Transformation by viral and cellular oncogenes of a mouse BALB/3T3 cell mutant resistant to transformation by chemical carcinogens.

Ono, Masahiro; Yakushinji, M; Segawa, Kaoru; Kuwano, Michihiko

doi:10.1128/mcb.8.10.4190

Cited by 12 publications

(4 citation statements)

References 48 publications

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“…into cells The cDNA clone of polyoma virus MTAg was linked with mouse mammary tumor virus (MMTV) LTR (Lee et al, 1981). Then, the plasmid, pMMMTAg, was ligated with the DNA fragment (1,400 bp) containing the gene for conferring G418 resistance (Ono et al, 1988). The resulting plasmid, pMMMTAg-neo, was transfected by the method of Graham and Van Der Eb (1973) to stromal cells.…”

Section: Transfection Of Polyoma Middle T Gene (Mtag)mentioning

confidence: 99%

Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo

Umezawa

Maruyama

Segawa

et al. 1992

Journal Cellular Physiology

122

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Several murine marrow stromal cells were established from murine bone marrow cultures. Stromal cell lines transfected with a tumor-inducing polyoma virus middle T antigen (MTAg) were inoculated into nude mice subcutaneously. KUSA-MTAg cells, one of these cell lines, led to the rapid local development of bone marrow consisting of trilineage hematopoietic cells and bone; other cell lines produced spindle cell sarcoma or hemangiosarcoma. These results suggested that a single stromal cell line, KUSA-MTAg cells, may induce hematopoietic stem cells or early progenitors of three lineages of hematopoietic cells in vivo. Interestingly, untransfected KUSA cells expressed three new mesenchymal phenotypes, osteocytes, adipocytes, and myotubes, after treatment with 5-azacytidine.

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Section: Transfection Of Polyoma Middle T Gene (Mtag)mentioning

confidence: 99%

Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo

Umezawa

Maruyama

Segawa

et al. 1992

Journal Cellular Physiology

122

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“…Src family kinases are known to be activated in response to growth factors (20,21), UV irradiation (22), and transition to the M phase in the cell cycle (23)(24)(25). However, it is not fully understood when pp60 c-src and related proto-oncogene products are activated in normal cells.…”

mentioning

confidence: 99%

Activation of pp60 Depending on Cell Density in PC12h Cells

Kobayashi¹,

Okumura²,

Nakamoto³

et al. 1997

Journal of Biological Chemistry

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The Src family tyrosine kinases and their substrates are involved in cell-cell and cell-matrix interactions. We found that in PC12h cells, an increase of cell density enhanced the tyrosine phosphorylation levels of several intracellular proteins including p130 cas . Because it is a possible substrate for Src family kinases, we measured pp60 c-src activity and found that it was higher in high density cultures than in low density cultures. This phenomenon was also observed in PC12 (the parental cell line of the PC12h subclone), Balb/c 3T3, Swiss 3T3, and Hela cells. One of the possible mechanisms regulating the kinase activity of pp60 c-src is the phosphorylation and dephosphorylation of its negative regulatory site located at its C terminus. However, the tyrosine phosphorylation level of the regulatory site did not change depending on cell density. Subcellular fractionation showed that in high density culture, pp60 c-src was translocated from detergent-soluble to detergent-insoluble fractions. These results suggest that cell-cell interaction might induce the activation of pp60 c-src without changing its tyrosine phosphorylation levels.

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“…The malignant transformation system of in vitro Balb/c 3T3 cells is often used in cell transformation experiment [ 14 , 15 ], and it is usually adopted as a classic method in determining carcinogens or cancer promoter. Firstly, 1.2% and 0.6% agarose (Sigma) were prepared with sterile H 2 O and stored in a 4°C refrigerator.…”

Section: Methodsmentioning

confidence: 99%

Effects of Long-Term 50Hz Power-Line Frequency Electromagnetic Field on Cell Behavior in Balb/c 3T3 Cells

Zhou

et al. 2015

PLoS ONE

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Power-line frequency electromagnetic field (PF-EMF) was reported as a human carcinogen by some epidemiological research, but the conclusion is lack of robust experiment evidence. To identify the effects of long-term PF-EMF exposure on cell behavior, Balb/c 3T3 cells in exponential growth phase were exposed or sham-exposed to 50 Hertz (Hz) PF-EMF at 2.3 mT for 2 hours (h) one day, 5 days every week. After 11 weeks exposure, cells were collected instantly. Cell morphology was observed under invert microscope and Giemsa staining, cell viability was detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, cell cycle and apoptosis was examined by flow cytometry, the protein level of Proliferating Cell Nuclear Antigen (PCNA) and CyclinD1 was detected by western blot, cell transformation was examined by soft agar clone assay and plate clone forming test, and cell migration ability was observed by scratch adhesion test. It was found that after PF-EMF exposure, cell morphology, apoptosis, cell migration ability and cell transformation didn’t change. However, compared with sham group, cell viability obviously decreased and cell cycle distribution also changed after 11 weeks PF-EMF exposure. Meanwhile, the protein level of PCNA and CyclinD1 significantly decreased after PF-EMF exposure. These data suggested that although long-term 50Hz PF-EMF exposure under this experimental condition had no effects on apoptosis, cell migration ability and cell transformation, it could affect cell proliferation and cell cycle by down-regulation the expression of PCNA and CyclinD1 protein.

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Transformation by viral and cellular oncogenes of a mouse BALB/3T3 cell mutant resistant to transformation by chemical carcinogens.

Abstract: The mouse cell line MO-5 is resistant to transformation by various chemical carcinogens and also by UV irradiation (C. Yasutake, Y. Kuratomi, M. Ono, S. Masumi, and M. Kuwano, Cancer Res. 47:4894-4899, 1987). Northern (RNA) blot analysis showed active expression of ras and myc genes in

Cited by 12 publications

References 48 publications

Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo

Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo

Activation of pp60 Depending on Cell Density in PC12h Cells

Effects of Long-Term 50Hz Power-Line Frequency Electromagnetic Field on Cell Behavior in Balb/c 3T3 Cells

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