Transcriptome reveal the response to Cry1Ac toxin in susceptible Bombyx mori

Abstract

Bombyx mori as a representative in Lepidoptera is an important economic insect in agriculture production. Bacillus thuringiensis (Bt) is a bacterial pathogen in silkworm production. Understanding how silkworm respond to Bt‐toxin can provide guidance to cultivate resistant silkworm strains. Cry1Ac is one type of Bt‐toxin. In current research, Dazao, a susceptible B. mori strain to Bt‐toxin, was treated by Cry1Ac toxin and compared its transcriptome with untreated samples. This analysis detected 1234 differentially expressed genes (DEGs). Gene Ontology, KEGG, and UniProt keyword enrichment analysis showed that DEGs include ATP‐binding cassette (ABC) transporter, stress response, cuticle, and protein synthesis, and folding process. Five ABC genes were upregulated after Cry1Ac treatment including ABCA2, ABCA3, and ABCC4. They are also known as the transporters of Bt‐toxin in lepidopteran insect. Expression of cuticle proteins was significantly increased at 6 h after Cry1Ac treatment. Sex‐specific storage‐proteins and heat shock protein were also upregulated in Cry1Ac treated samples. Our data provide an expression profile about the response of Cry1Ac toxin in susceptible B. mori strain.

Profiling of MicroRNAs in Midguts of Plutella xylostella Provides Novel Insights Into the Bacillus thuringiensis Resistance

“…Therefore, the chitin based bioformulation may act as an effective pesticide against Lepidopterous pests. A prior work revealed that the expression of cuticle proteins were siginificantly induced at 6 h posttreatment of Cry1Ac toxin (Qin et al, 2021). Genes encoding cuticle proteins were induced during B. mori bidensovirus (BmBDV) infection (Sun et al, 2020).…”

Protection of HSP60 in Myzus persicae Treated with Cry7Ab4 Toxin

“…However, in contrast to HSP70, the importance of HSP60 in insects is much less understood, particularly in response to Bt toxins. Qin et al found that hsp20 , hsp60 , and hsp70 were upregulated in Bombyx mori (Lepidoptera: Bombycidae) following Cry1Ac toxin treatment. Shabbir et al and Xu et al claimed that the expression level of hsp70 in Cry1Ah- and Cry1Ab-resistant Ostrinia furnacalis (Lepidoptera: Pyralidae) strains was higher than that in Cry1Ah- and Cry1Ab-susceptible strains, and Xu et al identified HSP70 as a putative Cry1Ab-binding protein.…”

Transcriptome analysis ofMythimna separata: De novo assembly and detection of genes related to beta‐cypermethrin resistance

“…For example, transcriptional analysis of M. separata suffering from a podophyllotoxin‐derived insecticidal agent showed that the primary insecticidal mechanism is the wing‐related development pathway (Sun et al, 2011); Genome‐wide transcriptional analysis for two field thiamethoxam‐resistant populations of Diaphorina citri and susceptible psyllids demonstrated that 60s ribosomal proteins, UDP‐gluscoyltransferases, cytochrome c oxidases, CYP, and ABC transporters were upregulated among thiamethoxam‐resistant D. citri (X. D. Chen et al, 2021); a susceptible Bombyx mori strain to Bt‐toxin was treated by Cry1Ac (one type of Bt‐toxin) toxin. Then, the transcriptomes of the treated and the untreated samples were compared, and it was found that five ABC genes, which are known as the transporters of Bt‐toxin in lepidopteran insects, were upregulated in the Cry1Ac treated sample (Qin et al, 2021); The P450s CYP6BB2 , CYP9M9 . and CYP6M11 were found overtranscribed in association with imidacloprid resistance through the combined transcriptomic and genomic data of Aedes aegypti (Zoh et al, 2021).…”