Transcriptomic and Metabolomic Analysis of Seedling-Stage Soybean Responses to PEG-Simulated Drought Stress

Wang, Xiyue; Song, Shuang; Wang, Xin; Liu, Jun; Dong, Shoukun

doi:10.3390/ijms23126869

Cited by 41 publications

(30 citation statements)

References 38 publications

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“…The integrated transcriptome and metabolomic analysis of sesame emphasized the significance of amino acid metabolism, especially the saccharopine pathway, ABA metabolism, and signaling pathway for drought resistance ( You et al., 2019 ). The identification of several candidate genes and metabolites associated with isoflavone synthesis and the tricarboxylic acid cycle further supports the significance of these metabolic pathways in the response of soybean to drought ( Wang et al., 2022 ). Similarly, the role of metabolites (phenolics and phenylpropanoids) has been identified in maize under biotic and abiotic stresses ( Block et al., 2020 ).…”

Section: Multi-omics Techniques Accelerate the Genetic Dissection Of ...mentioning

confidence: 59%

Multi-omics revolution to promote plant breeding efficiency

Mahmood

Li²,

Fan³

et al. 2022

Front. Plant Sci.

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Crop production is the primary goal of agricultural activities, which is always taken into consideration. However, global agricultural systems are coming under increasing pressure from the rising food demand of the rapidly growing world population and changing climate. To address these issues, improving high-yield and climate-resilient related-traits in crop breeding is an effective strategy. In recent years, advances in omics techniques, including genomics, transcriptomics, proteomics, and metabolomics, paved the way for accelerating plant/crop breeding to cope with the changing climate and enhance food production. Optimized omics and phenotypic plasticity platform integration, exploited by evolving machine learning algorithms will aid in the development of biological interpretations for complex crop traits. The precise and progressive assembly of desire alleles using precise genome editing approaches and enhanced breeding strategies would enable future crops to excel in combating the changing climates. Furthermore, plant breeding and genetic engineering ensures an exclusive approach to developing nutrient sufficient and climate-resilient crops, the productivity of which can sustainably and adequately meet the world’s food, nutrition, and energy needs. This review provides an overview of how the integration of omics approaches could be exploited to select crop varieties with desired traits.

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Section: Multi-omics Techniques Accelerate the Genetic Dissection Of ...mentioning

confidence: 59%

Multi-omics revolution to promote plant breeding efficiency

Mahmood

Li²,

Fan³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…It is broadly applied to track transcriptomic variation in plants, in response to abiotic and biotic stresses. Moreover, metabolomic analysis provides valuable information on system-wide changes in plant metabolism, and allows for the identification of compounds with key roles in plant stress tolerance [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L

Xiong

2022

IJMS

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Carbon nanoparticles have potential threats to plant growth and stress tolerance. The polyhydroxy fullerene—fullerol (one of the carbon nanoparticles) could increase biomass accumulation in several plants subjected to drought; however, the underlying molecular and metabolic mechanisms governed by fullerol in improving drought tolerance in Brassica napus remain unclear. In the present study, exogenous fullerol was applied to the leaves of B. napus seedlings under drought conditions. The results of transcriptomic and metabolomic analyses revealed changes in the molecular and metabolic profiles of B. napus. The differentially expressed genes and the differentially accumulated metabolites, induced by drought or fullerol treatment, were mainly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to carbohydrate metabolism (e.g., “carbon metabolism” and “galactose metabolism”), amino acid metabolism (e.g., “biosynthesis of amino acids” and “arginine and proline metabolism”), and secondary metabolite metabolism (e.g., “biosynthesis of secondary metabolites”). For carbohydrate metabolism, the accumulation of oligosaccharides (e.g., sucrose) was decreased, whereas that of monosaccharides (e.g., mannose and myo-inositol) was increased by drought. With regard to amino acid metabolism, under drought stress, the accumulation of amino acids such as phenylalanine and tryptophan decreased, whereas that of glutamate and proline increased. Further, for secondary metabolite metabolism, B. napus subjected to soil drying showed a reduction in phenolics and flavonoids, such as hyperoside and trans-3-coumaric acid. However, the accumulation of carbohydrates was almost unchanged in fullerol-treated B. napus subjected to drought. When exposed to water shortage, the accumulation of amino acids, such as proline, was decreased upon fullerol treatment. However, that of phenolics and flavonoids, such as luteolin and trans-3-coumaric acid, was enhanced. Our findings suggest that fullerol can alleviate the inhibitory effects of drought on phenolics and flavonoids to enhance drought tolerance in B. napus.

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“…There have been many studies focusing on the physiological responses of plants under drought stress, including the production of reactive oxygen species (Zhanassova et al, 2021), changes in enzyme activity (Moloi et al, 2016), accumulation of osmoregulatory substances (Lotfi et al, 2010;Lu et al, 2019). changes in pigment content (Saha et al, 2020) and related molecular processes (Wang et al, 2022), etc. Resistant and sensitive varieties may differ in several of these indexes, however, the ultimate difference is either survival or death at a certain critical moisture or a certain critical stress time.…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of drought tolerance of spring soybean at seedling stage under climate change

Wang

Li²,

Dong

2022

Front. Sustain. Food Syst.

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Drought is one of the major abiotic stress factors limiting soybean growth and yield, and it frequently occur globally. Therefore, exploring resistant varieties from soybean germplasm is important under climate change. To screen drought resistant spring soybean varieties at seedling stage, pot experiment was used to detect the Survival percentage after drought stress of 60 soybean varieties at seedling stage, twice drought rehydration treatments on seedlings, to evaluate the drought tolerance of spring soybean. The results showed that at the seedling stage, seven varieties were considered drought tolerant, 17 varieties were considered drought sensitive, and 36 varieties were considered to be moderately drought tolerant. Based on this experiment, number 44 (heinong37), 48 (heinong44), 49 (heinong45), 52 (heinong48) is considered the best drought resistant, and number 3 (dongnong48), 4 (dongnong52), 27 (suinong25), 60 (heinong65) is the most sensitive. These varieties provide a reference for further study on drought tolerance and stress resistance gene screening of soybean at the molecular level. The selected soybean varieties can be planted in areas with suitable climates and frequent drought to meet the local soybean demand. In other regions, although cannot be directly grown, they can still be used as parents of selected varieties or as materials for gene screening and extraction, to assist crop breeding at the molecular level in response to increasingly severe drought stress problems under the current climate trends.

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Transcriptomic and Metabolomic Analysis of Seedling-Stage Soybean Responses to PEG-Simulated Drought Stress

Cited by 41 publications

References 38 publications

Multi-omics revolution to promote plant breeding efficiency

Multi-omics revolution to promote plant breeding efficiency

Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L

Screening and identification of drought tolerance of spring soybean at seedling stage under climate change

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