2022
DOI: 10.3389/fpls.2022.876428
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Transcriptome Profiling Reveals a Petunia Transcription Factor, PhCOL4, Contributing to Antiviral RNA Silencing

Abstract: RNA silencing is a common antiviral mechanism in eukaryotic organisms. However, the transcriptional regulatory mechanism that controls the RNA silencing process remains elusive. Here, we performed high-depth transcriptome analysis on petunia (Petunia hybrida) leaves infected with tobacco rattle virus (TRV) strain PPK20. A total of 7,402 differentially expressed genes (DEGs) were identified. Of them, some RNA silencing-related transcripts, such as RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzym… Show more

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Cited by 2 publications
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“…In order to study the silencing effect of the VIGS carrier, commonly used marker genes include chloroplastos alterados 1 gene (CLA1), phytoene desaturase (PDS), green fluorescent protein (GFP), and so on [22][23][24]. Among them, CLA1 encodes the 1-deoxy-Dxylulose 5-phosphate synthase, the first enzyme of the 2-C-methyl-d-erythritol-4-phosphate pathway associated with chloroplast development, which is highly conserved in a variety of plants [24].…”
Section: Introductionmentioning
confidence: 99%
“…In order to study the silencing effect of the VIGS carrier, commonly used marker genes include chloroplastos alterados 1 gene (CLA1), phytoene desaturase (PDS), green fluorescent protein (GFP), and so on [22][23][24]. Among them, CLA1 encodes the 1-deoxy-Dxylulose 5-phosphate synthase, the first enzyme of the 2-C-methyl-d-erythritol-4-phosphate pathway associated with chloroplast development, which is highly conserved in a variety of plants [24].…”
Section: Introductionmentioning
confidence: 99%