylene glycol- 4-arm acrylate (PEG–RGD), was chosen to prohibit cells from migrating or proliferating [ 17 , 18 ]. This way we ensured that the observed effects were not due to a loss or gain of physical contact between cells, but due to intercellular cooperation via factors secreted by the cells that diffuse through the hydrogel medium.…”
Section: Resultsmentioning
confidence: 99%