Transcriptomic and biochemical analysis of metabolic remodeling in Bacillus subtilis MSC4 under Benzo[a]pyrene stress

Chen, Rui; Liu, Na; Ren, Yuan; Cui, Tangbing

doi:10.1016/j.chemosphere.2024.141637

Chemosphere

2024

DOI: 10.1016/j.chemosphere.2024.141637

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Transcriptomic and biochemical analysis of metabolic remodeling in Bacillus subtilis MSC4 under Benzo[a]pyrene stress

Rui Chen,

Na Liu,

Yuan Ren

et al.

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2024

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Cited by 2 publications

References 64 publications

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MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1

Zhou,

Chen,

et al. 2024

Agriculture

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The purpose of this study is to discover and excavate more key factors and signaling pathways that regulate the formation intensity of biofilms and to fully reveal the possible models affecting biofilm formation. By using gene homologous recombination and bioinformatics technology, a MutL protein-directed deletion mutant strain was successfully constructed. The growth status of the mutant strain was observed, and it was confirmed that, except for the change in cell morphology, there were no significant differences in growth and reproduction between the mutant strain and the wild-type strain. By using the induced biofilm formation technique, the significant decrease in biofilm formation in the MutL mutant strain was successfully verified. The plate confrontation test confirmed that the inhibitory ability of the mutant strain against rice blast fungus was not significantly different from that of the WT strain. The colonization ability of the mutant strain on rice stems was tested, and it was confirmed that the colonization ability of the mutant strain was significantly lower than that of the WT strain. In terms of the prevention and control effect of rice blast disease, the mutant strain showed a significant decrease. By using transcriptomic big data, the gene and pathway expression differences between the mutant strain and the WT strain during biofilm formation were analyzed. The analysis revealed no significant correlation with the previously reported spo0A and tapA-sipW-tasA pathways. The key factor capB of the polyglutamic acid signaling pathway, which affects the formation of the biological model, was found to have a significant decrease in expression. A mechanical hypothesis was proposed: MutL may participate in regulating the formation intensity of Bacillus biofilms by regulating the formation of glutamic acid to polyglutamic acid.

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MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1

Zhou,

Chen,

et al. 2024

Agriculture

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Functional and Proteomic Analyses of a Putative Carbamoyl Phosphate Synthase Large Subunit in Relation to Virulence, Arginine and Pyrimidine Biosynthesis, and Siderophore Production in Erwinia amylovora

Han,

Lee,

Kim

et al. 2024

Horticulturae

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The apple is a significant global fruit cultivated extensively worldwide. Fire blight, caused by Erwinia amylovora (Ea), poses a significant threat to global apple production. To control this disease, characterizing the virulence mechanisms/factors is imperative. Carbamoyl phosphate synthase is an essential enzyme in the biosynthesis of arginine and pyrimidine. However, the functions of this protein in Ea remains poorly understood. This study aimed to investigate the functions of the carbamoyl phosphate synthase large subunit in Ea (CarBEa). In a virulence assay using fruitlets, an Ea strain lacking CarBEa exhibited significantly reduced virulence on fruitlets. In the auxotrophy assay, this mutant failed to grow in minimal media lacking both arginine and pyrimidine, but growth was restored when both compounds were supplemented. The comparative proteomic analysis suggests that CarBEa is involved in diverse biological processes, including amino acid and nucleotide metabolism, and inorganic ion transport. Finally, we demonstrated that CarBEa is related to siderophore secretion/production by the chrome azurol S agar plate assay. This report provides valuable insights into the functions of carbamoyl phosphate synthase large subunit, which serves as a potential target for developing efficient anti-virulence substances to control fire blight.

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Transcriptomic and biochemical analysis of metabolic remodeling in Bacillus subtilis MSC4 under Benzo[a]pyrene stress

Cited by 2 publications

References 64 publications

MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1

MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1

Functional and Proteomic Analyses of a Putative Carbamoyl Phosphate Synthase Large Subunit in Relation to Virulence, Arginine and Pyrimidine Biosynthesis, and Siderophore Production in Erwinia amylovora

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