Transcriptomic and metabolic regulatory network characterization of drought responses in tobacco

Abstract

Drought stress usually causes huge economic losses for tobacco industries. Drought stress exhibits multifaceted impacts on tobacco systems through inducing changes at different levels, such as physiological and chemical changes, changes of gene transcription and metabolic changes. Understanding how plants respond and adapt to drought stress helps generate engineered plants with enhanced drought resistance. In this study, we conducted multiple time point-related physiological, biochemical,transcriptomic and metabolic assays using K326 and its derived mutant 28 (M28) with contrasting drought tolerance. Through integrative analyses of transcriptome and metabolome,we observed dramatic changes of gene expression and metabolic profiles between M28 and K326 before and after drought treatment. we found that some of DEGs function as key enzymes responsible for ABA biosynthesis and metabolic pathway, thereby mitigating impairment of drought stress through ABA signaling dependent pathways. Four DEGs were involved in nitrogen metabolism, leading to synthesis of glutamate (Glu) starting from NO−3 /NO−2 that serves as an indicator for stress responses. Importantly, through regulatory network analyses, we detected several drought induced TFs that regulate expression of genes responsible for ABA biosynthesis through network, indicating direct and indirect involvement of TFs in drought responses in tobacco. Thus, our study sheds some mechanistic insights into how plant responding to drought stress through transcriptomic and metabolic changes in tobacco. It also provides some key TF or non-TF gene candidates for engineering manipulation for breeding new tobacco varieties with enhanced drought tolerance.

Integrated analysis of transcriptomics and metabolomics of garden asparagus (Asparagus officinalis L.) under drought stress

“…In contrast, genes and metabolites related to sugar metabolism were less changed in ‘Pacific Early’, indicating that ‘Pacific Early’ failed to produce more osmoregulatory substances to resist drought stress. The TCA cycle is an important component of plant energy metabolism [ 21 , 23 , 47 ]. In this study, in ‘Jilv3’ under drought stress, the TCA cycle was enhanced by the upregulation of related DEGs, including SDH , CS , SDH , and IDH , which could efficiently improve the utilization of malic acid, citric acid, and succinic acid.…”

“…Under drought stress, metabolic processes, such as the citrate cycle (TCA cycle), glucose metabolism, and glycolysis/gluconeogenesis, change to different degrees. These metabolisms correspond closely to drought resistance [ 21 – 23 ].…”

MicroRNAs: Potential prognostic and theranostic biomarkers in chronic lymphocytic leukemia

“…One of the regulators of the BCR signaling pathway is miR‐150. Several studies have shown that this miRNA adjusts the level of the FOXP1 transcription factor in CLL cells [61‐63]. FOXP1 is an essential factor in the BCR, NF‐κB, and Wnt signaling [64, 65] and its overexpression is associated with several B lymphocyte malignancies and adverse outcomes in the corresponding patients [64, 66].…”

Genome-wide identification and expression analysis of the ADH gene family under diverse stresses in tobacco (Nicotiana tabacum L.)

“…The ADH gene family plays vital roles in plant growth and development, as well as in responding to various stresses such as low temperature [ 8 ], drought [ 9 ], salt [ 10 ], mechanical damage [ 11 ], and the exogenous hormone abscisic acid [ 12 ]. In Panax ginseng , the positive response of PgADHs to abiotic stresses, including ABA, SA, and JA treatment, suggests that PgADHs are genuinely involved in these hormone-related stress responses [ 13 ].…”