Transgenerational Effects on Calf Spermatogenesis and Metabolome Associated with Paternal Exposure to the Fukushima Nuclear Power Plant Accident

Suzuki, Banri; Tanaka, Shigefumi; Nishikawa, Koh-Ichi; Yoshida, Chikako; Yamada, Takahisa; Abe, Yasuyuki; Fukuda, T.; Kobayashi, Jin; Hayashi, Gosuke; Suzuki, Masatoshi; Urushihara, Yusuke; Koarai, Kazuma; Kino, Yasushi; Sekine, Tsutomu; Takahasi, Atsushi; Shimizu, Tomohito; Satoh, Hisashi; Saito, Kazuki; Isogai, Emiko; Kawasumi, Koh; Sugimura, Satoshi; Yamashiro, Hideaki; Fukumoto, Manabu

doi:10.1007/978-981-13-8218-5_10

Cited by 2 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acrosomal integrity of seven raccoon sperm samples was assessed by fluorescein isothiocyanate‐conjugated peanut agglutinin (FITC‐PNA) staining (Sigma‐Aldrich) as described previously (Suzuki et al, 2020) and categorised as follows: (a) intense acrosomal cap fluorescence indicating an intact acrosome; (b) acrosomal cap fluorescence indicating a partially damaged acrosome; and (c) no fluorescence indicating complete outer acrosomal membrane loss.…”

Section: Methodsmentioning

confidence: 99%

“…Following the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, large amounts of radioactive substances, particularly volatile elements, such as radioactive iodine, caesium and tellurium, were released into the environment. The ex‐evacuation zone comprises a 20‐km‐radius area around the FDNPP, from which we have established an archive system of livestock and wild animals (Ariyoshi et al., 2018; Fukuda et al., 2013; Fukuda et al., 2015; Fukuda et al., 2016; Koarai et al., 2016; Koarai et al., 2018; Koarai et al, 2020; Ohdaira et al., 2019; Suzuki et al, 2020; Takahashi et al., 2015; Takino et al., 2017; Urushihara et al., 2016; Meguro et al., 2019; Urushihara et al., 2018; Yamashiro et al., 2013; Yamashiro et al., 2014; Yamashiro et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (Procyon lotor)

Komatsu

Iwasaki

Murata

et al. 2021

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, we have established an archive system of livestock and wild animals from the surrounding ex‐evacuation zone. Wildlife within the alert zone have been exposed to low‐dose‐rate (LDR) radiation for a long continuous time. In this study, we analysed the morphological characteristics of the testes and in vitro fertilization (IVF) capacity of cryopreserved sperm of racoons from the ex‐evacuation zone of the FDNPP accident. The radioactivity of caesium‐137 (137Cs) was measured by gamma‐ray spectrometry, and the measured radioactivity concentration was 300–6,630 Bq/kg in the Fukushima raccoons. Notably, normal spermatogenesis was observed in the seminiferous tubules of the testes, with the germinal epithelium composed of a spermatogenic cell lineage with no evident ultrastructural alterations; freeze‐thawing sperm penetration ability was confirmed using the interspecific zona pellucida‐free mouse oocytes IVF assays. This study revealed that the chronic and LDR radiation exposure associated with the FDNPP accident had no adverse effect on the reproductive characteristics and functions of male raccoons.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (Procyon lotor)

Komatsu

Iwasaki

Murata

et al. 2021

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Radiation metabolomics has often been employed to diagnostically or therapeutically search for relevant biochemicals after exposure to ionizing radiation [ 46 , 47 , 48 , 49 , 50 ]. Metabolomics was used to investigate metabolite changes in rice seeds [ 15 , 18 ] and calf blood plasma [ 51 ] after the Fukushima nuclear accident. To demonstrate that the creeping wood sorrel responds to the low dose of radiation that is relevant in the field, we used contaminated soil collected from Fukushima as the radiation source.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Response of the Creeping Wood Sorrel Oxalis corniculata to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil

Sakauchi

Taira

Otaki

2021

Life

View full text Add to dashboard Cite

The biological consequences of the Fukushima nuclear accident have been intensively studied using the pale grass blue butterfly Zizeeria maha and its host plant, the creeping wood sorrel Oxalis corniculata. Here, we performed metabolomic analyses of Oxalis leaves from Okinawa to examine the plant metabolites that were upregulated or downregulated in response to low-dose radiation exposure from Fukushima’s contaminated soil. The cumulative dose of radiation to the plants was 5.7 mGy (34 μGy/h for 7 days). The GC-MS analysis revealed a systematic tendency of downregulation among the metabolites, some of which were annotated as caproic acid, nonanoic acid, azelaic acid, and oleic acid. Others were annotated as fructose, glucose, and citric acid, involved in the carbohydrate metabolic pathways. Notably, the peak annotated as lauric acid was upregulated. In contrast, the LC-MS analysis detected many upregulated metabolites, some of which were annotated as either antioxidants or stress-related chemicals involved in defense pathways. Among them, only three metabolite peaks had a single annotation, one of which was alfuzosin, an antagonist of the α1-adrenergic receptor. We conclude that this Oxalis plant responded metabolically to low-dose radiation exposure from Fukushima’s contaminated soil, which may mediate the ecological “field effects” of the developmental deterioration of butterflies in Fukushima.

show abstract

Transgenerational Effects on Calf Spermatogenesis and Metabolome Associated with Paternal Exposure to the Fukushima Nuclear Power Plant Accident

Cited by 2 publications

References 17 publications

Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (Procyon lotor)

Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (Procyon lotor)

Metabolomic Response of the Creeping Wood Sorrel Oxalis corniculata to Low-Dose Radiation Exposure from Fukushima’s Contaminated Soil

Contact Info

Product

Resources

About