Transcripts Expressed during Germination Sensu Stricto Are Associated with Vigor in Soybean Seeds

Ducatti, Karina Renostro; Batista, Thiago Barbosa; Hirai, Welinton Yoshio; Luccas, Daiani Ajala; Moreno, Letícia de Aguila; Guimarães, Cristiane Carvalho; Bassel, George W.; Silva, Edvaldo Aparecido Amaral da

doi:10.3390/plants11101310

Cited by 9 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is confirmed by studies on OGG1 in arabidopsis, where overexpression leads to reduced oxidative damage in dry and hydrated seeds [3]. Furthermore, in soybeans (Glycine max L.), it has been shown that an increase in OGG1 expression results in faster acquisition of germination and thus increased seed vigor [41]. The genes we studied could be used as molecular markers to predict seed quality in a very short time.…”

Section: Discussionsupporting

confidence: 67%

Profiling of Barley, Wheat, and Rye FPG and OGG1 Genes during Grain Germination

Kowalik

Groszyk

2023

IJMS

View full text Add to dashboard Cite

This research is about the profiling of barley (Hordeum vulgare L.), wheat (Triticum aestivum L.), and rye (Secale cereale L.) FPG and OGG1 genes during grain germination. During seed germination, reactive oxygen species accumulate, which leads to DNA damage. In the base excision repair (BER) system, the enzymes formamidopyrimidine DNA glycosylase (FPG) and 8-oxoguanine DNA glycosylase (OGG1), among others, are responsible for repairing such damage. We decided to check how the expression of genes encoding these two enzymes changes in germinating grains. Spring varieties of barley, wheat, and rye from the previous growing season were used in the study. Expression level changes were checked using Real-Time PCR. After analyzing the obtained results, the maximum expression levels of FPG and OGG1 genes during germination were determined for barley, wheat, and rye. The results of the study show differences in expression levels specific to each species. The highest expression was observed at different time points for each of them. There were no differences in the highest expression for FPG and OGG1 within one species. In conclusion, the research provides information on how the level of FPG and OGG1 gene expression changes during the germination process in cereals. This is the first study looking at the expression levels of these two genes in cereals.

show abstract

Section: Discussionsupporting

confidence: 67%

Profiling of Barley, Wheat, and Rye FPG and OGG1 Genes during Grain Germination

Kowalik

Groszyk

2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Therefore, from a seed physiology perspective, the difference in the above traits between seed lots was most likely due to different vigor levels caused by seed aging and deterioration [18]. From the start of seed imbibition to germination or emergence, metabolic repair, including DNA repair, is required and is a necessary step for the subsequent events leading to germination and emergence [55]. Low-vigor seeds germinate more slowly and with more difficulty due to a greater need for damage repair, especially under adverse environmental conditions, which ultimately causes different germination and emergence patterns between seed lots [56].…”

Section: Discussionmentioning

confidence: 99%

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Tao

Chen

Bai

et al. 2023

Plants

View full text Add to dashboard Cite

Seed vigor is an important aspect of seed quality. High-vigor seeds show rapid and uniform germination and emerge well, especially under adverse environmental conditions. Here, we determined hydrotime model parameters by incubating seeds at different water potentials (0.0, −0.2, −0.4, −0.6, and −0.8 MPa) in the laboratory, for 12 seed lots of Chinese milk vetch (Astragalus sinicus) (CMV), a globally important legume used as forage, green manure, and a rotation crop. Pot experiments were conducted to investigate the seedling emergence performance of 12 CMV seed lots under control, water stress, salinity stress, deep sowing, and cold stress conditions. Meanwhile, the field emergence performance was evaluated on two sowing dates in June and October 2022. Correlation and regression analyses were implemented to explore the relationships between hydrotime model parameters and seedling emergence performance under various environmental conditions. The seed germination percentage did not differ significantly between seed lots when seeds were incubated at 0.0 MPa, whereas it did differ significantly between seed lots at water potentials of −0.2, −0.4, and −0.6 MPa. The emergence percentage, seedling dry weight, and simplified vigor index also differed significantly between the 12 seed lots under various environmental conditions. Ψb(50) showed a significant correlation with germination and emergence performance under various environmental conditions; however, little correlation was observed between θH or σφb and germination and emergence. These results indicate that Ψb(50) can be used to estimate seed vigor and predict seedling emergence performance under diverse environmental conditions for CMV and similar forage legumes. This study will enable seed researchers, plant breeders, and government program directors to target higher seed vigor more effectively for forage legumes.

show abstract

“…We previously selected target genes related to longevity and germination using the literature to perform the analysis. Based on the results of the literature analysis, we selected: HSFA3, HSP21, HSP17.6b [ 31 ], EXPA10 [ 32 ], and ABI3 [ 33 ]. The sequence of primers used are listed in Table 5 .…”

Section: Methodsmentioning

confidence: 99%

The Effect of Magneto-Priming on the Physiological Quality of Soybean Seeds

Faria

Santos

Batista

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

Microwaves have been applied to the drying of seeds of several species due to their maintenance of the quality of the seeds and reduction of time and costs. However, few is known about the effect of microwaves on the increase of the physiological quality of soybean seeds and especially their effects on longevity. Therefore, the use of microwaves as magneto-priming in soybean seeds was the object of study in this work. For this purpose, two soybean cultivars were selected and submitted to the ultra-high frequency (UHF) microwave exposure of 2.45 GHz, in the wavelength of 11 cm, and power of 0.2 W/g, for 15 min. The results showed that this condition of exposure to the microwave brought benefits in both cultivars after treatment. Incremental improvements were observed in the germinability indexes, the seedling length, the water absorption by the seeds, the fresh mass, dry mass, and longevity. The genes related to seed germination and longevity showed superior expression (HSFA3, HSP21, HSP17.6b, EXP, ABI3) with magneto-priming treatment. The data found ensure the use of the technique as a viable option for pre-treatment as magneto-priming in soybean seeds in order to improve seed quality.

show abstract

Transcripts Expressed during Germination Sensu Stricto Are Associated with Vigor in Soybean Seeds

Cited by 9 publications

References 31 publications

Profiling of Barley, Wheat, and Rye FPG and OGG1 Genes during Grain Germination

Profiling of Barley, Wheat, and Rye FPG and OGG1 Genes during Grain Germination

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

The Effect of Magneto-Priming on the Physiological Quality of Soybean Seeds

Contact Info

Product

Resources

About