Transcriptomic Analysis of Gene Networks Regulated by U11 Small Nuclear RNA in Bladder Cancer

Wang, Zhenxing; Wang, Xi; Wang, Yaobang; Tang, Shaomei; Feng, Chao; Pan, Lixin; Lu, Qinchen; Tao, Yuting; Xie, Yuanliang; Wang, Qiuyan; Zhong, Tang

doi:10.3389/fgene.2021.695597

Cited by 3 publications

(4 citation statements)

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“…Wang et al. found that U11 may be involved in the regulation of gene expression in bladder cancer cells, which can alter gene expression by affecting the PI3K-Akt signaling pathway and may be a potential biomarker for the clinical diagnosis and treatment of bladder cancer ( 28 ). In other systems, Cheng et al.…”

Section: Discussionmentioning

confidence: 99%

“…After snoRNAs recognize and bind the complementary sequence on the target rRNA, and they send a signal to the paired protein for accurate base modification to play an important regulatory function under a variety of physiological and pathological conditions ( 24 ). Nuclear small RNA (snRNA) is a kind of small molecular RNA with a length of 100-215 nt in the eukaryotic genome that exists in the nucleus ( 28 ). SnRNA is highly conserved, the content is small in cells, and the expression is spatiotemporally specific ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

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Changes in the Small Noncoding RNAome During M1 and M2 Macrophage Polarization

Zhou

Wang

et al. 2022

Front. Immunol.

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Macrophages belong to a special phagocytic subgroup of human leukocytes and are one of the important cells of the human immune system. Small noncoding RNAs are a group of small RNA molecules that can be transcribed without the ability to encode proteins but could play a specific function in cells. SncRNAs mainly include microRNAs (miRNAs) and piwi-interacting RNAs (piRNAs), small nucleolar RNAs (snoRNAs), small nuclear RNAs (snRNAs) and repeat RNAs. We used high-throughput sequencing analysis and qPCR to detect the expression changes of the small noncoding RNAome during macrophage polarization. Our results showed that 84 miRNAs and 47 miRNAs with were downregulated during M1 macrophage polarization and that 11 miRNAs were upregulated and 19 miRNAs were downregulated during M2 macrophage polarization. MiR-novel-3-nature and miR-27b-5p could promote expression of TNF-α which was marker gene of M1 macrophages. The piRNA analysis results showed that 69 piRNAs were upregulated and 61 piRNAs were downregulated during M1 macrophage polarization and that 3 piRNAs were upregulated and 10 piRNAs were downregulated during M2 macrophage polarization. DQ551351 and DQ551308 could promote the mRNA expression of TNF-α and DQ551351overexpression promoted the antitumor activity of M1 macrophages. SnoRNA results showed that 62 snoRNAs were upregulated and 59 snoRNAs were downregulated during M1 macrophage polarization, whereas 6 snoRNAs were upregulated and 10 snoRNAs were downregulated during M2 macrophage polarization. Overexpression of snoRNA ENSMUST00000158683.2 could inhibit expression of TNF-α. For snRNA, we found that 12 snRNAs were upregulated and 15 snRNAs were downregulated during M1 macrophage polarization and that 2 snRNAs were upregulated during M2 macrophage polarization. ENSMUSG00000096786 could promote expression of IL-1 and iNOS and ENSMUSG00000096786 overexpression promoted the antitumor activity of M1 macrophages. Analysis of repeat RNAs showed that 7 repeat RNAs were upregulated and 9 repeat RNAs were downregulated during M1 macrophage polarization and that 2 repeat RNAs were downregulated during M2 macrophage polarization. We first reported the expression changes of piRNA, snoRNA, snRNA and repeat RNA during macrophage polarization, and preliminarily confirmed that piRNA, snoRNA and snRNA can regulate the function of macrophages.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in the Small Noncoding RNAome During M1 and M2 Macrophage Polarization

Zhou

Wang

et al. 2022

Front. Immunol.

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“…Third, noncoding RNAs have been identified as potential biomarkers for bladder cancer prognosis (165,166). Besides lncRNAs, miRNAs and circRNAs, one study showed that PIWI-interacting RNAs (piRNAs) and snRNAs are important in bladder carcinogenesis (167,168). In this work, it has been shown that 106 piRNAs were increased and 91 piRNAs were decreased in bladder tumor specimens.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 75%

The role of E3 ubiquitin ligases and deubiquitinases in bladder cancer development and immunotherapy

Wang

Zhang

et al. 2023

Front. Immunol.

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Bladder cancer is one of the common malignant urothelial tumors. Post-translational modification (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation, have been revealed to participate in bladder cancer initiation and progression. Ubiquitination is the common PTM, which is conducted by E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme and E3 ubiquitin-protein ligase. E3 ubiquitin ligases play a key role in bladder oncogenesis and progression and drug resistance in bladder cancer. Therefore, in this review, we summarize current knowledge regarding the functions of E3 ubiquitin ligases in bladder cancer development. Moreover, we provide the evidence of E3 ubiquitin ligases in regulation of immunotherapy in bladder cancer. Furthermore, we mention the multiple compounds that target E3 ubiquitin ligases to improve the therapy efficacy of bladder cancer. We hope our review can stimulate researchers and clinicians to investigate whether and how targeting E3 ubiquitin ligases acts a novel strategy for bladder cancer therapy.

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“…Several studies have shown that some snRNP particles regulate migration, invasion and gene regulation in multiple tumor cell lines. 45 , 46 However, the complete understanding of snRNP particles in tumors is still far from satisfactory. RN7SK is a 331 nt snRNA transcribed by RNA polymerase III.…”

Section: Discussionmentioning

confidence: 99%