Transcriptomic Analysis of Xylan Oligosaccharide Utilization Systems in Pediococcus acidilactici Strain BCC-1

Abstract

Xylan oligosaccharides (XOS) are the hydrolysates of xylan. To compare the proliferation effect of XOS, glucose, fructo oligosaccharides (FOS), xylose, XOS, and a media without carbohydrate source (control) on Pediococcus acidilactici strain BCC-1, the de novo sequencing of Pediococcus acidilactici strain BCC-1 was conducted, and the underlying mechanism of prebiotic xylo oligosaccharide between xylose and XOS was revealed through transcriptomic analysis. Compared to FOS, glucose, and xylose, XOS exhibits a good performance in promoting the fermentation of Pediococcus acidilactici BCC-1. The genome of Pediococcus acidilactici BCC-1 revealed genes encoding XOS transportation and utilized related enzymes, including ATP-binding cassette (ABC) transporters, arabinofuranosidase, xylanase, xylosidase, xylose isomerase, and xylulose kinase. Transcriptome analysis showed that XOS treatment enhanced genes involving carbohydrate metabolism, an ABC transporter sugar system, pentose and glucuronate interconversions, pyruvate metabolism, and the TCA process when compared to xylose treatment. It suggested XOS treatment enhanced sugar absorption and utilization. These results are useful in the understanding of the metabolic pathway of XOS in Pediococcus acidilactici BBC-1 and may contribute to the optimization of the probiotic effect of Pediococcus acidilactici BCC-1 as novel complex feed additives.

Metabolome and Microbiota Analysis Reveals the Conducive Effect of Pediococcus acidilactici BCC-1 and Xylan Oligosaccharides on Broiler Chickens

“…Therefore, decreased concentrations of 3hydroxy-3-methylglutaric acid and succinate tend to prevent inflammation and maintain the gut health of broilers. Our previous study has been demonstrated P. acidilactici BCC-1 can effectively use xylose as an energy source (Lei et al, 2018) and other study found some Pediococcus acidilactici strains could utilize D-arabitol (Wang et al, 2019). Also, some Pediococcus species such as Pediococcus parvulus can effectively use sorbitol (Pérez-Ramos et al, 2017).…”

“…XOS (extracted from corncob, 95% purity, DP of 2-7 and containing 95.6% XOS, 2.4% xylose, 1.4% glucose, 0.5% arabinose, and 0.1% raffinose) was obtained from Shandong LongLive Biotechnology (DeZhou, Shandong, China). P. acidilactici BCC-1 was isolated from the cecum of 36-day-old broiler without feeding any antibiotics (Lei et al, 2018).…”

“…It has been demonstrated through in vitro studies that XOS could promote the proliferation of P. acidilactici BCC-1, which harbors genes with carbohydrate transport and metabolism functions (Lei et al, 2018). However, the mechanism through which P. acidilactici BCC-1 improves growth performance and gut health is yet to be elucidated.…”

Bacteriocinogenic lactic acid bacteria in the traditional cereal-based beverage Boza: a genomic and functional approach

“…Antibiotic resistance, prophages, plasmid and plasmid-associated bacteriocins have been indicated as source of variability at P. pentosaceus genomes 9 . Our P. acidilactici strain, however, also contains a plasmidassociated bacteriocin operon (pediocin PA-1), differing from other strains reported in the literature, such as ZPA017 and BCC-1 10,11 . The metabolic characteristics of the strains obtained indicated a wider metabolic potential for carbohydrate transport for the P. acidilactii transport while the P. pentosaceus presented he capability to synthesize lysine and had quorum sensing related genes.…”

“…Based on the pangenomes of our isolates against reference genomes, we propose P. pentosaceus ST31BZ as a new strain of P. acidilactici and strains ST75BZ and ST87BZ as a new strain of P. pentosaceus. The two closest strains to P. acidilactici were ZPA017 and BCC-1, isolated from feces of a healthy pig 10 and cecum of a broiler chicken 11 , respectively. The unique genes present in our isolates were related to antibiotic resistance and prophages, such as glycopeptide antibiotics resistance protein (COG4767) and phage portal protein BeeE (COG4695) in P. acidilactici ST31BZ (Supplementary Table 3); and ABC-type multidrug transport system (COG0842) and phage terminase large subunit (COG1783) in P. pentosaceus ST75BZ and ST87BZ.…”