In recent years, the incidence rate of gallstones has been increasing under the multiple influences of environment, heredity and diet. In the modern science and technology developed, treatment effect requirements are higher and higher. At present, no scar, less trauma, fast recovery of the NOTES surgery is more popular. Based on the understanding of NOTES, after searching the literature about gallbladder disease or clinical application, combined with related cases, this paper summarized the treatment measures, specific advantages and application cases of transgastric gallbladder conserving surgery. It has the advantages of no scar, less pain, faster recovery, fewer complications, shorter hospital stay and a series of important functions of gallbladder. At present, the key point of treatment is to select the most appropriate surgical method according to individual differences, physiological and genetic characteristics, disease characteristics, personal willingness and tolerance.