Transcriptomic and epigenomic insights into pectoral muscle fiber formation at the late embryonic development in pure chicken lines

Gu, Shuang; Huang, Qiang; Sun, Congjiao; Wen, Chaoliang; Yang, Ning

doi:10.1016/j.psj.2024.103882

Poultry Science

2024

DOI: 10.1016/j.psj.2024.103882

|View full text |Cite

Transcriptomic and epigenomic insights into pectoral muscle fiber formation at the late embryonic development in pure chicken lines

Shuang Gu,

Qiang Huang,

Congjiao Sun

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed

Zhang,

Li,

et al. 2024

Poultry Science

View full text Add to dashboard Cite

Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed

Zhang,

Li,

et al. 2024

Poultry Science

View full text Add to dashboard Cite

Comparative Analysis of Myofiber Characteristics, Shear Force, and Amino Acid Contents in Slow- and Fast-Growing Broilers

Gu,

Gao,

et al. 2024

Foods

View full text Add to dashboard Cite

Skeletal muscle fiber characteristics are pivotal in assessing meat quality. However, there is currently a lack of research precisely quantifying the total number of myofibers (TNM) of skeletal muscles. This study used Arbor Acres (AA) broilers and Wenchang (WC) chickens to determine the TNM of several skeletal muscles and the meat quality of the pectoralis major muscle (PM). The results showed that the TNMs of the PM in AA males and females were 935,363.64 ± 92,529.28 and 873,983.72 ± 84,511.28, respectively, significantly higher than those in WC (511,468.97 ± 73,460.81 and 475,371.93 ± 70,187.83) at 7 days of age (p < 0.01). In terms of gastrocnemius medialis in AA males and females, we recorded values of 207,551.43 ± 31,639.97 and 177,203.23 ± 28,764.01, showing a significant difference compared to the values observed in WC (146,313.03 ± 29,633.21 and 124,238.9 ± 20,136.95) (p < 0.01). Similarly, the levels of gastrocnemius lateralis exhibited a significant difference between AA and WC (p < 0.01). Furthermore, the essential, umami, and sweet amino acids were found to be significantly higher in WC compared to AA (p < 0.01). These findings offer valuable data and insights for accurately quantifying the TNM in livestock and for the development of further genetic breeding strategies for meat quality.

show abstract

Exploring Gene Expression and Alternative Splicing in Duck Embryonic Myoblasts via Full-Length Transcriptome Sequencing

Wu,

Liu,

Jiang

et al. 2024

Veterinary Sciences

View full text Add to dashboard Cite

The duck industry is vital for supplying high-quality protein, making research into the development of duck skeletal muscle critical for improving meat and egg production. In this study, we leveraged Oxford Nanopore Technologies (ONT) sequencing to perform full-length transcriptome sequencing of myoblasts harvested from the leg muscles of duck embryos at embryonic day 13 (E13), specifically examining both the proliferative (GM) and differentiation (DM) phases. Our analysis identified a total of 5797 novel transcripts along with 2332 long non-coding RNAs (lncRNAs), revealing substantial changes in gene expression linked to muscle development. We detected 3653 differentially expressed genes and 2246 instances of alternative splicing, with key genes involved in essential pathways, such as ECM–receptor interaction and Notch signaling, prominently featured. Additionally, we constructed a protein–protein interaction network that highlighted critical regulators—MYOM3, MYL2, MYL1, TNNI2, and ACTN2—associated with the processes of proliferation and differentiation in myoblasts. This extensive transcriptomic investigation not only sheds light on the intricate molecular mechanisms driving skeletal muscle development in ducks but also provides significant insights for future breeding strategies aimed at enhancing the efficiency of duck production. The results emphasize the efficacy of ONT sequencing in uncovering complex regulatory networks within avian species, ultimately contributing to progress in animal husbandry.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Transcriptomic and epigenomic insights into pectoral muscle fiber formation at the late embryonic development in pure chicken lines

Cited by 3 publications

References 80 publications

Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed

Identification of key differentially methylated genes regulating muscle development in chickens: insights from Jingyuan breed

Comparative Analysis of Myofiber Characteristics, Shear Force, and Amino Acid Contents in Slow- and Fast-Growing Broilers

Exploring Gene Expression and Alternative Splicing in Duck Embryonic Myoblasts via Full-Length Transcriptome Sequencing

Contact Info

Product

Resources

About