Transcriptomic Landscape of Medicinal Dendrobium Reveals Genes Associated With the Biosynthesis of Bioactive Components

Wang, Zhicai; Zhao, Miaomiao; Cui, Hongqiu; Li, Jian; Wang, Meina

doi:10.3389/fpls.2020.00391

Cited by 34 publications

(40 citation statements)

References 138 publications

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“…Dendrobine (20) belongs to the class of terpenoid indole alkaloids (TIAs) ( Wang et al, 2020 ). The biogenetic pathway of TIAs is conservative among alkaloid-producing plants ( Li Q. et al, 2017 ).…”

Section: Chemical Synthesis and Biosynthetic Pathway Of Dendrobinementioning

confidence: 99%

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Mou

Zhao

et al. 2021

Front. Pharmacol.

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Dendrobium is a genus of flowering plants belonging to the Orchidaceae family with more than 1,400 species. Many Dendrobium species have been used as medicinal plants in several Asian countries for thousands of years. Alkaloids were reported as the major biological markers due to their complex chemical compositions and various types. In this review, we summarized the structural types of alkaloids, their pharmacological activities, as well as the mechanisms of biological activities. More than sixty alkaloids were isolated and identified from the Dendrobium genus. Moreover, the pharmacological effects of Dendrobium alkaloids as hepatic lipid and gluconeogenesis regulation, as neuroprotection, and as anti-tumor, anti-inflammatory, anti-diabetes, and anti-virus factors were described. Besides, the total chemical synthesis of dendrobine is provided, while the biosynthetic pathway of dendrobine has been proposed based on the functions of associated genes. For applications of these invaluable herbs, more researches on the extraction of biological markers from compounds are needed. Further confirmation of the proposed biosynthetic pathways is anticipated as well.

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Section: Chemical Synthesis and Biosynthetic Pathway Of Dendrobinementioning

confidence: 99%

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Mou

Zhao

et al. 2021

Front. Pharmacol.

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“…In plant breeding and horticulture, transcriptome sequencing is highly employed for predicting novel genes, gene function, and genome evolution [ 11 ]. Here, we performed whole-transcriptome sequencing to study the mechanism of purple color development in D. nestor petals, which is the first report, as compared to other Dendrobium species [ 13 , 69 , 70 , 71 , 72 ]. H and B samples might have relatively similar anthocyanins accumulation and synthesis, hence the high similarity as evident in Figure 1 a,b and Figure 4 b.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of the Anthocyanin Biosynthetic Pathway Reveals the Molecular Mechanism Associated with Purple Color Formation in Dendrobium Nestor

Cui

Jieling

Huang

et al. 2021

Life

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Dendrobium nestor is a famous orchid species in the Orchidaceae family. There is a diversity of flower colorations in the Dendrobium species, but knowledge of the genes involved and molecular mechanism underlying the flower color formation in D. nestor is less studied. Therefore, we performed transcriptome profiling using Illumina sequencing to facilitate thorough studies of the purple color formation in petal samples collected at three developmental stages, namely—flower bud stage (F), half bloom stage (H), and full bloom stage (B) in D. nestor. In addition, we identified key genes and their biosynthetic pathways as well as the transcription factors (TFs) associated with purple flower color formation. We found that the phenylpropanoid–flavonoid–anthocyanin biosynthesis genes such as phenylalanine ammonia lyase, chalcone synthase, anthocyanidin synthase, and UDP-flavonoid glucosyl transferase, were largely up-regulated in the H and B samples as compared to the F samples. This upregulation might partly account for the accumulation of anthocyanins, which confer the purple coloration in these samples. We further identified several differentially expressed genes related to phytohormones such as auxin, ethylene, cytokinins, salicylic acid, brassinosteroid, and abscisic acid, as well as TFs such as MYB and bHLH, which might play important roles in color formation in D. nestor flower. Sturdy upregulation of anthocyanin biosynthetic structural genes might be a potential regulatory mechanism in purple color formation in D. nestor flowers. Several TFs were predicted to regulate the anthocyanin genes through a K-mean clustering analysis. Our study provides valuable resource for future studies to expand our understanding of flower color development mechanisms in D. nestor.

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“…Chemical isolation of compounds has identified diverse active compounds including polysaccharides, flavonoids, alkaloids, and multiple amino acids. In particular, the polysaccharides, alkaloids, and flavonoids extracted from D. officinale are thought to be the main bioactive ingredients for medicine ( Meng et al, 2016 ; Tang et al, 2017 ; Wang et al, 2020 ; Wang, 2021 ). Pharmacological studies have confirmed the positive effects of polysaccharides, alkaloids and flavonoids extracted from D. officinale on immune regulation and liver protection ( Wang et al, 2020 ), on anti-cancer and neuroprotection ( Zhang et al, 2003 ; Wang et al, 2020 ), and on anti-oxidant activity ( Yuan et al, 2020a ).…”

Section: Introductionmentioning

confidence: 99%

Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale

et al. 2022

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Dendrobium officinale Kimura et Migo is an important epiphytic plant, belonging to the Orchidaceae family. There are various bioactive components in D. officinale plants, mainly including polysaccharides, alkaloids, and phenolic compounds. These compounds have been demonstrated to possess multiple functions, such as anti-oxidation, immune regulation, and anti-cancer. Due to serious shortages of wild resources, deterioration of cultivated germplasm and the unstable quality of D. officinale, the study has been focused on the biosynthetic pathway and regulation mechanisms of bioactive compounds. In recent years, with rapid developments in detection technologies and analysis tools, omics research including genomics, transcriptomics, proteomics and metabolomics have all been widely applied in various medicinal plants, including D. officinale. Many important advances have been achieved in D. officinale research, such as chromosome-level reference genome assembly and the identification of key genes involved in the biosynthesis of active components. In this review, we summarize the latest research advances in D. officinale based on multiple omics studies. At the same time, we discuss limitations of the current research. Finally, we put forward prospective topics in need of further study on D. officinale.

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Transcriptomic Landscape of Medicinal Dendrobium Reveals Genes Associated With the Biosynthesis of Bioactive Components

Cited by 34 publications

References 138 publications

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Transcriptomic Analysis of the Anthocyanin Biosynthetic Pathway Reveals the Molecular Mechanism Associated with Purple Color Formation in Dendrobium Nestor

Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale

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