Transcriptomic Analyses Reveal the Protective Immune Regulation of Conjugated Linoleic Acids in Sheep Ruminal Epithelial Cells

Yang, Chunlei; Lan, Wei; Ye, Shijie; Zhu, Binna; Fu, Zhengwei

doi:10.3389/fphys.2020.588082

Cited by 12 publications

(10 citation statements)

References 65 publications

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“…Nevertheless, we could see a strong upregulation of inflammatory genes TNF-α, IL1B, IL6, and CXCL8 in our study. These results reflect what is seen in other studies with rumen epithelial cells [13,14,36]. Especially for TNF-α, a time-dependent effect could be observed in our study, peaking at 2 h. Again, we want to highlight that based on our results and results of other research groups, the time point after LPS exposure is a critical issue and needs to be considered when performing studies with LPS.…”

Section: Discussionsupporting

confidence: 90%

Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants

Reisinger¹,

Wendner²,

Schauerhuber³

et al. 2021

Animals

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Endotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (REC) were stimulated with LPS and LTA for 1, 2, 4, and 24 h. Thereafter, the expression of selected genes of the LPS and LTA pathway and inflammatory response were evaluated. Furthermore, it was assessed whether LPS affects inflammatory response and structural integrity of claw explants. Therefore, claw explants were incubated with LPS for 4 h to assess the expression of selected genes and for 24 h to evaluate tissue integrity via separation force. LPS strongly affected the expression of genes related to inflammation (NFkB, TNF-α, IL1B, IL6, CXCL8, MMP9) in REC. LTA induced a delayed and weaker inflammatory response than LPS. In claw explants, LPS affected tissue integrity, as there was a concentration-dependent decrease of separation force. Incubation time had a strong effect on inflammatory genes in claw explants. Our data suggest that endotoxins can induce a local inflammatory response in the rumen epithelium. Furthermore, translocation of LPS might negatively impact claw health.

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Section: Discussionsupporting

confidence: 90%

Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants

Reisinger¹,

Wendner²,

Schauerhuber³

et al. 2021

Animals

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show abstract

“…In addition, we found that the differential metabolites in the two groups were mainly concentrated in lipid compounds. Fatty acids can regulate lipid metabolism and immune response in the rumen epithelium [ 61 ]. The aforementioned findings thus support the crucial function of the rumen epithelium’s metabolic adaption to low pH.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Gene Expression Responses of Bovine Rumen Epithelial Cells to Different pH Stresses

Lian

Zhang

Liu

et al. 2022

Animals

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Ruminal acidosis often occurs in production, which greatly affects animal health and production efficiency. Subacute rumen acidosis (SARA) occurs when rumen pH drops rapidly to 5.5–5.8, and acute rumen acidosis (ARA) occurs when rumen pH drops below 5.0, but the molecular regulation mechanism of the rumen epithelium after the rapid decrease in pH is still unclear. Bovine rumen epithelial cells (BRECs) were cultured at pH = 7.4 (control), 5.5 (SARA), and 4.5 (ARA). Transcriptome and metabolomic methods were used to obtain the molecular-based response of BRECs to different pH treatments; pH = 4.5 can significantly induce apoptosis of BRECs. The RNA-seq experiments revealed 1381 differently expressed genes (DEGs) in the control vs. SARA groups (p < 0.05). Fibroblast growth factor (FGF) and tumor necrosis factor (TNF) were upregulated 4.25 and 6.86 fold, respectively, and TLR4 was downregulated 0.58 fold. In addition, 283 DEGs were identified in the control vs. ARA comparison (p < 0.05), and prostaglandin-endoperoxide synthase 2 (PSTG2) was downregulated 0.54 fold. Our research reveals that the MAPK/TNF signaling pathway regulates the inflammatory response of BRECs. Metabolomics identified 35 biochemical compounds that were significantly affected (p < 0.05) in control vs. SARA and 51 in control vs. ARA. Bioinformatics analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database revealed that drug metabolism-cytochrome P450 metabolic and alpha-linolenic acid metabolism changes occurred. These transcriptional and metabolic changes are related to the adaptation of BRECs to low-pH stresses. In conclusion, the combined data analyses presented a worthy strategy to characterize the cellular, transcriptomic, and metabonomic adaptation of BRECs to pH in vitro. We demonstrated transcriptional expression changes in BRECs under pH stress and activation of the molecular mechanisms controlling inflammation.

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“…Despite specific functions of this gene are not known yet, all the aforementioned aspects suggested a possible involving of FAM49A gene in our trait. Similarly, on OAR7, near to SNP rs406841304, two close genes are related with udder conformation ( FAM227B ) ( Scienski et al, 2019 ) and with epithelial cell proliferation and differentiation ( FGF 7) ( Bazer and Slayden, 2008 ; Yang et al, 2020 ), suggesting their possible role in the epithelial mammary cell proliferation. Moreover, the FGF7 has been reported as putative target gene in bovine mammary tissue infected with Streptococcus uberis ( Naeem et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study Identifies New Candidate Markers for Somatic Cells Score in a Local Dairy Sheep

et al. 2021

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In the Mediterranean basin countries, the dairy sheep production is usually based on local breeds, which are very well-adapted to their production systems and environments and can indeed guarantee income, employment, and economic viability in areas where production alternatives are scarce or non-existent. Mastitis is still one of the greatest problems affecting commercial milk production. However, genetic evaluation of mastitis is particularly difficult because of its low heritability and the categorical nature of the trait. The aim of this study was to identify genomic regions putatively associated with somatic cells count (SCC) in the local economically important Valle del Belice sheep breed using of deregressed breeding values (DEBV) as response variables. All the samples were genotyped using the Illumina OvineSNP50K BeadChip. Genome-wide association analysis was carried out based on regression of DEBV. A total of eight markers were found to be significantly associated with log-transformed SCC. Several candidate genes associated with SCC were identified related to immunity system and udder conformation. The results can help improving the competitiveness of the local Valle del Belìce breed. Further studies considering a higher sample size or independent population will be needed to confirm our results.

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Transcriptomic Analyses Reveal the Protective Immune Regulation of Conjugated Linoleic Acids in Sheep Ruminal Epithelial Cells

Cited by 12 publications

References 65 publications

Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants

Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants

In Vitro Gene Expression Responses of Bovine Rumen Epithelial Cells to Different pH Stresses

Genome-Wide Association Study Identifies New Candidate Markers for Somatic Cells Score in a Local Dairy Sheep

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