Transcriptomic landscape of hepatic lymph nodes, peripheral blood lymphocytes and spleen of swamp buffaloes infected with the tropical liver fluke Fasciola gigantica

Hu, Rui-Si; Zhang, Fu-Kai; Ma, Qiao-Ni; Ehsan, Muhammad; Zhao, Quan; Zhu, Xing‐Quan

doi:10.1371/journal.pntd.0010286

Cited by 4 publications

(2 citation statements)

References 58 publications

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“…Overall, the enriched pathways and processes indicate that an immune response was happening in the sheep treated with commercial vaccines. In livestock, few studies have been made on the spleen transcriptome after an infection and the enriched terms in these works were similar to those detected in the present study ( 39 – 42 ).…”

Section: Discussionsupporting

confidence: 87%

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Guisasola-Serrano,

Bilbao-Arribas,

Varela-Martínez

et al. 2024

Front. Immunol.

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Aluminum hydroxide has long been employed as a vaccine adjuvant for its safety profile, although its precise mechanism of action remains elusive. In this study, we investigated the transcriptomic responses in sheep spleen following repetitive vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. Notably, this work represents the first exploration of the sheep spleen transcriptome in such conditions. Animals were splitted in 3 treatment groups: vaccine group, adjuvant alone group and control group. A total of 18 high-depth RNA-seq libraries were sequenced, resulting in a rich dataset which also allowed isoform-level analysis. The comparisons between vaccine-treated and control groups (V vs C) as well as between vaccine-treated and adjuvant-alone groups (V vs A) revealed significant alterations in gene expression profiles, including protein coding genes and long non-coding RNAs. Among the differentially expressed genes, many were associated with processes such as endoplasmic reticulum (ER) stress, immune response and cell cycle. The analysis of co-expression modules further indicated a correlation between vaccine treatment and genes related to ER stress and unfolded protein response. Surprisingly, adjuvant-alone treatment had little impact on the spleen transcriptome. Additionally, the role of alternative splicing in the immune response was explored. We identified isoform switches in genes associated with immune regulation and inflammation, potentially influencing protein function. In conclusion, this study provides valuable insights into the transcriptomic changes in sheep spleen following vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. These findings shed light on the molecular mechanisms underlying vaccine-induced immune responses and emphasize the significance of antigenic components in aluminum adjuvant mechanism of action. Furthermore, the analysis of alternative splicing revealed an additional layer of complexity in the immune response to vaccination in a livestock species.

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Section: Discussionsupporting

confidence: 87%

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Guisasola-Serrano,

Bilbao-Arribas,

Varela-Martínez

et al. 2024

Front. Immunol.

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“…Furthermore, ref. [ 55 ] reported that the ADH5 gene may be related to selection for growth in Nellore cattle. All these ADH genes were detected in our work, but enriched for different pathways, such as FA metabolism, pyruvate metabolism, and glycolysis.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle

Reis,

Carvalho,

Espigolan

et al. 2023

Metabolites

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The meat market has enormous importance for the world economy, and the quality of the product offered to the consumer is fundamental for the success of the sector. In this study, we analyzed a database which contained information on 2470 animals from a commercial farm in the state of São Paulo, Brazil. Of this total, 2181 animals were genotyped, using 777,962 single-nucleotide polymorphisms (SNPs). After quality control analysis, 468,321 SNPs provided information on the number of genotyped animals. Genome-wide association analyses (GWAS) were performed for the characteristics of the rib eye area (REA), subcutaneous fat thickness (SFT), shear force at 7 days’ ageing (SF7), and intramuscular fat (IMF), with the aid of the single-step genomic best linear unbiased prediction (ssGBLUP) method, with the purpose of identifying possible genomic windows (~1 Mb) responsible for explaining at least 0.5% of the genetic variance of the traits under analysis (≥0.5%). These genomic regions were used in a gene search and enrichment analyses using MeSH terms. The distributed heritability coefficients were 0.14, 0.20, 0.18, and 0.21 for REA, SFT, SF7, and IMF, respectively. The GWAS results indicated significant genomic windows for the traits of interest in a total of 17 chromosomes. Enrichment analyses showed the following significant terms (FDR ≤ 0.05) associated with the characteristics under study: for the REA, heat stress disorders and life cycle stages; for SFT, insulin and nonesterified fatty acids; for SF7, apoptosis and heat shock proteins (HSP27); and for IMF, metalloproteinase 2. In addition, KEGG (Kyoto encyclopedia of genes and genomes) enrichment analysis allowed us to highlight important metabolic pathways related to the studied phenotypes, such as the growth hormone synthesis, insulin-signaling, fatty acid metabolism, and ABC transporter pathways. The results obtained provide a better understanding of the molecular processes involved in the expression of the studied characteristics and may contribute to the design of selection strategies and future studies aimed at improving the productivity of Nellore cattle.

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Immune modulation of goat monocytes by Fasciola gigantica Legumain-1 protein (Fg-LGMN-1)

Ehsan,

Hu,

Wang

et al. 2024

Experimental Parasitology

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Transcriptomic landscape of hepatic lymph nodes, peripheral blood lymphocytes and spleen of swamp buffaloes infected with the tropical liver fluke Fasciola gigantica

Cited by 4 publications

References 58 publications

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Identifying transcriptomic profiles in ovine spleen after repetitive vaccination

Genome-Wide Association (GWAS) Applied to Carcass and Meat Traits of Nellore Cattle

Immune modulation of goat monocytes by Fasciola gigantica Legumain-1 protein (Fg-LGMN-1)

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