Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

Kepková, Kateřina Vodičková; Vodička, Petr; Toralova, Tereza; Lopatářová, M.; Čech, Svatopluk; Doležel, R.; Havlíček, V.; Besenfelder, U.; Kuzmany, A.; Sirard, Marc‐André; Laurinčík, Jozef; Kaňka, Jiří

doi:10.1016/j.theriogenology.2010.12.019

Cited by 34 publications

(33 citation statements)

References 52 publications

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“…In addition, in vitro cultured embryos show statistically higher KAT2B expression levels when compared to the in vivo counterparts (Kepkova et al. ). After all these observations, this gene is considered as potential marker for embryo viability at 8‐cell stage.…”

Section: Discussionmentioning

confidence: 99%

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Krania

Dovolou

Rekkas

et al. 2014

Reprod Domestic Animals

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Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue-type plasminogen activator (t-PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus-oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t-PA and/or its inhibitor epsilon-aminocaproic acid (control, t-PA, t-PA+ε-ACA, ε-ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t-PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε-ACA. PAA in the treated group was significantly reduced by ε-ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t-PA-modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t-PA. In conclusion, it appears that excessive t-PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena.

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Section: Discussionmentioning

confidence: 99%

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Krania

Dovolou

Rekkas

et al. 2014

Reprod Domestic Animals

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“…Bovine transcriptome analysis has revealed that part of the decreased reproductive rate in pure breed livestock species might be related to a high degree of inbreeding [65]. Other transcriptomic differences have been reported for IVF blastocysts vs. degenerative embryos [93], in vivo vs. in vitro derived embryos [94], and blastocysts that did or did not result in pregnancy after transfer [95]. In addition, within these analyses, the relative expression Lane et al [85].…”

Section: Assessing Embryo Qualitymentioning

confidence: 90%

“…Interestingly a study using the bovine model reported that preimplantation embryonic development was better in a two-step sequential culture method than in the uninterrupted single media conditions as relative quantitative expression of gene markers related to embryo development showed higher values [102]. A study comparing two commercial culture media reported higher levels of relative mRNA expression in groups cultured in Ménézo B2 media indicating that the embryos were able to develop faster compared to those cultured in COOK media [94].…”

Section: Alternative Culture Approachesmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

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Despite advantages of in vitro embryo production in many species, widespread use of this technology is limited by generally lower developmental competence of in vitro derived embryos compared to in vivo counterparts. Regardless, in vivo or in vitro gametes and embryos face and must adjust to multiple microenvironments especially at preimplantation stages. Moreover, the embryo has to be able to further adapt to environmental cues in utero to result in the birth of live and healthy offspring. Enormous strides have been made in understanding and meeting stage-specific requirements of preimplantation embryos, but interpretation of the data is made difficult due to the complexity of the wide array of culture systems and the remarkable plasticity of developing embryos that seem able to develop under a variety of conditions. Nevertheless, a primary objective remains meeting, as closely as possible, the preimplantation embryo requirements as provided in vivo. In general, oocytes and embryos develop more satisfactorily when cultured in groups. However, optimization of individual culture of oocytes and embryos is an important goal and area of intensive current research for both animal and human clinical application. Successful culture of individual embryos is of primary importance in order to avoid ovarian superstimulation and the associated physiological and psychological disadvantages for patients. This review emphasizes stage specific shifts in embryo metabolism and requirements and research to optimize in vitro embryo culture conditions and supplementation, with a view to optimizing embryo culture in general, and culture of single embryos in particular.

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“…We have recently found that cullin 1 is expressed from two different genes, both of them located on chromosome 4, only in distinct regions [ 26 ]. One of them was identified as Cullin 1 (NM_001193233) and was expressed from the late 8-cell stage to the blastocyst stage.…”

Section: Introductionmentioning

confidence: 99%

Characterization of SCF-Complex during Bovine Preimplantation Development

et al. 2016

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The degradation of maternal proteins is one of the most important events during early development, and it is presumed to be essential for embryonic genome activation (EGA), but the precise mechanism is still not known. It is thought that a large proportion of the degradation of maternal proteins is mediated by the ubiquitin-proteolytic system. In this study we focused on the expression of the Skp1-Cullin1-F-box (SCF) complex, a modular RING-type E3 ubiquitin-ligase, during bovine preimplantation development. The complex consists of three invariable components—Cul1, Skp1, Rbx1 and F-box protein, which determines the substrate specificity. The protein level and mRNA expression of all three invariable members were determined. Cul1 and Skp1 mRNA synthesis was activated at early embryonic stages, at the 4c and early 8c stage, respectively, which suggests that these transcripts are necessary for preparing the embryo for EGA. CUL1 protein level increased from MII to the morula stage, with a significant difference between MII and L8c, and between MII and the morula. The CUL1 protein was localized primarily to nuclei and to a lesser extent to the cytoplasm, with a lower signal in the inner cell mass (ICM) compared to the trophectoderm (TE) at the blastocyst stage. The level of SKP1 protein significantly increased from MII oocytes to 4c embryos, but then significantly decreased again. The localization of the SKP1 protein was analysed throughout the cell and similarly to CUL1 at the blastocyst stage, the staining was less intensive in the ICM. There were no statistical differences in RBX1 protein level and localization. The active SCF-complex, which is determined by the interaction of Cul1 and Skp1, was found throughout the whole embryo during preimplantation development, but there was a difference at the blastocyst stage, which exhibits a much stronger signal in the TE than in the ICM. These results suggest that all these genes could play an important role during preimplantation development. This paper reveals comprehensive expression profile, the basic but important knowledge necessary for further studying.

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Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition

Cited by 34 publications

References 52 publications

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Characterization of SCF-Complex during Bovine Preimplantation Development

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