Transcriptomic and proteomic analyses of venom glands from scorpions Liocheles australasiae, Mesobuthus martensii, and Scorpio maurus palmatus

So, Wai Lok; Leung, Thomas; Nong, Wenyan; Bendena, William G.; Ngai, Sai Ming; Hui, Jerome H. L.

doi:10.1016/j.peptides.2021.170643

Cited by 8 publications

(7 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…San’ao decoction with scorpion has been shown to relieve asthma ( Wang P. et al, 2021 ). It is well known that scorpion venom is the leading cause of human poisoning and even death by scorpion sting ( So et al, 2021 ). Reports in recent years indicated that scorpion venom might be the main active ingredient in scorpion that exerts anticancer effects.…”

Section: Discussionmentioning

confidence: 99%

Astragalus–Scorpion Drug Pair Inhibits the Development of Prostate Cancer by Regulating GDPD4-2/PI3K/AKT/mTOR Pathway and Autophagy

You

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Objective: Prostate cancer (PCa) is an epithelial malignancy of the prostate that currently lacks effective treatment. Traditional Chinese medicine (TCM) can play an anticancer role through regulating the immune system, anti-tumor angiogenesis, regulating tumor cell apoptosis, autophagy dysfunction, and other mechanisms. This study attempted to explore the active ingredients and potential mechanism of action of the Astragalus–Scorpion (A–S) drug pair in PCa, in order to provide new insights into the treatment of PCa.Methods: Network pharmacology was used to analyze the A–S drug pair and PCa targets. Bioinformatics analysis was used to analyze the LncRNAs with significant differences in PCa. The expression of LC3 protein was detected by immunofluorescence. CCK8 was used to detect cell proliferation. The expressions of GDPD4-2, AC144450.1, LINC01513, AC004009.2, AL096869.1, AP005210.1, and BX119924.1 were detected by RT-qPCR. The expression of the PI3K/AKT/mTOR pathway and autophagy-related proteins were detected by western blot. LC-MS/MS was used to identify the active components of Astragalus and Scorpion.Results: A–S drug pair and PCa have a total of 163 targets, which were mainly related to the prostate cancer and PI3K/AKT pathways. A–S drug pair inhibited the formation of PCa, promoted the expression of LC3Ⅱ and Beclin1 proteins, and inhibited the expression of P62 and PI3K–AKT pathway proteins in PCa mice. Astragaloside IV and polypeptide extract from scorpion venom (PESV) were identified as the main active components of the A–S drug pair. GDPD4-2 was involved in the treatment of PCa by Astragaloside IV-PESV. Silencing GDPD4-2 reversed the therapeutic effects of Astragaloside IV-PESV by regulating the PI3K/AKT/mTOR pathway.Conclusion: Astragaloside IV-PESV is the main active components of A–S drug pair treated PCa by regulating the GDPD4-2/PI3K–AKT/mTOR pathway and autophagy.

show abstract

Section: Discussionmentioning

confidence: 99%

Astragalus–Scorpion Drug Pair Inhibits the Development of Prostate Cancer by Regulating GDPD4-2/PI3K/AKT/mTOR Pathway and Autophagy

You

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Among them, serine proteases are frequently present, but in a low proportion, in the toxic secretions of these and other buthid scorpions (Estrada-Gómez et al, 2017). This situation contrasts with their predominant presence in the venom of non-buthid species (Carmo et al, 2014;Lok So et al, 2021).…”

Section: Venom Compositionmentioning

confidence: 92%

“…The consistent records of PLA 2 in the venom gland transcriptome of several scorpion species (Rodríguez de la Vega et al, 2010; Ma et al, 2012) suggest that these enzymes are also ubiquitous in the different lineages of the Order, although in Tityus they represent less than 1% of the transcripts (de . Thus, most of these phospholipases may have lost their enzymatic activity or the remaining scaffolds could have developed new functions associated with, for instance, the induction of in ammation with reactions that often accompany envenomings induced by scorpions and other arthropods (Lok So et al, 2021).…”

Section: Venom Compositionmentioning

confidence: 99%

“…As in all members of the Buthidae family, cysteine-containing peptides dominate in T. ocelote venom. These molecules are responsible for the neurotoxic effects commonly associated with the sting of buthids, modifying the function of ion channels in excitable and non-excitable tissues (Lok So et al, 2021). A diversity of peptides affects different ion channels, but those that modulate sodium (NaTxs) and potassium (KTx) channels, prevail in Buthidae (Lok So et al, 2021).…”

Section: Venom Compositionmentioning

confidence: 99%

See 1 more Smart Citation

Venomics of the Scorpion Tityus ocelote (Scorpiones, Buthidae): Understanding Venom Evolution in the Subgenus Archaeotityus

Díaz

Chang-Castillo

Lomonte

et al. 2022

Int J Pept Res Ther

View full text Add to dashboard Cite

The subgenus Tityus (Archaeotityus) includes small variegated species considered until recently, a basal group within Tityus, one of the most diverse and medically relevant genera of scorpions in the New World. Archaeotityus species are distributed in the humid forests of Lower Central America and the Choco, Guiana Shield, and Amazon regions. Due to their size and habits, Archaeotityus species are not usually considered dangerous, however, there are some clinical reports that show otherwise. To contribute to the toxinological knowledge of these poorly explored species, we characterized the venom of Tityus ocelote from three localities in Costa Rica. In addition, we assessed the evolutionary relationships of putative sodium channel modulating peptides found in this species with those reported for other members of the genus, through a maximum likelihood phylogenetic analysis based on their amino acid sequences.We observed the presence of homologs of previously identi ed NaTx from the species T. (Archaeotityus) clathratus and some other putative Na + and K + channel modulating peptides related to the T. bahiensis group. In addition, we sequenced some peptides related to toxins present in the venom of the subgenus Atreus, such as those reported T. obscurus, T. pachyurus, and the Costa Rican endemic species T. dedoslargos. Our phylogenetic analysis suggests that the venom of this Archaeotityus species is very complex and that some of the ion channel toxins expressed in it are related to distinct lineages within the genus Tityus, which could represent a plesiomorphic condition conserved in this group of scorpions of the New World.

show abstract

“…Other rare types of venom of the scorpion species present in France were also studied. They were limited to Isometrus maculatus [ 88 ] and Liocheles australasiae [ 89 ] venom. For other scorpion species, the venom has not been studied, but for some of them, the venom of scorpions of the same genus have be studied ( Centruroides [ 90 , 91 , 92 , 93 , 94 , 95 , 96 ], Opisthacanthus [ 97 , 98 ], Didymocentrus [ 99 ], Hormurus [ 100 , 101 ]).…”

Section: Scorpion Venommentioning

confidence: 99%

French Scorpionism (Mainland and Oversea Territories): Narrative Review of Scorpion Species, Scorpion Venom, and Envenoming Management

Vaucel

Larréché

Paradis

et al. 2022

Toxins

View full text Add to dashboard Cite

Sixty-seven scorpion species have been described in France and its territories, where they have been found to be heterogeneously distributed. Indeed, only one species can be found on Réunion Island, while 38 species exist in French Guiana. The number of stings is also heterogenous, with up to 90 stings per 100,000 inhabitants occurring annually. Scorpion species can frequently be determined through simple visual factors, including species of medical importance (i.e., Buthus, Centruroides and Tityus). Scorpion venom is composed of local enzymes and peptides with a cysteine-stabilized α/β motif (NaTxs, Ktxs, Calcines), which allow for venom diffusion and the prey’s incapacitation, respectively. Harmful scorpion species are limited to Centruroides pococki in the French West Indies, which can induce severe envenoming, and the Tityus obscurus and Tityus silvestris in French Guiana, which can cause fatalities in children and can induce severe envenoming, respectively. Envenomation by one of these scorpions requires hospital monitoring as long as systemic symptoms persist. Typical management includes the use of a lidocaine patch, pain killers, and local antiseptic. In the case of heart failure, the use of dobutamine can improve survival, and pregnant women must consult an obstetrician because of the elevated risk of preterm birth or stillbirth. France does not have scorpion antivenom, as scorpion stings are generally not fatal.

show abstract

Transcriptomic and proteomic analyses of venom glands from scorpions Liocheles australasiae, Mesobuthus martensii, and Scorpio maurus palmatus

Cited by 8 publications

References 59 publications

Astragalus–Scorpion Drug Pair Inhibits the Development of Prostate Cancer by Regulating GDPD4-2/PI3K/AKT/mTOR Pathway and Autophagy

Astragalus–Scorpion Drug Pair Inhibits the Development of Prostate Cancer by Regulating GDPD4-2/PI3K/AKT/mTOR Pathway and Autophagy

Venomics of the Scorpion Tityus ocelote (Scorpiones, Buthidae): Understanding Venom Evolution in the Subgenus Archaeotityus

French Scorpionism (Mainland and Oversea Territories): Narrative Review of Scorpion Species, Scorpion Venom, and Envenoming Management

Contact Info

Product

Resources

About