Transcriptomics and metabolomics revealed the pulmonary protective mechanism of Xixin-Ganjiang Herb Pair for warming the lungs to dissolve phlegm in COPD rats

Huang, Ping; Duan, Bailu; Li, Deshun; Duan, Yanfen; Zhou, Zhenxiang; Han, Lintao; Li, Jingjing; Wu, Jiajia; Yan, Yonghong; Zhang, Fengyun; Guo, Zhen; Wang, Qiong; Huang, Fang

doi:10.1016/j.jchromb.2023.123665

Cited by 1 publication

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References 53 publications

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“…In our previous study (Huang et al, 2023), 30 active ingredients of XGHP were detected and screened using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) (Figure 2). Then, validated targets for ingredients were obtained using…”

Section: Ultra-performance Liquid Chromatographytandem Mass Spectrome...mentioning

confidence: 99%

Protective effect of Xixin–Ganjiang herb pair for warming the lungs to dissolve phlegm in chronic obstructive pulmonary disease rats based on integrated network pharmacology and metabolomics

Huang,

Xiang,

Wang

et al. 2024

Biomedical Chromatography

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Xixin–Ganjiang herb pair (XGHP) is a classic combination for warming the lungs to dissolve phlegm and is often used to treat a variety of chronic lung diseases; it can treat the syndrome of cold phlegm obstruction of lungs. First, ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) was used to examine the composition of XGHP, and network pharmacology was used to predict its potential core targets and signaling pathways in the current study. Second, a rat model of chronic obstructive pulmonary disease (COPD) was established for assessing the anti‐COPD activity of XGHP, and metabolomics was used to explore the biomarkers and metabolic pathways. Finally, the sample was validated using molecular docking and Western blotting. The integration of metabolomics and network pharmacology results identified 11 targets, 3 biomarkers, 3 pathways, and 2 metabolic pathways. Western blotting showed that XGHP effectively regulated the expression of core proteins via multiple signaling pathways (downregulation of toll‐like receptor 4 [TLR4] and upregulation of serine/threonine‐protein kinase 1 [p‐AKT1] and nitric oxide synthase 3 [NOS3]). Molecular docking results showed that the 10 potentially active components of XGHP have good affinity with tumor necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6), matrix metalloproteinase 9 (MMP‐9), TLR4, p‐AKT1, and NOS3. Our findings suggest that XGHP may regulate glucolipid metabolism, improve energy supply, and inhibit inflammatory responses (TNF‐α, IL‐6, and MMP‐9) via the PI3K‐Akt signaling pathway and HIF‐1 signaling pathway in the management of COPD.

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Section: Ultra-performance Liquid Chromatographytandem Mass Spectrome...mentioning

confidence: 99%

Protective effect of Xixin–Ganjiang herb pair for warming the lungs to dissolve phlegm in chronic obstructive pulmonary disease rats based on integrated network pharmacology and metabolomics

Huang,

Xiang,

Wang

et al. 2024

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

show abstract

Transcriptomics and metabolomics revealed the pulmonary protective mechanism of Xixin-Ganjiang Herb Pair for warming the lungs to dissolve phlegm in COPD rats

Cited by 1 publication

References 53 publications

Protective effect of Xixin–Ganjiang herb pair for warming the lungs to dissolve phlegm in chronic obstructive pulmonary disease rats based on integrated network pharmacology and metabolomics

Protective effect of Xixin–Ganjiang herb pair for warming the lungs to dissolve phlegm in chronic obstructive pulmonary disease rats based on integrated network pharmacology and metabolomics

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