Transcriptome profiling of abiotic responses to cold and drought stress of Cinnamomum kanehirae

Abstract

Stout camphor tree (Cinnamomum kanehirae) is an economically and socially valuable timber species that is conserved in Taiwan Province, China. In this study, Illumina sequencing technology was used to identify genes in C. kanehirae and analyze their levels under drought and low temperature stresses. The obtained reads generated 27,885 single genes, including 8,136 that were differential. Based on the results of Kyoto Encyclopedia of Genes and Genomes enrichment, the primary metabolic pathways in response to drought and low temperature stress included sucrose and starch metabolism, photosynthesis, glycolysis and sugar metabolism synthesis, phenylpropanoid biosynthesis, plant-pathogen interaction, and flavonoid biosynthesis, as well as the identification of transcription factors (TFs) with different patterns of expression. Finally, the RNA-Seq data were validated using real-time fluorescence quantitative analysis to identify TFs with different patterns of expression. These data provide a valuable resource for further research on the molecular mechanisms of drought and low temperature stress in C. kanehirae and contribute to the exploration of drought and cold resistance genes.

Priming avocado with sodium hydrosulfide prior to frost conditions induces the expression of genes involved in protection and stress responses

“…The NAC family encompasses TFs involved in various processes in plants, including growth development and responses to different stresses (Han et al, 2023). NAC family TFs were recently shown to be up-regulated in avocado in response to low temperature (4 C) and drought conditions (Zhang et al, 2023b). MYC2 can bind to the promoters of NAC TFs and activate their expression, with one of these being ANAC72 (Kazan & Manners, 2013), up-regulated in primed trees.…”