2024
DOI: 10.1101/2024.06.12.598714
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Transcriptomics and proteomics of projection neurons in a circuit linking hippocampus with dorsolateral prefrontal cortex in human brain

Rahul A. Bharadwaj,
Christopher Borcuk,
Gianluca C. Kikidis
et al.

Abstract: RNA-sequencing studies of brain tissue homogenates have shed light on the molecular processes underlying schizophrenia (SCZ) but lack biological granularity at the cell type level. Laser capture microdissection (LCM) can isolate selective cell populations with intact cell bodies to allow complementary gene expression analyses of mRNA and protein. We used LCM to collect excitatory neuron-enriched samples from CA1 and subiculum (SUB) of the hippocampus and layer III of the dorsolateral prefrontal cortex (DLPFC),… Show more

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