Transcriptomic responses of the freshwater snail (Parafossarulus striatulus) following dietary exposure to cyanobacteria

Qiao, Fei; Lei, Kun; Li, Zicheng; Wei, Zhanliang; Liu, Qing; Yang, Lo; He, JianWu; An, Lihui; Qi, Hongli; Cui, Song

doi:10.1016/j.scitotenv.2017.12.112

Cited by 20 publications

(12 citation statements)

References 37 publications

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“…Cyanobacteria are widespread throughout aquatic areas and are the main source of energy for snails (Qiao et al, 2018). Cyanobacteria were the second most dominant bacterial taxa in our study: 147 OTUs were assigned to Cyanobacteria, which represented 12.3% of the total OTUs and 17.9% of the total abundance.…”

Section: Discussionmentioning

confidence: 99%

Compositional and predicted functional analysis of the gut microbiota of Radix auricularia (Linnaeus) via high-throughput Illumina sequencing

Chen

Chang

et al. 2018

PeerJ

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Due to its wide distribution across the world, the snail Radix auricularia plays a central role in the transferal of energy and biomass by consuming plant biomass in freshwater systems. The gut microbiota are involved in the nutrition, digestion, immunity, and development of snails, particularly for cellulolytic bacteria, which greatly contribute to the digestion of plant fiber. For the first time, this study characterized the gut bacterial communities of R. auricularia, as well as predicted functions, using the Illumina Miseq platform to sequence 16S rRNA amplicons. Both juvenile snails (JS) and adult snails (AS) were sampled. The obtained 251,072 sequences were rarefied to 214,584 sequences and clustered into 1,196 operational taxonomic units (OTUs) with 97% sequence identity. The predominant phyla were Proteobacteria (JS: 36.0%, AS: 31.6%) and Cyanobacteria (JS: 16.3%, AS: 19.5%), followed by Chloroflexi (JS: 9.7%, AS: 13.1%), Firmicutes (JS: 14.4%, AS: 6.7%), Actinobacteria (JS: 8.2%, AS: 12.6%), and Tenericutes (JS: 7.3%, AS: 6.2%). The phylum Cyanobacteria may have originated from the plant diet instead of the gut microbiome. A total of 52 bacterial families and 55 genera were found with >1% abundance in at least one sample. A large number of species could not be successfully identified, which could indicate the detection of novel ribotypes or result from insufficient availability of snail microbiome data. The core microbiome consisted of 469 OTUs, representing 88.4% of all sequences. Furthermore, the predicted function of bacterial community of R. auricularia performed by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States suggests that functions related to metabolism and environmental information processing were enriched. The abundance of carbohydrate suggests a strong capability of the gut microbiome to digest lignin. Our results indicate an abundance of bacteria in both JS and AS, and thus the bacteria in R. auricularia gut form a promising source for novel enzymes, such as cellulolytic enzymes, that may be useful for biofuel production. Furthermore, searching for xenobiotic biodegradation bacteria may be a further important application of these snails.

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Section: Discussionmentioning

confidence: 99%

Compositional and predicted functional analysis of the gut microbiota of Radix auricularia (Linnaeus) via high-throughput Illumina sequencing

Chen

Chang

et al. 2018

PeerJ

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“…Cyanotoxins, represented by microcystins (MCs), may accumulate in the various tissues of snails through intestinal (consumption) or gill/skin (absorption) epithelia (Massey et al, 2018), resulting in a series of adverse reactions in gastropods (Bownik, 2016). Numerous studies have investigated the response of freshwater snails to cyanobacterial blooms and their toxins from multiple layers and perspectives, including biochemistry and histopathology (Zhu et al, 2011), transcriptomics (Qiao et al, 2018a), metabolomics (Yang M. J. et al, 2022), development (Lei et al, 2017), fecundity (Qiao et al, 2018b), feeding ecology (Qiu et al, 2017), and community dynamics (Gérard and Lance, 2019) and structure (Gérard et al, 2009). However, almost all of these studies have focused on the snails themselves and have failed to consider the dynamics and functions of their symbiotic microbes when exposed to cyanobacterial blooms.…”

Section: Introductionmentioning

confidence: 99%

Responses of Gut Microbial Community Composition and Function of the Freshwater Gastropod Bellamya aeruginosa to Cyanobacterial Bloom

et al. 2022

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Freshwater gastropods are widely distributed and play an important role in aquatic ecosystems. Symbiotic microorganisms represented by gut microbes can affect the physiological and biochemical activities of their hosts. However, few studies have investigated the response of the gut microbial community of snails to environmental stress. In this study, the dynamics of the gut microbiota of the gastropod Bellamya aeruginosa were tracked to explore their responses in terms of their composition and function to cyanobacterial bloom. Differences in gut microbial community structures during periods of non-cyanobacterial bloom and cyanobacterial bloom were determined. Results showed that the alpha diversity of the gut microbiota exposed to cyanobacterial bloom was lower than that of the gut microbiota exposed to non-cyanobacterial bloom. The main genera differentiating the two periods were Faecalibacterium, Subdoligranulum, Ralstonia, and Pelomonas. Microcystins (MCs) and water temperature (WT) were the primary factors influencing the gut microbial community of B. aeruginosa; between them, the influence of MCs was greater than that of WT. Fourteen pathways (level 2) were notably different between the two periods. The pathways of carbohydrate metabolism, immune system, environmental adaptation, and xenobiotics biodegradation and metabolism in these differential pathways exhibited a strong linear regression relationship with MCs and WT. Changes in the functions of the gut microbiota may help B. aeruginosa meet its immunity and energy needs during cyanobacterial bloom stress. These results provide key information for understanding the response pattern of freshwater snail intestinal flora to cyanobacterial blooms and reveal the underlying environmental adaptation mechanism of gastropods from the perspective of intestinal flora.

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“…Therefore, our finding of differential hsa_circ_0001649 expression in EMs represents an initial step toward identifying the factors underlying decreased hsa_circ_0001649 expression. Studies have shown that, in clear cell renal cell carcinoma, breast cancer and granulosa cells, the expression levels of circRNAs are associated with E 2 or Ers (Huang et al 2018, Klinge 2018, Qiao et al 2018. Because E 2 plays key roles in Ems (Huhtinen et al 2012), we suspected and further confirmed experimentally that E 2 can inhibit circRNA expression in ESCs through ERs.…”

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confidence: 52%

Estrogen-decreased hsa_circ_0001649 promotes stromal cell invasion in endometriosis

Liu

et al. 2020

Reproduction

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Endometriosis (EMs) is an estrogen (E2)-dependent inflammatory disorder. Although EMs is considered a benign disease, it presents with malignant characteristics, such as migration and invasion. An increasing number of studies have shown that aberrantly expressed circular RNAs (circRNAs) play an essential role in disease development and progression. However, the mechanisms by which circRNAs exert their pathological effects in EMs remain unclear. Hsa_circ_0001649, a novel cancer-associated circRNA, has been previously reported to be downregulated in several cancer types and related to cell migration and invasion. In the present study, real-time polymerase chain reaction (qRT-PCR) was carried out to measure hsa_circ_0001649 levels in human tissues, human primary endometrial stromal cells (ESCs) and a human endometrial stromal cell line (ThESCs). Matrix metalloproteinase 9 (MMP9) levels in ESCs and ThESCs were assessed by qRT-PCR and western blotting, and the migration and invasion capacities of ThESCs were evaluated by Transwell assay. As a result, hsa_circ_0001649 expression was significantly decreased in ectopic and eutopic endometrial samples compared with that in normal endometrial samples. E2 decreased hsa_circ_0001649 expression but increased MMP9 expression in ESCs and ThESCs. Furthermore, ThESCs were more invasive under E2 stimulation. However, these effects disappeared when ICI or hsa_circ_0001649 transfection was used. Collectively, our findings reveal that decreased hsa_circ_0001649 expression plays a role in E2-increased MMP9 expression through E2 receptors (ERs), which have critical functions in EMs.

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Transcriptomic responses of the freshwater snail (Parafossarulus striatulus) following dietary exposure to cyanobacteria

Cited by 20 publications

References 37 publications

Compositional and predicted functional analysis of the gut microbiota of Radix auricularia (Linnaeus) via high-throughput Illumina sequencing

Compositional and predicted functional analysis of the gut microbiota of Radix auricularia (Linnaeus) via high-throughput Illumina sequencing

Responses of Gut Microbial Community Composition and Function of the Freshwater Gastropod Bellamya aeruginosa to Cyanobacterial Bloom

Estrogen-decreased hsa_circ_0001649 promotes stromal cell invasion in endometriosis

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