Transcriptomic analysis of mechanism underlying the effect of induced molting on semen quality and reproductive performance in aged Houdan roosters

Zhu, Tong; Liang, Wenjie; He, Yuehua; Zhang, Binbin; Liu, Cong; Wang, Dongxue; Deng, Lekun; Li, Donghua; Li, Wenting; Yan, Fengbin; Tian, Yadong; Han, Ruili; Kang, Xin; Li, Zhuanjian; Jiang, Ruirui; Sun, Guirong

doi:10.1016/j.psj.2023.102935

Cited by 4 publications

(5 citation statements)

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“…Meanwhile, the testis is an important parameter for evaluating male reproductive capacity [ 32 ]. From our results, it can be perceived that the testis weight and semen quality of D Group roosters have been improved, which is consistent with previous research results [ 1 ]. In this study, 61 DEGs were identified by transcriptome sequencing of testis tissues from 350-day-old Houdan roosters, which underwent induced molting and recovery compared to those that did not undergo induced molting and recovery.…”

Section: Discussionsupporting

confidence: 92%

“…There is evidence to suggest a correlation between testis weight and semen quality capacity [ 1 , 6 ]. Meanwhile, the testis is an important parameter for evaluating male reproductive capacity [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…It was discovered by researchers Zhu et al that the sperm motility and reproductive capacity of roosters were enhanced after induced molting. Through transcriptome sequencing analysis of rooster testicles, it was found that the regulation of cellular cytoskeleton is closely associated with spermatogenesis [ 1 ]. Cytokines play an important role in establishing and maintaining the immune characteristics of the testis [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

“…The reproductive performance of Breeder roosters plays a crucial role in poultry production. Breeder roosters experience a significant decline in semen quality as they age, putting them at risk of being culled in the subsequent years [ 1 ]. The fertility of roosters typically peaks at 12 months and then rapidly declines [ 2 – 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have reported that light fasting or intermittent fasting was beneficial to the health of humans and animals, enhancing systemic immune function and slowing down aging [ 30 , 31 ]. In chickens, study found that molting can stimulate testis growth and development in roosters [ 1 ]. However, the underlying molecular mechanism associated with the fasting-induced molting in roosters remains unclear, necessitating further investigation into the genetic networks responsible for this phenotype.…”

Section: Introductionmentioning

confidence: 99%

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Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis

Liang,

He,

Zhu

et al. 2024

BMC Genomics

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Background The breeder rooster has played a pivotal role in poultry production by providing high-quality semen. Typically, fertility peaks between 30 and 40 weeks of age and then declines rapidly from 45 to 55 weeks of age. Research into improving fertility in aging roosters is essential to extend their productive life. While progress has been made, enhancing fertility in aging roosters remains a significant challenge. Methods To identify the genes related to promoting sperm remodeling in aged Houdan roosters, we combined changes in testis and semen quality with transcriptome sequencing (RNA-seq) to analyze the synchrony of semen quality and testis development. In this study, 350-day-old Houdan breeder roosters were selected for RNA-seq analysis in testis tissues from induced molting roosters (D group) and non-induced molting roosters (47DG group). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected six DEGs to verify the accuracy of the sequencing by qPCR. Results Compared with the 47DG group, sperm motility (P < 0.05), sperm density (P < 0.01), and testis weight (P < 0.05) were significantly increased in roosters in the D group. Further RNA-seq analysis of the testis between the D group and 47DG group identified 61 DEGs, with 21 up-regulated and 40 down-regulated. Functional enrichment analysis showed that the DEGs were primarily enriched in the cytokine-cytokine receptor interaction, Wnt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, and focal adhesion pathway. The qRT-PCR results showed that the expression trend of these genes was consistent with the sequencing results. WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, and SLC26A7 may play a role in testis development and spermatogenesis. This study provides fundamental data to enhance the reproductive value of aging roosters.

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Section: Discussionsupporting

confidence: 92%