Transcriptomic response of rat hippocampus and spleen cells to single and chronic administration of the peptide selank

Kolomin, Timur; Шадрина, М. И.; Agniullin, Ya. V.; Shram, S. I.; Сломинский, П. А.; Limborska, Svetlana A.; Myasoedov, N. F.

doi:10.1134/s1607672910010023

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…It was shown that Cx3cr1 gene expression after Selank introduction is considerably stronger in the rat spleen than it is in the brain. The strongest increase in mRNA levels was observed after a single injection of the peptide (by 16-fold) [184].…”

Section: Effect Of Selank On the Transcriptomementioning

confidence: 96%

“…Concomitantly, administration of a single intranasal dose of Selank (200 μg/kg) changed the mRNA levels of 36 genes, and a curative administration of Selank (200 μg/kg once a day for 5 days) changed the mRNA levels of 20 genes. The expression level of six genes (Actn1, Cx3cr1, Fgf7, Kng1, Ptprn2, and Slc6a20) changed after both single and curative introduction of Selank [184].…”

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

“…The third cluster included 15 genes encoding proteins involved in the cellular transport system: Arfgap1, Atp5a1, Cacna1g, Clcnka, Gria4, Grid2, Kcnj4, Scamp5, Scn3b, Slc1a2, Slc5a7, Slc6a20, Slc8a3, Trpc1, and Tomm20. Of these, 10 are involved exclusively in ion transport and ion homeostasis for cell support: Atp5a1, Cacna1g, Clcnka, Gria4, Grid2, Kcnj4, Scn3b, Slc5a7, Slc8a3, and Trpc1 [184,187].…”

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

“…Significantly higher levels of the Cx3cr1 mRNA were detected in the frontal cortex and cerebellum after a single Selank injection, whereas multidirectional changes in the mRNA level of this gene were observed in these regions of the brain after curative administration of the peptide. In the rat cerebellum, a significant decrease was observed in the mRNA levels of the Ptprn2 gene, which is widely expressed in the brain and is involved in the growth and differentiation of nerves [184].…”

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

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A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

Kolomin¹,

Шадрина²,

Slominsky³

et al. 2013

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Natural regulatory peptides are biologically active compounds that are produced by various cells and provide a link among the main regulatory systems of the body. The field of research into the biologic activity of endogenous regulatory peptides is extremely vast. These peptides affect the cardiovascular, immune, reproductive, endocrine, digestive, and other systems, alter energy metabolism, and are especially effective in the regulation of the central nervous system. Despite of the wide range of preventive and therapeutic effects of natural regulatory peptides and proteins, their application in clinical practice is difficult. This is primarily because of their extreme instability, as they are rapidly degraded by proteases of the gastrointestinal tract, blood, cerebrospinal fluid, and other biologic media. Compounds with higher stability (i.e., a considerably longer half-life compared with that of natural molecules) and the ability to provide a directional effect on the various body systems were obtained from modifications of endogenous regulatory peptides. Synthetic analogs of regulatory peptides, as a rule, contain only natural amino acids in their composition, and their biodegradation does not lead to the formation of toxic products; thus, they have fewer side effects. This review focuses on the consideration of two synthetic regulatory peptides, Semax and Selank, which were the bases for the creation of new drugs that are used effectively in the treatment of various diseases of the nervous system. The synthetic analog of an adrenocorticotropic hormone 4-10 fragment (ACTH 4-10) Semax is a powerful neuroprotective agent that is particularly effective as a therapy for stroke. Selank was synthesized on the basis of the natural immunomodulator tuftsin. Selank is a powerful anxiolytic that is used as a therapy for generalized anxiety disorder and neurasthenia without sedative and muscle-relaxant effects. This review presents the results of research aimed at studying the influence of these peptides on the transcriptome of brain cells. The problems of drugs developed based on the clinical activities of Semax and Selank are discussed separately.

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Section: Effect Of Selank On the Transcriptomementioning

confidence: 96%

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

Section: Effect Of Selank On the Transcriptomementioning

confidence: 99%

See 2 more Smart Citations

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

Kolomin¹,

Шадрина²,

Slominsky³

et al. 2013

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“…Previously, it was shown that Selank causes a marked change in the expression of genes involved in inflammatory processes in the hippocampus and spleen of rodents (Kolomin et al, 2010 , 2011 , 2014 ). Our results have confirmed at the molecular level that the clinical effects observed after the introduction of Selank are related to its antiviral activity (Ershov et al, 2009 ; Andreeva et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

et al. 2017

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Clinical studies have shown that Selank had an anxiolytic effect comparable to that of classical benzodiazepine drugs, which can enhance the inhibitory effect of GABA by allosteric modulation of GABAA receptors. These data suggest that the molecular mechanism of the effect of Selank may also be related to its ability to affect the performance of the GABAergic system. To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method. As test substances, in addition to Selank, we selected the major GABAA receptor ligand, GABA, the atypical antipsychotic, olanzapine, and combinations of these compounds (Selank and GABA; Selank and olanzapine). We found no changes in the mRNA levels of the genes studied under the effect of Selank. The combined effect of GABA and Selank led to nearly complete suppression of changes in expression of genes in which mRNA levels changed under the effect of GABA. When Selank was used in conjunction with olanzapine, the expression alterations of more genes were observed compared with olanzapine alone. The data obtained indicate that Selank has no direct effect on the mRNA levels of the GABAergic system genes in neuroblastoma IMR-32 cells. At the same time, our results partially confirm the hypothesis that the peptide may affect the interaction of GABA with GABAA receptors. Our data also suggest that Selank may enhance the effect of olanzapine on the expression of the genes studied.

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Pharmacotranscriptomics of peptide drugs with neuroprotective properties

Dergunova

Filippenkov

Limborska

et al. 2020

Medicinal Research Reviews

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Here we present a review of studies on the effects of peptides with neuroprotective properties on gene transcription in nerve cells. The few published works in this area clearly demonstrate massive changes in cell transcriptomes induced by peptides under normal conditions and under conditions of experimental brain ischemia. These changes significantly affect signaling and metabolic pathways, affecting various body systems and confirming the multiple target actions of peptides. The importance of noncoding RNAs in the regulation of these processes is shown, and we discuss the prospects of research for determining the main mechanisms of peptide regulation, which is necessary for the further development of drugs with targeted neuroprotective effects.

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Transcriptomic response of rat hippocampus and spleen cells to single and chronic administration of the peptide selank

Cited by 7 publications

References 12 publications

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

A New Generation of Drugs: Synthetic Peptides Based on Natural Regulatory Peptides

GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells

Pharmacotranscriptomics of peptide drugs with neuroprotective properties

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