Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)

Cota, Leide Gonçalves; Moreira, Patrícia de Abreu; Menezes, Elytânia Veiga; Gomes, Ana-Margarida; Ericsson, A.R.O.; Oliveira, Dario Alves de; Melo, Afrânio Farias de

doi:10.4238/2012.october.17.7

Cited by 14 publications

(10 citation statements)

References 26 publications

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“…Six microsatellite loci previously developed for A. occidentale (Croxford et al, 2006) and transferred to A. humile (Cota et al, 2012) were used in population genetic analyses ( Table 2). The 15-µL reactions, containing 1X buffer (10 mM Tris-HCl, pH 8.4, 50 mM KCl), 0.7 μM of each primer, 250 μM of each dNTP, 1 U Taq DNA polymerase, 0.25 mg BSA, 1.0 to 1.6 mM MgCl 2 , 1.25 to 3% formamide, and 9 ng DNA were PCR-amplified using a Veriti thermocycler (Applied Biosystems).…”

Section: Sampling Dna Extraction and Microsatellite Analysismentioning

confidence: 99%

Research Article Structure and genetic diversity of Anacardium humile (Anacardiaceae): a tropical shrub.

Cota¹,

Moreira²,

Brandão³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Anacardium humile Saint Hilaire is a tropical shrub native to the Cerrado biome. It is a fruiting species with biological, medicinal, and socioeconomic significance. Thus, knowing how the genetic variability of natural populations is organized allows for the establishment of strategies for conservation and the sustainable use of the species and its biome. Six microsatellite loci previously developed from Anacardium occidentale were used to investigate the spatial genetic structure and genetic diversity of eight natural A. humile populations based on analyses of 242 adult plants. The results obtained indicate that these populations show a high level of genetic diversity (expected heterozygosity = 0.710). The endogamy coefficient was positive and significant for most populations, with a mean of 0.142 (P = 0.001). The genetic differentiation between populations was low (θ = 0.075 and G ST = 0.066) but significant (P = 0.0001). The genotypes of five of the eight populations were non-randomly distributed with clusters of related plants for which the coancestry values were positive and significant. These populations exhibited high and significant endogamy indices. The results obtained for A. humile populations show that genetic conservation programs should be implemented to maintain this species.

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Section: Sampling Dna Extraction and Microsatellite Analysismentioning

confidence: 99%

Research Article Structure and genetic diversity of Anacardium humile (Anacardiaceae): a tropical shrub.

Cota¹,

Moreira²,

Brandão³

et al. 2017

Genet. Mol. Res.

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“…No entanto, diante da carên-cia de informações genéticas sobre esta espécie, alguns estudos vêm sendo desenvolvidos, como os de , que estudaram polimorfismo genético entre populações naturais de A. humile; de Carvalho et al (2012), que analisaram a variabilidade genética da espé-cie com técnica molecular de marcadores RAPD, indicando grande variabilidade genética entre acessos e os estudos de Cota et al (2012) e Soares et al (2013), em que marcadores microssatélites foram transferidos da espécie Anarcadium occidentale para o A. humile, obtendo loci altamente polimórficos que servirão como ferramentas robustas nas análises genéticas de populações desta espécie.…”

Section: Introductionunclassified

Divergência genética por análise multivariada de caracteres fenotípicos de Anacardium humile (St. Hilaire)1

Santos

Júnior

2015

Rev. Ceres

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Divergência genética por análise multivariada de caracteres fenotípicos de Anacardium humile (St. Hilaire) 1 A espécie Anacardium humile (St. Hilaire) é uma fruteira nativa do Cerrado, utilizada tradicionalmente, tanto no consumo in natura como na forma de sucos, doces e geleias, mas ainda pouco explorada economicamente. Em uma área de cerrado campo sujo, com transição para cerrado campo rupestre, localizada no município de Patrocí-nio, Minas Gerais, Brasil, 38 genótipos de uma população dessa espécie foram estudados; com o objetivo de avaliar a diversidade fenotípica, estimada por análise multivariada, bem como características morfológicas promissoras para programas de melhoramento e conservação. Assim, o estudo mostrou variabilidade genética satisfatória dentro da população estudada, destacando-se os genótipos 6, 10, 24, 36 e 38 por elevada produção de frutos e maiores pedúnculos e por se apresentarem distantes geneticamente, sendo indicados para futuros trabalhos de melhoramento genético, para serem multiplicados e utilizados em plantios comerciais e de conservação da espécie.Palavras-chave: Anacardiaceae, caracterização morfológica, variabilidade fenotípica e conservação. Anacardium humile (St. Hilaire) is a fruit native of the Cerrado, traditionally used for both fresh consumption and the preparation of juices, sweets and jellies, but it is insufficiently explored commercially. Thirty eight genotypes of a population of this species were studied in an area of cerrado campo sujo with transition to cerrado campo rupestre in the municipality of Patrocínio, State of Minas Gerais-Brazil. The objective of this study was to evaluate the phenotypic diversity estimated by multivariate analysis and the morphological characteristics that are promising for programs of improvement and conservation. This study showed satisfactory genetic variability within the population studied: the genotypes 6, 10, 24, 36 and 38 showed high production of fruits, weighed cashews apples and genetically distant. These genotypes can be indicated in future breeding programs to be multiplied and used in commercial plantations and conservation of the specie. Rosinalva da Cunha dos

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“…Transferability of microsatellite primers for species within the same genus has been reported previously, such as in Euterpe, E. edulis to E. oleracea, the 7 loci tested presenting 100% success (Oliveira et al, 2010) and Anacardium, A. occidentale, to A. humile, with transferability of all 11 loci analyzed (Cota et al, 2012). Furthermore, rates of polymorphism higher than those observed in this study were obtained by Ince et al (2010a), who detected polymorphisms between the species C. annuum L. and C. frutescens L., with 34 microsatellite primer pairs among the 45 pairs tested.…”

Section: Discussionmentioning

confidence: 86%

“…Several studies have shown high transferability of SSR markers for species within the same genus, including Cucumis (Ritschel et al, 2004), Arachis (Bravo et al, 2006), and even for Capsicum (Yi et al, 2006;Nagy et al, 2007;Ince et al, 2010a). However, the success of this approach depends on the species considered, as the probability of obtaining transferability is inversely proportional to the evolutionary distance among them (Cota et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Transferability of microsatellite markers of Capsicum annuum L. to C. frutescens L. and C. chinense Jacq.

Carvalho¹,

Ragassi²,

Oliveira³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. In order to support further genetic, diversity, and phylogeny studies of Capsicum species, the transferability of a Capsicum annuum L. simple sequence repeat (SSR) microsatellite set was analyzed for C. frutescens L. ("malagueta" and "tabasco" peppers) and C. chinense Jacq. (smell peppers, among other types). A total of 185 SSR primers were evaluated in 12 accessions from 115 C. frutescens L. and 480 C. chinense Jacq, representing different types within each species. Transferability to C. frutescens L. and C. chinense Jacq. occurred for 116 primers (62.7%). Nineteen (16.37%) were polymorphic in C. frutescens L. and 36 (31.03%) in C. chinense Jacq., 17 of which were coincident and could be used to analyze samples obtained for the 2 species.Among these primers, CA49 showed a different amplitude range of alleles between the 2 species (130-132 base pairs for C. frutescens L. and S.I.C. Carvalho et al. 7938©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7937-7946 (2015) 120-128 base pairs for C. chinense Jacq.), and could differentiate the species. A total of 55 alleles were identified among the 19 polymorphic SSR loci among accessions of C. frutescens L., with the number of alleles per locus ranging from 2 to 5, a mean of 2.89, and the polymorphic information content ranging from 0.30 to 0.65. The number of alleles identified in C. chinense Jacq. was 119, ranging from 2 to 5 alleles per locus, an average of 3.30, and polymorphic information content from 0.19 to 0.68. The C. annuum L. SSR primers were most often transferable and polymorphic for C. frutescens L. and C. chinense Jacq., and we present a set of SSR for each species.

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Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)

Cited by 14 publications

References 26 publications

Research Article Structure and genetic diversity of Anacardium humile (Anacardiaceae): a tropical shrub.

Research Article Structure and genetic diversity of Anacardium humile (Anacardiaceae): a tropical shrub.

Divergência genética por análise multivariada de caracteres fenotípicos de Anacardium humile (St. Hilaire)1

Transferability of microsatellite markers of Capsicum annuum L. to C. frutescens L. and C. chinense Jacq.

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