Transcriptome-Wide Identification and Expression Profiling of SPX Domain-Containing Members in Responses to Phosphorus Deprivation of Pinus massoniana

Wang, Conghui; Fan, Fuhua; Shang, Xianwen; Zhou, Zijing; Ding, Guijie

doi:10.3390/f12121627

Cited by 2 publications

(1 citation statement)

References 75 publications

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“…Moreover, SPX1 , SPX3 , and SPX5 were up-regulated under P deficiency and N+P deficiency ( Figure S5 ). Studies have shown that most SPX subfamily members are induced by Pi deficiency in plants ( Stefanovic et al., 2007 ; Zhang et al., 2016 ; Du et al., 2017 ; Wang et al., 2021a ), and their major role is to interact with PHR1, thereby regulating the transcription of downstream PSIs ( Lv et al., 2014 ; Dong et al., 2019 ). Furthermore, PHO1 was down-regulated under three treatments.…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

et al. 2023

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Nitrogen (N) and phosphorus (P) are essential phytomacronutrients, and deficiencies in these two elements limit growth and yield in apple (Malus domestica Borkh.). The rootstock plays a key role in the nutrient uptake and environmental adaptation of apple. The objective of this study was to investigate the effects of N and/or P deficiency on hydroponically-grown dwarfing rootstock ‘M9-T337’ seedlings, particularly the roots, by performing an integrated physiological, transcriptomics-, and metabolomics-based analyses. Compared to N and P sufficiency, N and/or P deficiency inhibited aboveground growth, increased the partitioning of total N and total P in roots, enhanced the total number of tips, length, volume, and surface area of roots, and improved the root-to-shoot ratio. P and/or N deficiency inhibited NO3 − influx into roots, and H+ pumps played a important role in the response to P and/or N deficiency. Conjoint analysis of differentially expressed genes and differentially accumulated metabolites in roots revealed that N and/or P deficiency altered the biosynthesis of cell wall components such as cellulose, hemicellulose, lignin, and pectin. The expression of MdEXPA4 and MdEXLB1, two cell wall expansin genes, were shown to be induced by N and/or P deficiency. Overexpression of MdEXPA4 enhanced root development and improved tolerance to N and/or P deficiency in transgenic Arabidopsis thaliana plants. In addition, overexpression of MdEXLB1 in transgenic Solanum lycopersicum seedlings increased the root surface area and promoted acquisition of N and P, thereby facilitating plant growth and adaptation to N and/or P deficiency. Collectively, these results provided a reference for improving root architecture in dwarfing rootstock and furthering our understanding of integration between N and P signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

et al. 2023

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Genome‑Wide Identification and Expression Analysis of SPX Domain-Containing Gene Subfamily in Response to Phosphorus-Solubilizing Bacteria in Apple (Malus domestica)

Kural,

Kiyak,

Uluisik

et al. 2024

Plant Mol Biol Rep

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Phosphate is one of the major elements that significantly affects fruit yield and quality. The aim of the study was to determine whether using phosphorus-solubilizing bacteria could produce high-quality apple nursery trees. Five different treatments were tested on a “Granny Smith” apple cultivar that was grafted onto an M.9 rootstock. These were 100% P, 50% P, 50% P + Bacillus megatarum (plant growth promoting rhizobacteria, PGPR), 0%P, and 0%P + PGPR. The study also identified the SPX gene family, which is essential for plant growth and development and responds to phosphorus (P) stress. A total of 72 SPX genes were identified in different plant species based on structural and phylogenetic analysis. The apple genome contains seven different SPX genes distributed on five of the 17 chromosomes. Gene structure and motif analysis showed that SPX genes show a relatively conserved exon/intron arrangement and motif composition in five different species: apple, strawberry, peach, apricot, and grape. Protein–protein network analysis showed that SPX proteins are closely related to proteins involved in P metabolism in apple. The digital expression profiles of MdSPX genes among 47 apple tissues were characterized to provide insight into their potential functions. RT-qPCR revealed that the expression level of all MdSPXs was significantly downregulated in 50% P + PGPR treatments, indicating that 50% P combined with PGPR is effectively taken up by the plant, saving it from Pi starvation. These results not only confirm the key role of MdSPXs in Pi homeostasis and the Pi signaling pathway but also clarify the importance of Pi-solubilizing bacteria in plant nutrition.

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Transcriptome-Wide Identification and Expression Profiling of SPX Domain-Containing Members in Responses to Phosphorus Deprivation of Pinus massoniana

Cited by 2 publications

References 75 publications

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions

Genome‑Wide Identification and Expression Analysis of SPX Domain-Containing Gene Subfamily in Response to Phosphorus-Solubilizing Bacteria in Apple (Malus domestica)

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