Transcriptomic and metabolomic analyses reveal antibacterial mechanism of astringent persimmon tannin against Methicillin-resistant Staphylococcus aureus isolated from pork

Liu, Miaomiao; Feng, Mingxing; Yang, Kun; Cao, Yangchun; Zhang, Jie; Xu, Junnan; Hernández, Santiago Hernández; Wei, Xinyuan; Fan, Mingtao

doi:10.1016/j.foodchem.2019.125692

Cited by 95 publications

(67 citation statements)

References 32 publications

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“…A recent metatranscriptomics assessment of the diversity and expression levels of resistance genes in the gut microbiome of birds with aquatic habits revealed a strong effect of the environment while eliciting the expression of AMR traits [157]. Moreover, transcriptomics approach was employed in the comparative evaluation of the expression profile of methicillin-resistant Staphylococcus aureus isolated from pork [158]. The study highlights that administration of subinhibitory doses of antibacterial compounds provoke alteration of the transcriptional profile, with genes related to membrane transport, amino acids, carbohydrate, and energy metabolism being differentially expressed.…”

Section: Latest Frontiers On Veterinary Amr Researchmentioning

confidence: 99%

Antimicrobial Resistance in Veterinary Medicine: An Overview

Palma

Tilocca

Roncada

2020

IJMS

183

100

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Antimicrobial resistance (AMR) represents one of the most important human- and animal health-threatening issues worldwide. Bacterial capability to face antimicrobial compounds is an ancient feature, enabling bacterial survival over time and the dynamic surrounding. Moreover, bacteria make use of their evolutionary machinery to adapt to the selective pressure exerted by antibiotic treatments, resulting in reduced efficacy of the therapeutic intervention against human and animal infections. The mechanisms responsible for both innate and acquired AMR are thoroughly investigated. Commonly, AMR traits are included in mobilizable genetic elements enabling the homogeneous diffusion of the AMR traits pool between the ecosystems of diverse sectors, such as human medicine, veterinary medicine, and the environment. Thus, a coordinated multisectoral approach, such as One-Health, provides a detailed comprehensive picture of the AMR onset and diffusion. Following a general revision of the molecular mechanisms responsible for both innate and acquired AMR, the present manuscript focuses on reviewing the contribution of veterinary medicine to the overall issue of AMR. The main sources of AMR amenable to veterinary medicine are described, driving the attention towards the indissoluble cross-talk existing between the diverse ecosystems and sectors and their cumulative cooperation to this warning phenomenon.

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Section: Latest Frontiers On Veterinary Amr Researchmentioning

confidence: 99%

Antimicrobial Resistance in Veterinary Medicine: An Overview

Palma

Tilocca

Roncada

2020

IJMS

183

100

View full text Add to dashboard Cite

show abstract

“…More importantly, compared with conventional assays, application of omics technologies has greater potential to explore the new action targets of antimicrobials (Santos et al, 2016). The omic approaches have been adopted to investigate the antibacterial mechanism of many natural compounds, including usnic acid (Xu et al, 2017), alpha‐mangostin (Sivaranjani et al, 2019), berberine (Du et al, 2020), cuminal (Zhang et al, 2020), and tannin (Liu et al, 2020). In the present study, a combination of transcriptome and proteome analyses was used to quantify the changes in expression level of genes and proteins in S .…”

Section: Discussionmentioning

confidence: 99%

Discovering the antibacterial mode of action of 3‐p‐trans‐coumaroyl‐2‐hydroxyquinic acid, a natural phenolic compound, against Staphylococcus aureus through an integrated transcriptomic and proteomic approach

Liu

Yue

et al. 2020

Journal of Food Safety

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A plant‐derived phenolic compound, namely 3‐p‐trans‐coumaroyl‐2‐hydroxyquinic acid (CHQA), has recently been reported to exhibit antibacterial and antibiofilm activities against Staphylococcus aureus. In this study, the combined transcriptomic and proteomic analyses was used to elucidate the molecular mechanism of CHQA against S. aureus. The results showed that subinhibitory concentration of CHQA induced wide and significant changes in S. aureus at both transcriptional and translational levels with 935 differentially expressed genes and 438 differentially expressed proteins. Bioinformatic analysis indicated that the changed genes and proteins were mainly involved in cell membrane, ribosome and translation, DNA repair, fatty acid biosynthesis and metabolism, amino acid biosynthesis, peptidoglycan synthesis, and bacterial infection. Moreover, downregulation of various surface proteins associated with cell adhesion was observed, which probably further inhibited the biofilm formation of S. aureus. These results revealed that CHQA exerted antibacterial activity through multifarious mechanisms, which especially targeted bacterial cell membrane and then triggered diverse cellular dysfunctions.

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“…These contained amino acid ABC transporter ATP-binding protein, ABC transporter ATP-binding protein, peptide ABC transporter permease, exconuclease ABC, spermidine/putrescine ABC transporter permease. ABC transporter systems are associated with nutrient uptake and the export of toxins and antibiotics, and they also have a potential pathogenic role during host infection (42). Previous study has proved that peptide ABC transporter permease, excinuclease ABC and spermidine/putrescine ABC transporter permease are pathogenesis-related ABC transporters and important for Salmonella in vivo infection.…”

Section: Discussionmentioning

confidence: 99%