Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector

Nekkanti, Aarthi; Chakraborty, Prosenjit; Ghosh, Amalendu; Iquebal, Mir Asif; Jaiswal, Sarika

doi:10.3389/fmicb.2022.890807

Cited by 5 publications

(7 citation statements)

References 85 publications

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“…In our previous study, the expression of B. tabaci TLR3 and TOB1 was found highly abundant in response to ChiLCV infection ( Nekkanti et al., 2022 ). In the present study, dsRNAs were designed to knock down B. tabaci TLR3 and TOB1 .…”

Section: Methodsmentioning

confidence: 89%

“…Silencing of B. tabaci hsp70 and fasciclin 2 ( fas2) modulates the ChiLCV transmission ( Chakraborty and Ghosh, 2022 ). In our previous study, the differentially expressed genes (DEGs) of B. tabaci in response to ChiLCV have been reported ( Nekkanti et al., 2022 ). DEGs associated with innate immunity such as Toll-like receptor 3 (TLR3) , fas2 , transducer of erbB2.1 ( TOB1 ), and GMP reductase were highly abundant.…”

Section: Introductionmentioning

confidence: 99%

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Effect of silencing Bemisia tabaci TLR3 and TOB1 on fitness and begomovirus transmission

et al. 2023

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Bemisia tabaci (Hemiptera: Aleyrodidae) is one of the most important invasive pests worldwide. It infests several vegetables, legumes, fiber, and ornamental crops. Besides causing direct damage by sucking plant sap, B. tabaci is the principal vector of begomoviruses. Chilli leaf curl virus (ChiLCV, Begomovirus) transmitted by B. tabaci is a major constraint in chilli production. B. tabaci genes associated with metabolism, signaling pathways, cellular processes, and organismal systems are highly enriched in response to ChiLCV infection. The previous transcriptome study suggested the association of B. tabaci Toll-like receptor 3 (TLR3) and transducer of erbB2.1 (TOB1) in ChiLCV infection. In the present study, B. tabaci TLR3 and TOB1 were silenced using double-stranded RNA (dsRNA) and the effect on fitness and begomovirus transmission has been reported. Oral delivery of dsRNA at 3 µg/mL reduced the expression of B. tabaci TLR3 and TOB1 by 6.77 and 3.01-fold, respectively. Silencing of TLR3 and TOB1 induced significant mortality in B. tabaci adults compared to untreated control. The ChiLCV copies in B. tabaci significantly reduced post-exposure to TLR3 and TOB1 dsRNAs. The ability of B. tabaci to transmit ChiLCV also declined post-silencing TLR3 and TOB1. This is the first-ever report of silencing B. tabaci TLR3 and TOB1 to induce mortality and impair virus transmission ability in B. tabaci. B. tabaci TLR3 and TOB1 would be novel genetic targets to manage B. tabaci and restrict the spread of begomovirus.

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Section: Methodsmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Effect of silencing Bemisia tabaci TLR3 and TOB1 on fitness and begomovirus transmission

et al. 2023

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“…To date, several studies on begomovirus-whitefly interactions have been conducted providing valuable information on the transcriptional variations among different cryptic species of virus-infected whiteflies ( Kaur et al, 2017 ; Geng et al, 2018 ; Hasegawa et al, 2018 ; Ding et al, 2019 ; Li et al, 2020 ; Nekkanti et al, 2022 ). However, the specific information on the CLCuMuV-whitefly interactions remains limited.…”

Section: Discussionmentioning

confidence: 99%

“…For example, comparative transcriptomic profiling of MEAM1 whiteflies infected with a begomovirus (Tomato yellow leaf curl virus, TYLCV) and a crinivirus (Tomato chlorosis virus, ToCV) reveals a number of transcription factors, receptors, cathepsins and hemocyanin genes involved in regulation of the insect antiviral immunity along with their possible role in the virus transmission ( Hasegawa et al, 2018 ). Another study describes the transcriptomic changes of Asia II 1 whiteflies after infection with a begomovirus (Chilli leaf curl virus, ChiLCV) revealing several candidate genes associated with virus infection and its circulative movement in the insect vector ( Nekkanti et al, 2022 ). Likewise, the transcriptional changes in the guts of TYLCV-infected MEAM1 whiteflies showed that TYLCV could activate insect immune responses and several other genes associated with intracellular signaling ( Geng et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling reveals a network of differentially expressed genes in Asia II 7 and MEAM1 whitefly cryptic species in response to early infection of Cotton leaf curl Multan virus

Farooq

She

et al. 2022

Front. Microbiol.

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Cotton leaf curl Multan virus (CLCuMuV) is a whitefly-vectored begomovirus that poses ramping threat to several economically important crops worldwide. The differential transmission of CLCuMuV by its vector Bemisia tabaci mainly relies on the type of whitefly cryptic species. However, the molecular responses among different whitefly cryptic species in response to early CLCuMuV infection remain elusive. Here, we compared early-stage transcriptomic profiles of Asia II 7 and MEAM1 cryptic species infected by CLCuMuV. Results of Illumina sequencing revealed that after 6 and 12 h of CLCuMuV acquisition, 153 and 141 genes among viruliferous (VF) Asia II 7, while 445 and 347 genes among VF MEAM 1 whiteflies were differentially expressed compared with aviruliferous (AVF) whiteflies. The most abundant groups of differentially expressed genes (DEGs) among Asia II 7 and MEAM1 were associated with HTH-1 and zf-C2H2 classes of transcription factors (TFs), respectively. Notably, in contrast to Asia II 7, MEAM1 cryptic species displayed higher transcriptional variations with elevated immune-related responses following CLCuMuV infection. Among both cryptic species, we identified several highly responsive candidate DEGs associated with antiviral innate immunity (alpha glucosidase, LSM14-like protein B and phosphoenolpyruvate carboxykinase), lysosome (GPI-anchored protein 58) and autophagy/phagosome pathways (sequestosome-1, cathepsin F-like protease), spliceosome (heat shock protein 70), detoxification (cytochrome P450 4C1), cGMP-PKG signaling pathway (myosin heavy chain), carbohydrate metabolism (alpha-glucosidase), biological transport (mitochondrial phosphate carrier) and protein absorption and digestion (cuticle protein 8). Further validation of RNA-seq results showed that 23 of 28 selected genes exhibited concordant expression both in RT-qPCR and RNA-seq. Our findings provide vital mechanistic insights into begomovirus-whitefly interactions to understand the dynamics of differential begomovirus transmission by different whitefly cryptic species and reveal novel molecular targets for sustainable management of insect-transmitted plant viruses.

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“…Using various omics platforms, including transcriptomics, the virus-associated microeffects of begomoviruses and criniviruses have been examined for B. tabaci (MEAM1 and MED) [44][45][46][47][48][49][50][51][52][53][54]. However, what remains unclear is whether micro-effects are due to the direct effect of the virus on the vector or to the indirect effects resulting from the modulation of the physiological changes in the infected host plant following virus infection.…”

Section: Introductionmentioning

confidence: 99%

Differential Transcriptional Responses in Two Old World Bemisia tabaci Cryptic Species Post Acquisition of Old and New World Begomoviruses

Mugerwa

Gautam

Catto

et al. 2022

Cells

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Begomoviruses are transmitted by several cryptic species of the sweetpotato whitefly, Bemisia tabaci (Gennadius), in a persistent and circulative manner. Upon virus acquisition and circulative translocation within the whitefly, a multitude of molecular interactions occur. This study investigated the differentially expressed transcript profiles associated with the acquisition of the Old World monopartite begomovirus, tomato yellow leaf curl virus (TYLCV), and two New World bipartite begomoviruses, sida golden mosaic virus (SiGMV) and cucurbit leaf crumple virus (CuLCrV), in two invasive B. tabaci cryptic species, Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED). A total of 881 and 559 genes were differentially expressed in viruliferous MEAM1 and MED whiteflies, respectively, compared with their non-viruliferous counterparts, of which 146 genes were common between the two cryptic species. For both cryptic species, the number of differentially expressed genes (DEGs) associated with TYLCV and SiGMV acquisition were higher compared with DEGs associated with CuLCrV acquisition. Pathway analysis indicated that the acquisition of begomoviruses induced differential changes in pathways associated with metabolism and organismal systems. Contrasting expression patterns of major genes associated with virus infection and immune systems were observed. These genes were generally overexpressed and underexpressed in B. tabaci MEAM1 and MED adults, respectively. Further, no specific expression pattern was observed among genes associated with fitness (egg production, spermatogenesis, and aging) in viruliferous whiteflies. The weighted gene correlation network analysis of viruliferous B. tabaci MEAM1 and MED adults identified different hub genes potentially implicated in the vector competence and circulative tropism of viruses. Taken together, the results indicate that both vector cryptic species and the acquired virus species could differentially affect gene expression.

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Transcriptomic Changes of Bemisia tabaci Asia II 1 Induced by Chilli Leaf Curl Virus Trigger Infection and Circulation in Its Vector

Cited by 5 publications

References 85 publications

Effect of silencing Bemisia tabaci TLR3 and TOB1 on fitness and begomovirus transmission

Effect of silencing Bemisia tabaci TLR3 and TOB1 on fitness and begomovirus transmission

Comparative transcriptome profiling reveals a network of differentially expressed genes in Asia II 7 and MEAM1 whitefly cryptic species in response to early infection of Cotton leaf curl Multan virus

Differential Transcriptional Responses in Two Old World Bemisia tabaci Cryptic Species Post Acquisition of Old and New World Begomoviruses

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