Transforming Chicken Feather Waste into Feather Protein Hydrolysate Using a Newly Isolated Multifaceted Keratinolytic Bacterium Chryseobacterium sediminis RCM-SSR-7

Kshetri, Pintubala; Roy, Suvra; Sharma, Susheel Kumar; Singh, Taranjeet; Ansari, Mukhtar; Prakash, Narendra; Ngachan, S. V.

doi:10.1007/s12649-017-0037-4

Cited by 61 publications

(25 citation statements)

References 37 publications

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“…Several organic materials from animal origin, such as blood meal, hooves, horns, feathers, bones and manure have been evaluated as potential fertilizers [82]. [22] Chryseobacterium sediminis (µg) [72] Bacillus subtilis RSE163 (µM) [12] Bacillus subtilis RSE165 (µM) [ The use of feather hydrolyzate and keratinolytic bacteria having antagonistic and PGP activities could offer a number of economic and environmental advantages over chemicalbased as better organic fertilizer, biocontrol and biofertilizing agents. Organic amendments of N and/C rich feather hydrolyzate can serve the dual purpose of improving plant growth and stimulating beneficial microbial activity in soil.…”

Section: Discussionmentioning

confidence: 99%

“…produced 3 μg ml −1 of IAA in feather composting medium and 7 μg ml −1 of IAA when the medium was supplemented with 2% Trp . Chryseobacterium sediminis produced 44.4 μg ml −1 of IAA in absence of Trp, whereas, when the medium was supplemented with Trp the production increased to 64.26 μg ml −1 . Production of IAA is augmented with increase in the concentration of chicken feathers.…”

Section: Biofertilizing Potentialmentioning

confidence: 95%

“…Antifungal [52] Bacillus sp. Antinematodal [59] Bacillus sphaericus Insecticidal [60] Bacillus subtilis Antifungal IAA production P solubilization Ammonia production [57] Bacillus thuringiensis Insecticidal [60] Chryseobacterium sediminis IAA production [72] Stenotrophomonas maltophilia Antifungal IAA production Ammonia production [22] Streptomyces sp. Antifungal IAA production P solubilization Siderophore production Ammonia production [6] solubilizing bacteria (PSB) solubilize insoluble forms of P by releasing low molecular weight organic acids that is, acidification [77,78].…”

Section: Biofertilizing Potentialmentioning

confidence: 99%

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Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

et al. 2018

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Feathers account for 5–7% of the total weight of chicken have become one of the major pollutants due to their recalcitrant nature. Feather which is constituted of 90% keratin can be a good source of peptides, amino acids, and minerals for use as organic fertilizer. Traditional feather degradation methods consume large amount of energy and reduces the overall quality of the proteins. However, degradation of keratin by keratinolytic bacteria may represent as an alternative for the development of cheap, cost effective, eco‐friendly, and easily available nitrogen (N) and minerals rich source as potential organic fertilizers. Keratinase enzymes from bacteria are serine‐type proteases showing optimal activity at pH 6 to 9 and 30 to 50 °C. Mechanism of degradation includes, sulfitolysis, proteolysis, followed by deamination. Keratinolytic bacteria showing antagonism against important plant pathogens may act as biocontrol agent. Feather hydrolyzate can also be employed as nitrogenous fertilizers for plant growth. Tryptophan release from the feather degradation can act as precursor for plant phytohormone, indole‐3‐acetic acid (IAA). Solubilization of inorganic phosphate (P) by keratinolytic bacteria may further elevate the growth of plant. Application of hydrolyzate increases the water holding capacity, N, carbon (C) and mineral content of the soil. It elevates protein, amino acids, and chlorophyll content of plant. Feather hydrolyzate enhances seed germination and growth of plant. Soil application further increases the population of beneficial bacteria. The use of keratinolytic bacteria having antagonistic and plant growth promoting activities, and feather hydrolyzate can emerge as sustainable and alternative tools to promote and improve organic farming, agro‐ecosystem, environment, human health, and soil biological activities.

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Section: Discussionmentioning

confidence: 99%

Section: Biofertilizing Potentialmentioning

confidence: 95%

Section: Biofertilizing Potentialmentioning

confidence: 99%

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Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

et al. 2018

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“…The production of the enzymes was also high at 35 ° C for 24 hours. This is the genus of Arthrobacter and other positive keratinase bacteria, such as Bacillus subtilis and Bacillus pumilis (19), keratinase production temperature was shown to be 40 ° C in previous studies. In present study the optimized temperature for keratinase production was 37° C (Fig.…”

Section: Results and Discussion:-mentioning

confidence: 95%

Production and Optimization Studies of the Keratinase Enzyme by Bacillus Tequilensis MBR 25

Ramya¹,

Rao²

2020

IJAR

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Feather-degrading bacteria are the enzyme keratinase-degraded community of microorganisms that may degrade feathers. They are omnipresent in the food industry in feather waste. This food sector produces large quantities of feather waste that can hardly be treated using conventional chemical and physical methods. Due to the peculiar biochemical structure of the feather, mediated by beta-keratin and reinforced by several disulfide bonds, the degradation of feather waste is typically the most cost-effective and environmentally friendly process. The present study identified a medium for the development of keratinase by shaking flask culture (Bacillus tequilensis MBR 25). Feather (1%) and maximum keratinase efficiency have been determined for the highest keratinase output. Keratinase efficiency improved when all parameters were optimized simultaneously.

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“…In particular, waste feathers from poultry processing industry have been converted as peptone source for bacterial growth (Taskin and Kurbanoglu, 2011 3 ) and for development of culture media for production of mosquitocidal bacteria (Poopathi and Abidha, 2007 4 ). More detailed studies include the use of feather peptone for microbial production of carotenoids (Taskin et al 2011 5 ) and protein hydrolysates (Kshetri, 2017 6 ). Therefore, the results presented in this work are rather confirmatory, representing a restricted contribution to the field.…”

mentioning

confidence: 99%

Chicken feather hydrolysate as alternative peptone source for microbial cultivation

et al. 2019

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Background: Commercially available conventional growth media for the culture of microbes are expensive, hence the need for alternative cheaper sources. Livestock waste, in the form of feather and blood, are of value in biotechnology because of their high protein content. Hence the primary aim of this study was to produce a cheaper peptone alternative from chicken feather protein hydrolysate (CFPH) and blood meal (BM). Methods: The growth of selected bacteria and fungi was monitored in different media prepared from varied concentrations of peptone, CFPH and BM in order to determine the combination that produced maximum growth. Five different media, namely 100% peptone (control), 100% BM, 40% peptone + 60% CFPH, 40% BM + 60% CFPH and 20% peptone + 20% BM + 60% CFPH were prepared and used for the study. The different media were inoculated with 1 ml of each test organism ( Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus, Pseudomonas aeruginosa, Candida carpophila, Candida tropicalis and Pichia kundriavzevii) and their growth monitored for 10 h. Results: Pseudomonas aeruginosa, Proteus mirabilis and Staphylococcus aureus grew best in the 100% peptone, Klebsiella pneumoniae grew best in 100 BM. The fungi species were observed to grow best in 100% peptone. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of trichloroacetic acid (TCA), hydrochloric acid (HCl) and nitric acid (HNO 3) gave the best growth of E. coli. The 60% CFPH + 40% peptone combination (CFPH obtained with precipitate of TCA) also gave the best growth of C. tropicalis and Klebsiella pneumoniae. Conclusions: Overall, the 60% CFPH + 40% peptone combination showed the most potential as an alternative to peptone, especially for E. coli.

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Transforming Chicken Feather Waste into Feather Protein Hydrolysate Using a Newly Isolated Multifaceted Keratinolytic Bacterium Chryseobacterium sediminis RCM-SSR-7

Cited by 61 publications

References 37 publications

Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

Feather degradation by keratinolytic bacteria and biofertilizing potential for sustainable agricultural production

Production and Optimization Studies of the Keratinase Enzyme by Bacillus Tequilensis MBR 25

Chicken feather hydrolysate as alternative peptone source for microbial cultivation

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