Transcriptomic analysis of the porcine endometrium during early pregnancy and the estrous cycle

Franczak, Anita; Wojciechowicz, Bartosz; Kotwica, Genowefa

doi:10.1016/j.repbio.2013.07.001

Cited by 57 publications

(56 citation statements)

References 46 publications

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“…On Day 14 of pregnancy, downregulation of genes encoding enzymes involved in the generation and release of prostaglandin precursors, such as polyunsaturated fatty acids and arachidonic acid, occurs (59). Furthermore, expression of hydroxyprostaglandin dehydrogenase 15-(NAD) (HPGD) mRNA is downregulated (tenfold) on Day 12 of pregnancy, increases on Day 14, and is upregulated on Days 15-16 of pregnancy (60). Together, these findings suggest upregulation of PGF 2 a and PGE2 production and release into the uterine lumen during the phase of initial conceptus elongation and MRP, confirming the mechanistic model of the change to exocrine PGF 2 a release at the molecular level.…”

Section: Transcriptome Studies Of Porcine Endometrium During the Preimentioning

confidence: 99%

Uterine Responses to the Preattachment Embryo in Domestic Ungulates: Recognition of Pregnancy and Preparation for Implantation

Bauersachs

Wolf²

2015

Annu. Rev. Anim. Biosci.

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The endometrium is a tissue newly evolved with the development of mammalian species. Its main function is the support of embryonic growth and development and the nutrition of the fetus. The species-specific differences in establishment and maintenance of pregnancy make the study of this tissue in various mammalian organisms particularly interesting. With the application of omics technologies to various mammalian species, many systematic studies of endometrial gene expression changes during the phase of establishment of pregnancy have been performed to obtain a global view of regulatory events associated with this biological process. This review summarizes the results of trancriptome studies of bovine, porcine, and equine endometrium. Furthermore, the results are compared between these species and to humans. Because an increasing number of studies suggest an important role of small regulatory RNAs (i.e., microRNAs), recent findings related to the regulation of endometrial functions and the development of the conceptus are presented.

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Section: Transcriptome Studies Of Porcine Endometrium During the Preimentioning

confidence: 99%

Uterine Responses to the Preattachment Embryo in Domestic Ungulates: Recognition of Pregnancy and Preparation for Implantation

Bauersachs

Wolf²

2015

Annu. Rev. Anim. Biosci.

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“…We expect that these differences in gene expression patterns to help identify candidate genes that contribute to differences in litter size. There have been some expression studies carried out in pigs looking at changes in endometria [14][15][16][17][18], ovaries [3], and placentas [19], and genes differently expressed at one stage of pregnancy have been identified. Furthermore, important biological processes and pathways were identified.…”

Section: Introductionmentioning

confidence: 99%

Differential Gene Expression in Uterine Endometrium During Implantation in Pigs1

Xiao

Chen

et al. 2015

Biology of Reproduction

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Embryonic mortality during the implantation period strongly affects litter size in pigs. To analyze the differentially expressed genes (DEGs) in the endometrium during implantation and further to identify candidate genes for litter size, tissues of endometrial attachment sites and intersites were collected from nine pregnant sows on Days 13, 18, and 24 of pregnancy. Endometrium tissue was also collected from another three nonpregnant sows. Samples were hybridized to the porcine Agilent GeneChip microarray. The analysis of gene expression patterns over the implantation period revealed 858 DEGs at endometrial attachment sites. Comparisons of the gene files of attachment sites and intersites revealed 12, 51, and 89 DEGs on Days 13, 18, and 24 of pregnancy, respectively. Annotated function was used to identify overrepresented genetic processes, and several biological processes were considered as the most enriched. Genes related to vascular development, proteolysis, RNA metabolism and translation, protein modification, immune response, and hormone-related are discussed in detail. Then we combined microarray technology and linkage analysis to select powerful candidate genes for quantitative trait loci affecting pig litter size. Eighty-seven DEGs were located in quantitative trait loci related to litter size, that is, total number born and number born alive. Those candidate genes were thought to affect litter size by influencing embryonic implantation. Furthermore, single nucleotide polymorphism of VEGFA was shown to be associated with litter size in pigs. This study identified candidate genes for litter size that were related to embryonic implantation and could be a resource for target studies of genetic markers for litter size in pigs.

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“…In recent years, high-throughput sequencing have been performed using animal reproductive tissues, such as endometrium 20 , ovaries 21 , placenta 33 , follicles 34 and granulosa cells 35 , to identify the genes and genetic loci that affect litter size. Compared to litter size, the studies on the molecular genetic mechanism of estrus expression have seldom been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies found that differentially expressed genes (DEGs)), were related to follicular development and hormone metabolism 20–22 .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts

Chu

Zhou

et al. 2017

Sci Rep

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Estrus expression by gilts and sows is hereditable and important for heat detection. To better understand the molecular biological mechanisms of estrus expression in gilts, the mRNA expression profiles of follicular tissue from Large White gilts in diestrus (LD, n = 3) and estrus (LE, n = 3), and Chinese indigenous Mi gilts in diestrus (MD, n = 2) and estrus (ME, n = 3) were investigated using RNA sequencing. We detected 122,804-335,295 SNPs, 6,140-14,947 InDel and 12 types of AS events (39.57% TSS, 34.90% TTS) in 11 samples. A total of 2,838 differentially expressed genes (DEGs) were found in LD vs MD, LE vs ME, LE vs LD, or ME vs MD comparisons. Two DEGs (ACP5 and PIGS) were observed in all comparisons. Two new genes (ENSSSCG00000028235 and ENSSSCG00000021903) were exclusively expressed in Mi and Large White gilts, respectively. Bioinformatics analyses indicate that these DEGs are involved in single-organism process, catalytic activity, cell adhesion and enriched in ECM-receptor interaction, olfactory transduction, ovarian steroidogenesis, steroid biosynthesis and CAMs signaling pathways. These results of RNA-Seq have provided important information for screening the key functional genes or molecular markers of estrus expression in gilts.

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Transcriptomic analysis of the porcine endometrium during early pregnancy and the estrous cycle

Cited by 57 publications

References 46 publications

Uterine Responses to the Preattachment Embryo in Domestic Ungulates: Recognition of Pregnancy and Preparation for Implantation

Uterine Responses to the Preattachment Embryo in Domestic Ungulates: Recognition of Pregnancy and Preparation for Implantation

Differential Gene Expression in Uterine Endometrium During Implantation in Pigs1

Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts

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