Transferrin Is Not Involved in the Entry of 67Ga into Hepatocytes from Regenerating Liver of Partially Hepatectomized Rats.

Abe, Shinya; Hasegawa, Susumu; Nirasawa, Moriyasu; Sato, Masato; Ohkubo, Yasuhito

doi:10.1248/bpb.24.1343

Cited by 13 publications

(15 citation statements)

References 19 publications

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“…2). This result was consistent with that of CCl 4 -treated rats, 9) and indicated that hepatocyte destruction occurred within 1 d after CCl 4 treatment.The uptake of 67 Ga by the liver tissue increased 1 and 2 d after the treatment (Fig. 3).…”

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“…Hepatocytes were isolated from mice as described previously 18) : briefly, hepatocytes were isolated by a method in which the liver was perfused with collagenase (Nitta Gelatin, Osaka, Japan). The hepatocytes were counted with a well-type NaI-scintillation counter and the results expressed as cpm/1ϫ10 6 cells.…”

Section: Preparation Of Hepatocytesmentioning

confidence: 99%

“…We recently reported that serum ALT level of carbon tetrachloride (CCl 4 ) treated rats reached a maximum within 1 d, whereas maximum uptake of 67 Ga by the liver tissue and hepatocytes was reached in 2 d. 9) Most hepatotoxic effects of CCl 4 are attributed to its metabolism by cytochrome P450, especially by P450 2E1, to yield toxic trichloromethyl radical (CCl 3 *). 10) This radical can react with oxygen to form the trichloromethylperoxy radical (CCl 3 OO*), a highly reactive species.…”

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Uptake of Gallium-67 by the Hepatocytes during Liver Regeneration in Carbon Tetrachloride-Treated Mice

Abe

Suyama

Shishido

et al. 2004

Biological & Pharmaceutical Bulletin

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Gallium-67 ( 67 Ga) has been used as a tumor or inflammation-imaging agent in nuclear medicine. We recently reported that the peak of serum alanine aminotransferase (ALT) level was 1 d after carbon tetrachloride (CCl 4 ) treatment in rats. However, the peak of Ga by the hepatocytes is carried out during liver regeneration rather than during hepatic disorder by CCl 4 treatment. 67 Ga); carbon tetrachloride (CCl 4 ); liver tissue; hepatocyte; lipid peroxidation; bromodeoxyuridine (BrdU) (w/v) trichloroacetic acid, 0.375% (w/v) thiobarbituric acid dissolved in 0.25 M of hydrochloric acid], the mixture was heated for 15 min in a boiling water bath. After cooling, the precipitate was removed by centrifugation at 2000ϫg for 10 min. Following decanting of the supernatant, the absorbance was determined at 535 nm against a blank that contained all the reagents minus sample. The MDA concentration from the samples was calculated using an extinction coefficient of 1.56ϫ10 Key words. The results were expressed as the concentration of MDA/mg protein.Flow Cytometric Analysis of Cell Cycle Cell cycle was analyzed using a BrdU Flow Kit (Becton Dickinson, San Diego, CA, U.S.A.). BrdU was dissolved in phosphate buffered saline (PBS) and was given intravenously (10 mg/kg) 2 h before killing. Incorporation of BrdU into hepatocytes was detected by FACScan (Becton Dickinson). RESULTS Changes in the Lipid Peroxidation of Hepatocytes from CCl 4 -Treated MiceTo ascertain the degree of disorder in the hepatocytes, we determined the lipid peroxidation by thiobarbituric acid assay (Fig. 1). The peroxidation increased after CCl 4 treatment and showed the highest value (7.2 times higher than that of normal mice) 1 d after the treatment.Changes in Serum ALT Level after CCl 4 Treatment Serum ALT level also increased after CCl 4 treatment and reached a maximum in 1 d (Fig. 2). Two days after the treatment the value rapidly decreased. (Fig. 3), while the uptake of 67 Ga by the hepatocytes showed the highest value (8.5 times higher than that of normal mice) 2 d after treatment (Fig. 4). Changes in the Incorporation of Bromodeoxyuridine into Hepatocytes after CCl 4 TreatmentTo confirm DNA synthesis of hepatocytes during liver regeneration after CCl 4 -induced hepatic disorder, we determined their incorporation of BrdU (Fig. 5). Incorporation reached a maximum 2 d after CCl 4 treatment. DISCUSSIONWe recently reported that serum ALT level of CCl 4 -treated rat reached a maximum 1 d after the treatment, whereas the uptake of 67 Ga by the liver tissue and hepatocytes was highest 2 d after treatment. 9)In the present study, we confirmed the precise index of disordered hepatocytes by the degree of lipid peroxidation (Fig. 1). The lipid peroxidation of hepatocytes from CCl 4 -treated mice was greatly increased within 1 d of the treatment. Furthermore, serum ALT (a conventional indicator of hepatic disorder) was also increased within 1 d (Fig. 2). This result was consistent with that of CCl 4 -treated rats, 9) and indicated that hepatocyte destruc...

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Section: Preparation Of Hepatocytesmentioning

confidence: 99%

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Uptake of Gallium-67 by the Hepatocytes during Liver Regeneration in Carbon Tetrachloride-Treated Mice

Abe

Suyama

Shishido

et al. 2004

Biological & Pharmaceutical Bulletin

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“…We have recently suggested that Tf-unbound 67 Ga was taken up into hepatocytes from CCl 4 -treated rat liver. 10) In the present study, we attempted to clarify detailed mechanism of Tf-unbound 67 Ga uptake by hepatocytes treated with CCl 4 using in vitro experimental system.…”

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The Uptake Mechanism of Gallium-67 into Hepatocytes Treated with Carbon Tetrachloride

Abe

Suyama

Chiba

et al. 2007

Biological & Pharmaceutical Bulletin

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“…Moreover we have reported that Tf is involved in 67 Ga uptake by the liver tissue but not in the entry of 67 Ga into the hepatocytes in partially hepatectomized rats. 13) In partial hepatectomy (PH), a part of the blood in circulation is lost together with removed liver tissues, consequently the blood cells and Tf in circulation decrease. Those decreases may cause enhances of the hematopoietic function in bone marrow and the number of Tf receptor (TfR) on various tissues.…”

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The Effect of Blood Decrease on 67Ga Uptake by the Liver in Partially Hepatectomized Rats

Ohtake

Maruko

Satoh

et al. 2007

Biological & Pharmaceutical Bulletin

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Transferrin Is Not Involved in the Entry of 67Ga into Hepatocytes from Regenerating Liver of Partially Hepatectomized Rats.

Cited by 13 publications

References 19 publications

Uptake of Gallium-67 by the Hepatocytes during Liver Regeneration in Carbon Tetrachloride-Treated Mice

Uptake of Gallium-67 by the Hepatocytes during Liver Regeneration in Carbon Tetrachloride-Treated Mice

The Uptake Mechanism of Gallium-67 into Hepatocytes Treated with Carbon Tetrachloride

The Effect of Blood Decrease on 67Ga Uptake by the Liver in Partially Hepatectomized Rats

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