Transcriptome sequencing and analysis of zinc-uptake-related genes in Trichophyton mentagrophytes

Bailão

et al. 2020

JoF

Zinc is an essential nutrient for all living organisms. However, firm regulation must be maintained since micronutrients also can be toxic in high concentrations. This notion is reinforced when we look at mechanisms deployed by our immune system, such as the use of chelators or membrane transporters that capture zinc, when threatened with pathogens, like fungi. Pathogenic fungi, on the other hand, also make use of a variety of transporters and specialized zinc captors to survive these changes. In this review, we sought to explain the mechanisms, grounded in experimental analysis and described to date, utilized by pathogenic fungi to maintain optimal zinc levels.

Section: Zinc Uptake Not Related To Depend On Ph Regulation Thus Fsupporting

confidence: 78%

“…The transcription factor is essential for growth under zinc-limited conditions and is also important for conidiation. Additionally, possible zrfA, zrfB, and zrfC orthologs were also found, but functional analyses are still unavailable [ 100 ].…”

Section: Zinc Uptake Not Related To Depend On Ph Regulation Thus Fmentioning

confidence: 99%

Zinc at the Host–Fungus Interface: How to Uptake the Metal?

Bailão

et al. 2020

JoF

“…With Nr annotation, the Blast2GO program (v2.5.0) with default parameters was used to obtain GO annotation of the unigenes (Conesa et al, 2005). After GO annotation for every unigene, WEGO software (Ye et al, 2006) was used for GO functional classification and graphical representation of GO terms (Zhang et al, 2017). InterProScan5 was used for InterPro annotation (Quevillon et al, 2005).…”

Section: De Novo Transcriptome Assembly and Functional Annotationmentioning

confidence: 99%

Transcriptome analyses of Ditylenchus destructor in responses to cold and desiccation stress

Gao

Wang

et al. 2020

Genet. Mol. Biol.

The objective of this study was to identify molecular responses in Ditylenchus destructor to cold and desiccation by means of transcriptomes analyses. A total of 102,517 unigenes were obtained, with an average length of 1,076 bp, in which 58,453 (57%) had a functional annotation. A total of 1154 simple sequence repeats (SSRs) distributed over 1078 unigenes were detected. Gene expression profiles in response to cold and desiccation stress and the expression of specific stress-related genes were compared. Gene ontology analysis and pathway-based analysis were used to further investigate the functions of the differentially expressed genes. The reliability of the sequencing data was verified through quantitative real-time PCR analysis of 19 stress-related genes. RNA interference used to further assess the functions of the cold-related unigenes 15628 and 15596 showed that the knockdown of each of these genes led to decreased cold tolerance of D. destructor. Hence, this study revealed molecular processes and pathways active in cold-or dessication-treated nematodes. The transcriptome profiles presented in this study provide insight into the transcriptome complexity and will contribute to further understand stress tolerance in D. destructor.

“…The sequencing and annotation of several dermatophyte genomes provide resources that aid in elucidating their mechanisms of virulence. Next‐generation transcriptome sequencing has greatly improved our understanding of the genetic mechanisms of pathogenicity in other dermatophyte species 9,10 . To date, however, a high‐quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive genome and transcriptome analysis of the dermatophyte Trichophyton schoenleinii reveals the candidate pathogenic genes

Liu

Zheng

et al. 2021

Mycoses

Background Trichophyton schoenleinii is an anthropophilic dermatophyte that causes tinea favosa. Nowadays, it remains an important pathogen in some regions of the world, mainly epidemic in Africa and West Asia. Despite the medical importance of T. schoenleinii infections, a high‐quality reference genome for T. schoenleinii is still unavailable, neither its transcriptomic profile. Objectives The aim of the current study was to improve understanding of the underlying pathogenic mechanism of T. schoenleinii, and to define the candidate pathogenic genes of T. schoenleinii. Methods Comprehensive genomic analysis of T. schoenleinii was carried out by Illumina and PacBio sequencing platforms. Transcriptome profiles of T. schoenleinii cultured in vitro in two media containing either keratin or soy protein were determined using RNA sequencing (RNA‐seq) technology. Results Here, we present the first draft genome sequence of T. schoenleinii strain T2s, which consists of 11 scaffolds containing 7474 predicted genes. Transcriptome analysis showed that genes involved in keratin hydrolysis have higher expression in T. schoenleinii grown in keratin medium, including genes encoding proteases, cysteine dioxygenase and acetamidase. Other genes with higher expression include genes encoding the components of the pH‐responsive signal transduction pathways and transcription factors, many of which may play a role in pathogenicity. Conclusion In summary, this study provides new insights into the pathogenic mechanism of T. schoenleinii and highlights candidate genes for further development of novel targets in disease diagnosis and treatment of tinea favosa.