Transgenic approaches for sterile insect control of dipteran livestock pests and lepidopteran crop pests.

Scott, M. J.; Morrison, Neil I.; Simmons, Gregory S.; Benedict, Mark Q.

doi:10.1079/9781780644516.0152

Cited by 8 publications

(10 citation statements)

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“…For the C. hominivorax SIT program this could be important if sterile flies are needed to both maintain the buffer zone near Colombia and to control an outbreak in any of the countries now free of the pest. The fluorescent marker could provide an easy means for distinguishing the released sterilized flies from wild type flies caught in the field 13 . Fertile males could be released if female-lethality is dominant.…”

Section: Discussionmentioning

confidence: 99%

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A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

Yan

Scott

2015

Sci Rep

112

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Genetic approaches, including the sterile insect technique (SIT), have previously been considered for control of the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. In an SIT program, females consume 50% of the diet but are ineffective as control agents and compete with females in the field for mating with sterile males, thereby decreasing the efficiency of the program. Consequently, transgenic sexing strains of L. cuprina were developed that produce 100% males when raised on diet that lacks tetracycline. However, as females die mostly at the pupal stage, rearing costs would not be significantly reduced. Here we report the development of transgenic embryonic sexing strains of L. cuprina. In these strains, the Lsbnk cellularization gene promoter drives high levels of expression of the tetracycline transactivator (tTA) in the early embryo. In the absence of tetracycline, tTA activates expression of the Lshid proapoptotic gene, leading to death of the embryo. Sex-specific RNA splicing of Lshid transcripts ensures that only female embryos die. Embryonic sexing strains were also made by combining the Lsbnk-tTA and tetO-Lshid components into a single gene construct, which will facilitate transfer of the technology to other major calliphorid livestock pests.

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Section: Discussionmentioning

confidence: 99%

“…An alternative to GSS is to employ transgenic technology to make conditional male-only strains 12 13 . Assembling transgenic sexing strains (TSS), requires a conditional gene expression system for controlling expression of a gene that is lethal to the insect.…”

mentioning

confidence: 99%

A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

Yan

Scott

2015

Sci Rep

112

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“…This technological advance is particularly interesting for the prospect of mass production of single-sex mosquito populations. Many field interventions against insect pests, including the sterile insect technique (SIT) ( Dyck et al 2005 ; Scott et al 2014 ), the release of insects carrying a dominant lethal (RIDL) ( Alphey et al 2014 ), or the prospective release of males carrying genes conferring resistance to Plasmodium , rely on mass production of purely male populations that this tool could facilitate. Any transgene carrying a fluorescent marker located on a sex chromosome can be exploited for single-sex population sorting ( Marois et al 2012 ; Bernardini et al 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Tools forAnopheles gambiaeTransgenesis

Volohonsky

Terenzi

Soichot

et al. 2015

G3 Genes|Genomes|Genetics

110

150

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Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest insects.

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“…The discussion surrounding the move from laboratory to field studies of an innovative vector control method is developing fast. [11][12][13] Gene drive strategies introduced through release of laboratory-reared mosquitoes or other vectors present additional questions, and this conversation is taking place in a public arena where many views are expressed. 14 The role of field studies such as MRR in building knowledge for pilot releases is well established.…”

Section: An Evolving Contextmentioning

confidence: 99%

Auditing Preparedness for Vector Control Field Studies

Collins

Quinlan

2020

The American Journal of Tropical Medicine and Hygiene

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The value of baseline entomological data to any future area-wide release campaign relies on the application of consistent methods to produce results comparable across different times and places in a stepwise progression to larger releases. Traditionally, standard operating procedures (SOPs) and operational plans support this consistency and, thus, the validity of emergent data. When release plans include transgenic mosquitoes for vector control or other novel beneficial insects, additional factors come into play such as biosafety permits, stakeholder acceptance, and ethics approval, which require even greater coordination and thoroughness. An audit approach was developed to verify the correct use of SOPs and appropriate performance of tasks during mosquito mark, release, recapture (MRR) studies. Audit questions matched SOPs, permit terms and conditions, and other key criteria, and can be used to support subsequent "spot check" verification by field teams. An external team of auditors, however, was found to be effective for initial checks in this example before the use of a transgenic strain of laboratory mosquitoes. We recommend similar approaches for field studies using release of novel beneficial insects, to ensure useful and valid data as an outcome and to support confidence in the rigor of the step-wise process. Similar objectives of regulatory compliance, enduring record keeping, and utility of studies were described for studies in containment facilities, 25 and an internal audit process was used to evaluate readiness for proceeding with key steps such as applications for import of novel organisms. 26

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Transgenic approaches for sterile insect control of dipteran livestock pests and lepidopteran crop pests.

Abstract: This chapter focuses on the various transgenic approaches in the control of dipteran livestock pests and lepidopteran crop pests based on the sterile insect technique.

Cited by 8 publications

References 0 publications

A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina

Tools forAnopheles gambiaeTransgenesis

Auditing Preparedness for Vector Control Field Studies

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