Transduced PEP-1-PON1 proteins regulate microglial activation and dopaminergic neuronal death in a Parkinson's disease model

Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults

“…In a previous study, we demonstrated that PTD-antioxidant protein successfully transduced into astrocytes and significantly protected against oxidative stress-induced cell death in vitro and in vivo via its antioxidant role [19][20][21]. Moreover, we confirmed that several types of PTD fusion proteins have protective roles against cellular death in vivo and in vitro [22][23][24][25][26][27][28]. In this study, we demonstrate that Tat-PRAS40 protein significantly protects against oxidative stress-induced cellular death, suggesting that Tat-PRAS40 protein may be used as a therapeutic treatment for ischemia.…”

Tat-HSP22 inhibits oxidative stress-induced hippocampal neuronal cell death by regulation of the mitochondrial pathway

“…To detect the intracellular distribution of transduced Tat-HSP22 into HT-22, fluorescence microscopy was performed as described previously [24]. After exposure of HT-22 to Tat-HSP22 (5 μM) for 2 h, the cells were washed twice with PBS.…”

“…To examine the function of Tat-HSP22 against oxidative stress-induced HT-22 cell toxicities, HT-22 were treated with Tat-HSP22 (5 μM) after exposure or non-exposure to hydrogen peroxide for 2 h. Then, the hydrogen peroxide-induced cellular toxicities including cellular ROS (DCF-DA staining), DNA fragmentation (TUNEL staining), and mitochondria dysfunction levels (JC-1 staining) were measured by fluorescence microscopy analysis [22–24, 52]. …”

Efeito da Doença de Parkinson na eficiência dos ciclos mastigatórios

“…Sua etiologia é desconhecida, mas sabe-se que pode ter associação com fatores genéticos, ambientais, estresse oxidativo, químicos e hereditários (FREITAS et al, 2003;SOUZA, et al, 2011;KIM, et al, 2015).…”

“…Nesta doença ocorre à diminuição da produção de dopamina, que é um neurotransmissor que auxilia nos movimentos e coordenação das mudanças de posição corporal (KIM et al, 2015). Os sinais e sintomas característicos da Doença de Parkinson como rigidez muscular, tremor em repouso, bradicinesia, alterações posturais, na marcha (festinada) e fonação estão relacionados com a despigmentação da melatonina nos neurônios dopaminérgicos que ligam o complexo estriado e a substância negra ao córtex cerebral, ocasionando a morte neuronal (QIU, et al, 2015;FERRAZZOLI, et al, 2016).…”