Transcriptomic analysis of Raphidocelis subcapitata exposed to erythromycin: The role of DNA replication in hormesis and growth inhibition

Guo, Jiahua; Ma, Zhihua; Jie, Peng; Mo, Jiezhang; Li, Qi; Guo, Jipu; Yang, Fuwei

doi:10.1016/j.jhazmat.2020.123512

Cited by 60 publications

(24 citation statements)

References 66 publications

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“…This may lead to higher toxicity by prolonging dwell time and the effect of LIN in R. subcapitata [49,50]. In accordance with this, a reduction of ABC transporter family genes was detected in both 80 (ABC transporter family A, G) and 120 µg L −1 ERY-dosing groups (ABC transporter family A, B, I, G) [10].…”

Section: Genes Related To Xenobiotic Metabolismsupporting

confidence: 63%

“…Downregulation of cyt b5 may thus reduce the activity of cyt p450, which converts the lipophilic xenobiotics to more water-soluble compounds by facilitating the excretion of certain compounds [42]. In line with this, ERY, which has the same characteristics as LIN, inhibited the cytochrome p450 activity in R. subcapitata [10]. The amidase family, which is involved in mycothiol-mediated detoxification, can be used to regenerate mycothiol, which is a common protective agent under environmental stress conditions and it mainly serves to improve cell robustness and survival [43].…”

Section: Genes Related To Xenobiotic Metabolismmentioning

confidence: 84%

“…However, the majority of the ecotoxicity data are confined to EC50 levels on account of algal growth. Besides, the realistic exposure level of chemicals was far below the acute toxicity test concentrations, approximately 1-6 orders of magnitude lower [10]. Therefore, it is crucial to move to chronic testing using environmentally relevant concentrations, focusing on alteration at the genomic level [11].…”

Section: Introductionmentioning

confidence: 99%

“…The transcriptomic analysis by next-generation sequence (NGS) can facilitate the identification of molecular-level changes that underlie responses to chemical stressors [20]. This technique has been used to detected algal responses to environmental pollutants (e.g., nonylphenol and erythromycin) [10,21]. For instance, transcriptome analysis demonstrated that carbon fixation in photosynthesis, photosynthesis, fatty acid synthesis, and other pathways were changed at 0.09 mg L −1 ROX dosing groups in R. subcapitata [9].…”

Section: Introductionmentioning

confidence: 99%

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Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

et al. 2020

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Lincomycin (LIN), as a waterborne contaminant, may pose a threat to algal health and may affect the provision of ecosystem services. In addition, the molecular mechanisms of lincomycin in algae are still unknown. Here, we attempted to use the transcriptome analysis to elucidate for the first time the potential impact of LIN at an environmentally relevant concentration on the algal growth, and verify the hypothesis that lincomycin can disrupt algal protein synthesis by combining with its subunits of ribosome at high-LIN level. In this study, 7-day growth inhibition tests and RNA-seq sequencing were conducted in Raphidocelis subcapitata (R. subcapitata) in response to a LIN at the concentrations of 0.5 µg L−1 (low), 5 µg L−1 (medium), and 400 µg L−1 (high) treatment groups. A negligible influence on algal growth and merely 21 (21 up- and 0 downregulated) differentially expressed genes (DEGs) was observed at low concentration of LIN, and medium groups showed a 13.4% inhibition and 92 (64 up- and 48 downregulated) DEGs, while high-LIN dosing caused 65.4% reduction in algal growth and 2514 (663 up- and 1851 downregulated) DEGs. In 0.5 and 5 µg L−1 groups, LIN upregulated the genes in the process of photosynthesis consisting of photosynthesis-antenna proteins, and porphyrin and chlorophyll metabolism pathways, suggesting that photosynthesis at low LIN exposure was more sensitive than algal growth. Whereas DEGs in the 400 µg L−1 group were mostly enriched in carbohydrate, carbon fixation in photosynthetic organisms, and nucleotide metabolism pathways. Furthermore, genes involved in detoxification processes were nearly downregulated in high-LIN group. In addition, genes encoding the antioxidant enzymes in the peroxisome pathway such as superoxide dismutase (sod2), peroxin-2 (pex2), 2,4-dienoyl-CoA reductase ((3E)-enoyl-CoA-producing) (decr2) were upregulated, which are responsible for deleting extra intracellular reactive oxygen species (ROS) caused by LIN to protect algal health, suggesting the occurrence of oxidative stress. Taken together, this is the first meticulous study unraveling the molecular mechanism of antibiotics in algae.

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Section: Genes Related To Xenobiotic Metabolismsupporting

confidence: 63%

Section: Genes Related To Xenobiotic Metabolismmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

et al. 2020

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“…The ROS would promote the change of cell permeability, which accelerate the release of intracellular substances (Jiang et al 2021). The increase in membrane permeability is one of the fundamental reasons for increasing the transfer efficiency of ARGs (Guo et al 2021, Lu et al 2020b, Sun et al 2018. And then, to make matters worse, the graphene oxide (GO) would inevitably be released into the aquatic environment with its extensive application (Yang et al 2019, Zhu et al 2019.…”

Section: Introductionmentioning

confidence: 99%

Mutual impacts and interactions of antibiotic resistance genes, microcystin synthetase genes, graphene oxide, and Microcystis aeruginosa in synthetic wastewater

et al. 2021

Environ Sci Pollut Res

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The physiological impacts and interactions of ARGs abundance, microcystin synthetase genes expression, GO, and M. aeruginosa in synthetic wastewater were investigated. The results demonstrated that the absolute abundance of sul1, sul2, tetW, and tetM in synthetic wastewater dramatically increased to 365.2%, 427.1%, 375.2%, and 231.7%, respectively, when the GO concentration was 0.01 mg/L. Even more interesting is that the sum gene copy numbers of mcyA-J also increased to 243.2%. The appearance of GO made the significant correlation exist between ARGs abundance and mcyA-J expression. Furthermore, M. aeruginosa displayed better photosynthetic performance and more MCs production at 0.01 mg/L GO. There were 65 pairs of positive correlations between the intracellular differential metabolites of M. aeruginosa and the abundance of sul1, sul2, tetM, and tetW with various GO concentrations. The GO will impact the metabolites and metabolic pathway in M. aeruginosa. The metabolic changes impacted the ARGs, microcystin synthetase genes, and physiological characters in algal cells. Furthermore, there were complex correlations among sul1, sul2, tetM, tetW, mcyA-J, MCs, photosynthetic performance parameters, and ROS. The different concentration of GO will aggravate the hazards of M. aeruginosa by promoting the expression of mcyA-J, producing more MCs, simultaneously, it may cause the spread of ARGs.

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Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

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BACKGROUND: Pesticides are extensively applied globally. Pesticide residues induce calamitous effects on the environment and untargeted organisms. Public concerns for the safety of freshwater organisms and the challenges posed by aquatic contaminants remain high. In the present study, the acute toxicity of avermectins (AVMs) to the crayfish, Procambarus clarkii was evaluated. We also evaluated the potential effects of AVM on the biochemical and transcriptomic status of the hepatopancreas and gastrointestinal tract in P. clarkii. RESULTS:The 24, 48, 72, 96 h median lethal concentrations (LC 50 ) of AVM on crayfish were 2.626, 1.162, 0.723, 0.566 mg L −1 , respectively. The crayfish were then exposed to 0.65 mg L −1 of AVM for 96 h. AVM significantly altered biochemical parameters including AChE and CAT activities in the hepatopancreas, and AChE, SOD and Na + -K + -ATPase activities in the gastrointestinal tract at several time points. Furthermore, transcriptomic analysis identified 953 and 1851 differentially-expressed genes (DEGs) in the hepatopancreas and gastrointestinal tract, respectively. KEGG enrichment showed that the gene expression profiles of the hepatopancreas and gastrointestinal tract were distinct from each other. The DEGs in the hepatopancreas were mostly enriched with stress-response pathways, while the majority of the DEGs in the gastrointestinal tract belonged to metabolism-related pathways.CONCLUSION: We demonstrated that the AVM induced acute toxicity, oxidative stress, osmoregulation disturbance, neurotoxicity and transcriptome imbalance in crayfish. These findings unraveled the detrimental effects of AVMs exposure on crayfish.

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Transcriptomic analysis of Raphidocelis subcapitata exposed to erythromycin: The role of DNA replication in hormesis and growth inhibition

Cited by 60 publications

References 66 publications

Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

Lincomycin-Induced Transcriptional Alterations in the Green Alga Raphidocelis subcapitata

Mutual impacts and interactions of antibiotic resistance genes, microcystin synthetase genes, graphene oxide, and Microcystis aeruginosa in synthetic wastewater

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

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