Transcriptomic analysis of juvenile Chinese sea bass (Lateolabrax maculatus) anesthetized by MS-222 (tricaine methanesulfonate) and eugenol

Dong, Hongbiao; Wang, Wenhao; Duan, Yafei; Li, Hua; Liu, Qingsong; Sun, Yue; Zhang, Jiasong

doi:10.1007/s10695-019-00755-x

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…Anesthetic is useful for reducing stress and improving sh welfare during handling. However, only MS-222 and eugenol have been studied, and they could cause damage to the liver and gills of L. maculatus (Wang et al, 2018;Wang et al, 2020;Dong et al, 2020a). Therefore, more anesthetics need to be tested on this species.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, no report conducts on the anesthetic effect of essential oil on L. maculatus. However, some previous studies have shown that eugenol is more effective and safer than MS-222 (Wang et al, 2018;Wang et al, 2020;Dong et al, 2020a;Cao et al, 2019). Therefore, our study aimed to compare the anesthetic effects, blood parameters and gill physiological changes after exposure to the essential oil of M. denudate and eugenol.…”

Section: Introductionmentioning

confidence: 97%

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Essential oil of Magnolia denudata is an effective anesthetic for spotted seabass (Lateolabrax maculatus): a test of its effect on blood biochemistry, physiology, and gill morphology

Zeng

Dong

et al. 2022

Fish Physiol Biochem

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Magnolia denudata is a well-known ornamental tree in China due to its beautiful blossoms, and it has been used as an analgesic to treat human headaches. This study investigates the anesthetic potential and physiological response of the essential oils of M. denudata ower on spotted seabass Lateolabrax maculatus. Fish (mean ± SD, 164.16 ± 15.40 g) were individually exposed to different concentrations of

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Essential oil of Magnolia denudata is an effective anesthetic for spotted seabass (Lateolabrax maculatus): a test of its effect on blood biochemistry, physiology, and gill morphology

Zeng

Dong

et al. 2022

Fish Physiol Biochem

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“…30 In this respect, insufficient branchial ventilation is a well-documented undesirable side effect of euthanasia. 31 Furthermore, Dong et al 32 reported that exposure to MS-222 and eugenol…”

Section: Discussionmentioning

confidence: 99%

“…31 Furthermore, Dong et al. 32 reported that exposure to MS-222 and eugenol regulated the metabolic pathways and triggered an immunoreaction in the gills of Asian sea bass ( Lateolabrax maculatus ). In their study, Wang et al.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

et al. 2023

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In experimental procedures inevitably leading to the sacrifice of animals, suitable measures should be taken to minimise their pain and suffering as much as possible, as well as to prevent any modification or masking in the experimental results obtained. An overdose of anaesthetic is the method of euthanasia most employed in fish, since it is effective and easy to apply. Our objectives were to compare the efficacy of eugenol and of tricaine methanesulfonate (MS-222) as euthanasia agents in zebrafish, and to make a histological evaluation of the possible effects derived from their application. The concentrations established for eugenol were 0.25 and 0.35 mg/mL, and those for MS-222 were 0.25 and 0.50 mg/mL, for both the buffered solution and the non-buffered one. Eugenol turned out to be a stronger euthanasia agent than MS-222 in zebrafish, presenting with significantly shorter euthanasia times. However, the exposure of the fish to euthanasia doses of eugenol triggered branchial alterations, in addition to serious lesions and changes in their nerve tissue. The results obtained with MS-222 also revealed a marked branchial alteration derived from its use. In this respect, the addition of a buffer to the MS-222 solution enhanced the effectiveness of the drug, with significantly shorter euthanasia times being achieved than with the non-buffered solution, and diminished the severity of the lesions described. We therefore determined that the buffered MS-222 solution is the most effective, reliable and safest method of euthanasia for use in research on zebrafish.

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“…After a 4-month chronic exposure experiment, all fish were anesthetized with 0.03% tricaine (MS-222; Sigma, United States) ( Dong et al, 2020 ). The body length and body weight of each individual in each group were measured and recorded ( n = 60 per group).…”

Section: Methodsmentioning

confidence: 99%

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

et al. 2021

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The ever-increasing production and processing of textiles will lead to greater risks of releasing pollutants into the environment. Textile wastewater treatment plants (TWTPs) effluent are an important source of persistent toxic pollutants in receiving water bodies. The effects of specific pollutants on organisms are usually studied under laboratory conditions, and therefore, comprehensive results are not obtained regarding the chronic combined effects of pollutants under aquatic environmental conditions. Thus, this study aimed to determine the combined effects of TWTP effluents on the growth performance, oxidative stress, inflammatory response, and intestinal microbiota of adult zebrafish (Danio rerio). Exposure to TWTP effluents significantly inhibited growth, exacerbated the condition factor, and increased the mortality of adult zebrafish. Moreover, markedly decreases were observed in the activities of antioxidant enzymes, such as CAT, GSH, GSH-Px, MDA, SOD, and T-AOC, mostly in the intestine and muscle tissues of zebrafish after 1 and 4 months of exposure. In addition, the results demonstrated that TWTP effluent exposure affected the intestinal microbial community composition and decreased community diversity. Slight changes were found in the relative abundance of probiotic Lactobacillus, Akkermansia, and Lactococcus in zebrafish guts after chronic TWTP effluent exposure. The chronic toxic effects of slight increases in opportunistic pathogens, such as Mycoplasma, Stenotrophomonas, and Vibrio, deserve further attention. Our results reveal that TWTP effluent exposure poses potential health risks to aquatic organisms through growth inhibition, oxidative stress impairment of the intestine and muscles, and intestinal microbial community alterations.

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Transcriptomic analysis of juvenile Chinese sea bass (Lateolabrax maculatus) anesthetized by MS-222 (tricaine methanesulfonate) and eugenol

Cited by 9 publications

References 38 publications

Essential oil of Magnolia denudata is an effective anesthetic for spotted seabass (Lateolabrax maculatus): a test of its effect on blood biochemistry, physiology, and gill morphology

Essential oil of Magnolia denudata is an effective anesthetic for spotted seabass (Lateolabrax maculatus): a test of its effect on blood biochemistry, physiology, and gill morphology

Comparative study of tricaine methanesulfonate (MS-222) and eugenol as euthanasia agents in zebrafish (Danio rerio) as an experimental model

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

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