Transcripts for possible capsaicinoid biosynthetic genes are differentially accumulated in pungent and non-pungent Capsicum spp

Curry, Jeanne; Aluru, Maneesha; Mendoza, Marcus; Nevarez, Jacob; Melendrez, Martin; O’Connell, Mary A.

doi:10.1016/s0168-9452(99)00118-1

Cited by 130 publications

(141 citation statements)

References 35 publications

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“…However Curry et al 1999;Aluru et al 2003). If the factors causing high capsaicinoid content in Habanero had have the same effect on capsinoid content, QTL responsible for capsaicinoid might also control accumulation of capsinoid (Blum et al 2003;Ben-chaim et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Substitution of a Dysfunctional pAMT Allele Results in Low-Pungency but High Levels of Capsinoid in Capsicum chinense ‘Habanero’

Jang

Han

et al. 2015

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Capsinoids are the class of secondary metabolites identified in non-pungent peppers exhibiting the same bioactive properties as capsaicinoid. Previously, it has been demonstrated that capsinoid production is controlled by the capsaicin synthase (CS) gene and the putative-aminotransferase (pAMT) gene. In this study, we report that C. chinense 'SNU11-001' containing high levels of capsinoid has an early stop codon in pAMT resulted from 403 bp and 8 bp insertions deletion in the third and sixth exons. In order to know whether CS expression is correlated with the level of capsinoid, CS and pAMT expressions were determined using SNU11-001 and four Capsicum accessions with different pungency level. RT-PCR analysis showed higher transcription levels of CS in pungent accessions but no clear differences in pAMT expression. To investigate the effect of the substitution of the pAMT allele of C. chinense 'Habanero' with the dysfunctional pAMT allele of SNU11-001, an F2 population was constructed by a cross between aforementioned parental lines. Molecular markers were developed to distinguish CS and pAMT genotypes of SNU11-001 and Habanero and F2 plants were genotyped. All F2 plants having the pAMT genotype of SNU11-001 contained high levels of capsinoid while very low levels of capsaicinoid. There was no significant difference in levels of capsinoid among the F2 plants regardless of CS genotypes. This may be due to strong CS expression of both parental lines. In conclusion, our results show that it is possible to develop a new Habanero cultivar with high capsinoid content by introducing a dysfunctional pAMT allele.

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Section: Discussionmentioning

confidence: 99%

Substitution of a Dysfunctional pAMT Allele Results in Low-Pungency but High Levels of Capsinoid in Capsicum chinense ‘Habanero’

Jang

Han

et al. 2015

Plant Breed. Biotech.

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“…Capsaicinoid was first detected at 20th days after anthesis then gradually increased and reached maximal level around 40 d after anthesis, on the 50 d, the capsaicinoid content decreased significantly [6].Capsaicin synthase (CS) is the last enzym in the biosynthesis of capsaicin and have important role in condensation of vanillylamine from phenylpropanoid with 8-methyl-6-nonenoic acid from valine pathway [7]. The CS expression starts at 10 dpa, reaches a maximum at 20 dpa, then suddenly decreases and almost stops at 40 dpa [8].…”

Section: Introductionmentioning

confidence: 99%

Pun1 Gene Isolation from Capsicum frutescens L. cv Cakra Hijau

Khasna¹,

Zairina²,

Juliandari³

et al. 2017

KLS

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Capsicum frutescens L. is one of chili peppers with high pungency. Capsicum frutescens has several cultivars, one of those is C. frutescens cv. Cakra Hijau. This cultivars is known resistance to pests and diseases as well. Pungency is due to the accumulation of capsaicinoids. Pun1 is an important gene responsible for pungency. The full-leght genomic sequence of Pun1 is 1897 bp, containing two exons of 738 bp and 590 bpand one intron of 348 bp in between. This study was aimed to isolate Pun1 gene that free from intron. mRNA was isolated with TriReagent furthermore RT-PCR method used Qiagent One-Step RT-PCR and two pairs of primer : F1/R1 (F15'-ATG-

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“…The O-methylation patterns of polyhydroxylated small molecules are crucial in plants to determine final product distribution via multiple branched biosynthetic pathways using the same or similar intermediates and substrates [20]. Two OMTs expressed in Capsicum placenta tissue, CaOMT1 (U83789.1) from C. annuum [21] and Comt (AF081214.1) from C. chinense [7] have been cloned. Although the participation of Comt in phenylpropanoid-mediated capsaicinoid biosynthesis has long been proposed, it is still unclear whether Comt could be a target for controlling fruit pungency.…”

Section: Introductionmentioning

confidence: 99%

“…The general capsaicinoid biosynthetic pathway has been studied for almost half a century, and transcripts expressed in placenta tissue of pungent cultivars have been isolated [7]- [9].…”

Section: Introductionmentioning

confidence: 99%

“…This result indicates that a loss-of-function mutation in Comt or Kas may induce loss of pungency in Capsicum. Ferulic acid is a precursor of capsaicinoid biosynthesis and is produced from caffeic acid by the action of COMT [5] [7] [17] [18]. COMT is one of the S-adenosyl-L-methionine (SAM)-dependent O-methyltransferases (OMTs) that methylate small molecules involved in the biosynthesis of lignin, flavonoids, alkaloids, and many other plant secondary products [19].…”

Section: Introductionmentioning

confidence: 99%

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A <i>Comt</i>1 Loss of Function Mutation Is Insufficient for Loss of Pungency in <i>Capsicum</i>

Koeda¹,

Sato²,

Tanaka³

et al. 2015

AJPS

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The participation of O-methyltransferase (COMT) in phenylpropanoid-mediated capsaicinoid biosynthesis has long been proposed. Ferulic acid, a phenylpropanoid intermediate, is a precursor of capsaicinoid biosynthesis and is produced from caffeic acid by the action of COMT. As previously reported that silencing Comt expression caused a drastic decrease in capsaicinoid accumulation, it was presumed that a Comt loss-of-function mutation would cause loss of pungency in Capsicum. This hypothesis was tested by cloning Comt1 and Comt2 from the placenta tissue of the pungent cultivar Habanero. The phylogenetic analysis and comparison of critical amino-acid residues for enzyme function showed that the two COMTs had high similarity with the COMTs of other plant species. Moreover, as the two Comts were both expressed in placenta tissue and expressed prior to the accumulation of capsaicinoids, the two genes could be candidates for capsaicinoid biosynthesis. Second, Comt1 loss-of-function mutants were screened from the germplasm. A truncated Comt1 transcript was expressed in non-pungent pepper No.3341 caused by deletion of the genomic region. The predicted No.3341 COMT1 lacked His-265, which was absolutely necessary for enzymatic activity. Contrary to our expectations, the Comt1 mutation was not related to non-pungency of No.3341, as the deletion of Comt1 did not co-segregate with non-pungency in the F2 population obtained from crossing No.3341 with Habanero. This result was confirmed by screening several pungent accessions harboring the same Comt1 deletion mutation. Although the participation of COMT in phenylpropanoid-mediated capsaicinoid biosynthesis has long been proposed, our present study shows that Comt1 can not be a target for controlling fruit pungency.

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Transcripts for possible capsaicinoid biosynthetic genes are differentially accumulated in pungent and non-pungent Capsicum spp

Cited by 130 publications

References 35 publications

Substitution of a Dysfunctional pAMT Allele Results in Low-Pungency but High Levels of Capsinoid in Capsicum chinense ‘Habanero’

Substitution of a Dysfunctional pAMT Allele Results in Low-Pungency but High Levels of Capsinoid in Capsicum chinense ‘Habanero’

Pun1 Gene Isolation from Capsicum frutescens L. cv Cakra Hijau

A <i>Comt</i>1 Loss of Function Mutation Is Insufficient for Loss of Pungency in <i>Capsicum</i>

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Transcripts for possible capsaicinoid biosynthetic genes are differentially accumulated in pungent and non-pungent Capsicum spp

Cited by 130 publications

References 35 publications

Substitution of a Dysfunctional pAMT Allele Results in Low-Pungency but High Levels of Capsinoid in Capsicum chinense ‘Habanero’

Substitution of a Dysfunctional pAMT Allele Results in Low-Pungency but High Levels of Capsinoid in Capsicum chinense ‘Habanero’

Pun1 Gene Isolation from Capsicum frutescens L. cv Cakra Hijau

A &lt;i&gt;Comt&lt;/i&gt;1 Loss of Function Mutation Is Insufficient for Loss of Pungency in &lt;i&gt;Capsicum&lt;/i&gt;

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A <i>Comt</i>1 Loss of Function Mutation Is Insufficient for Loss of Pungency in <i>Capsicum</i>