Transcriptomic analysis reveal the responses of dendritic cells to VDBP

Cao, Biwei; Wen, Tao; Wang, Meng; Xiong, Yuan; Wan, Lei; Zhu, Li; Zhou, Jing

doi:10.1007/s13258-022-01234-z

Cited by 2 publications

(2 citation statements)

References 71 publications

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“…However, the higher rates of hypovitaminosis D in individuals with DS and autoimmune disorders could provide a potential therapeutic target for this population to either prevent or adjunctly treat autoimmune disorders. Vitamin D is crucial for immune regulation as its receptors are expressed in various immune cells, including T-cells and dendritic cells [ 32 – 35 ]. Vitamin D assists in modulating T-cell proliferation and promoting anti-inflammatory responses in the brain [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Hypovitaminosis D in persons with Down syndrome and autism spectrum disorder

Boyd,

Nguyen,

Khoshnood

et al. 2023

J Neurodevelop Disord

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Background Plasma levels of vitamin D have been reported to be low in persons with Down syndrome (DS) and existing data is limited to small and homogenous cohorts. This is of particular importance in persons with DS given the high rates of autoimmune disease in this population and the known relationship between vitamin D and immune function. This study sought to investigate vitamin D status in a multi-center cohort of individuals with DS and compare them to individuals with autism spectrum disorder (ASD) and neurotypical (NT) controls. Methods A retrospective, multi-center review was performed. The three sites were located at latitudes of 42.361145, 37.44466, and 34.05349. Patients were identified by the International Classification of Diseases (ICD)-9 or ICD-10 codes for DS, ASD, or well-child check visits for NT individuals. The first vitamin D 25-OH level recorded in the electronic medical record (EMR) was used in this study as it was felt to be the most reflective of a natural and non-supplemented state. Vitamin D 25-OH levels below 30 ng/mL were considered deficient. Results In total, 1624 individuals with DS, 5208 with ASD, and 30,775 NT controls were identified. Individuals with DS had the lowest mean level of vitamin D 25-OH at 20.67 ng/mL, compared to those with ASD (23.48 ng/mL) and NT controls (29.20 ng/mL) (p < 0.001, 95% CI: −8.97 to −6.44). A total of 399 (24.6%) individuals with DS were considered vitamin D deficient compared to 1472 (28.3%) with ASD and 12,397 (40.3%) NT controls (p < 0.001, 95% CI: −5.43 to −2.36). Individuals with DS with higher body mass index (BMI) were found to be more likely to have lower levels of vitamin D (p < 0.001, 95% CI: −0.3849 to −0.1509). Additionally, having both DS and a neurologic diagnosis increased the likelihood of having lower vitamin D levels (p < 0.001, 95% CI: −5.02 to −1.28). Individuals with DS and autoimmune disease were much more likely to have lower vitamin D levels (p < 0.001, 95% CI: −6.22 to −1.55). Similarly, a history of autoimmunity in a first-degree relative also increased the likelihood of having lower levels of vitamin D in persons with DS (p = 0.01, 95% CI: −2.45 to −0.63). Conclusions Individuals with DS were noted to have hypovitaminosis D in comparison to individuals with ASD and NT controls. Associations between vitamin D deficiency and high BMI, personal autoimmunity, and familial autoimmunity were present in individuals with DS.

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Section: Discussionmentioning

confidence: 99%

Hypovitaminosis D in persons with Down syndrome and autism spectrum disorder

Boyd,

Nguyen,

Khoshnood

et al. 2023

J Neurodevelop Disord

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“…However, the underlying mechanisms of the PEP1 peptide’s involvement in immunoregulatory responses and immune-related signal pathways is still unclear. DCs are the most effective APCs that can be recognized by extracellular immunopeptides and bound by MHC-II molecules; this complex can present on the surface of the APCs and bind to the TCR on T-cells to activate the immune response [ 25 , 26 ]. Therefore, cell phenotype analysis of DC2.4 and the transcriptomic study were used to uncover the mechanism of the PEP1 peptide.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Immunoregulatory Activity of Rice Seed-Derived Peptide PEP1 on Dendritic Cells

et al. 2023

Molecules

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Some food-derived bioactive peptides exhibit prominent immunoregulatory activity. We previously demonstrated that the rice-derived PEP1 peptide, GIAASPFLQSAAFQLR, has strong immunological activity. However, the mechanism of this action is still unclear. In the present study, full-length transcripts of mouse dendritic cells (DC2.4) treated with PEP1 were sequenced using the PacBio sequencing platform, and the transcriptomes were compared via RNA sequencing (RNA-Seq). The characteristic markers of mature DCs, the cluster of differentiation CD86, and the major histocompatibility complex (MHC-II), were significantly upregulated after the PEP1 treatment. The molecular docking suggested that hydrogen bonding and electrostatic interactions played important roles in the binding between PEP1, MHC-II, and the T-cell receptor (TCR). In addition, the PEP1 peptide increased the release of anti-inflammatory factors (interleukin-4 and interleukin-10) and decreased the release of pro-inflammatory factors (interleukin-6 and tumor necrosis factor-α). Furthermore, the RNA-seq results showed the expression of genes involved in several signaling pathways, such as the NF-κB, MAPK, JAK-STAT, and TGF-β pathways, were regulated by the PEP1 treatment, and the changes confirmed the immunomodulatory effect of PEP1 on DC2.4 cells. This findings revealed that the PEP1 peptide, derived from the byproduct of rice processing, is a potential natural immunoregulatory alternative for the treatment of inflammation.

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Transcriptomic analysis reveal the responses of dendritic cells to VDBP

Cited by 2 publications

References 71 publications

Hypovitaminosis D in persons with Down syndrome and autism spectrum disorder

Hypovitaminosis D in persons with Down syndrome and autism spectrum disorder

Transcriptome Analysis Reveals the Immunoregulatory Activity of Rice Seed-Derived Peptide PEP1 on Dendritic Cells

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