Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission

Byun, Sung June; Yuk, Seong‐Su; Jang, Yejin; Choi, Hye-Sun; Jeon, Mi-Hyang; Erdene-Ochir, TO; Kwon, Jung‐Hoon; Noh, Jin-Yong; Kim, Jeom Sun; Yoo, Jae Gyu; Song, Chang‐Seon

doi:10.1038/s41598-017-05270-8

Cited by 29 publications

(27 citation statements)

References 35 publications

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“…Although birds were not resistant to initial infection, viral transmission was prevented (Lyall et al, 2011 ). A different study demonstrated the possibility to suppress influenza A virus transmission in transgenic birds expressing the 3D8 single chain variable fragment (scFv), a gene that interacts with viral genome leading to suppression of viral shedding (June Byun et al, 2017 ).…”

Section: Generation Of Genetically Modified Chickensmentioning

confidence: 99%

Applications of Gene Editing in Chickens: A New Era Is on the Horizon

Sid

Schusser

2018

Front. Genet.

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The chicken represents a valuable model for research in the area of immunology, infectious diseases as well as developmental biology. Although it was the first livestock species to have its genome sequenced, there was no reverse genetic technology available to help understanding specific gene functions. Recently, homologous recombination was used to knockout the chicken immunoglobulin genes. Subsequent studies using immunoglobulin knockout birds helped to understand different aspects related to B cell development and antibody production. Furthermore, the latest advances in the field of genome editing including the CRISPR/Cas9 system allowed the introduction of site specific gene modifications in various animal species. Thus, it may provide a powerful tool for the generation of genetically modified chickens carrying resistance for certain pathogens. This was previously demonstrated by targeting the Trp38 region which was shown to be effective in the control of avian leukosis virus in chicken DF-1 cells. Herein we review the current and future prospects of gene editing and how it possibly contributes to the development of resistant chickens against infectious diseases.

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Section: Generation Of Genetically Modified Chickensmentioning

confidence: 99%

Applications of Gene Editing in Chickens: A New Era Is on the Horizon

Sid

Schusser

2018

Front. Genet.

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“…The viability of using a transgenic approach to protect against LPAI was subsequently addressed by Byun and colleagues [ 20 ]. In these studies, chicken were engineered to express the 3D8 single chain variable Fragment (scFv) customised to have an affinity for the IAV nuceloprotein.…”

Section: Genetically Modified Chickens Resistant To Avian Influenzmentioning

confidence: 99%

“…In these studies, chicken were engineered to express the 3D8 single chain variable Fragment (scFv) customised to have an affinity for the IAV nuceloprotein. scFv was of particular interest as it causes nuclease activity and has displayed hydrolytic activity against both RNA and DNA viruses [ 20 ]. Following direct infection with LPAI A/Korean native chicken/Korea/K040110/2010(H9N2), scFv transgenic chickens did not display any difference in oropharyngeal shedding when compared to their non-transgenic counterparts [ 20 ].…”

Section: Genetically Modified Chickens Resistant To Avian Influenzmentioning

confidence: 99%

Creating Disease Resistant Chickens: A Viable Solution to Avian Influenza?

Looi

Baker

Townson

et al. 2018

Viruses

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Influenza A virus (IAV) represents an ongoing threat to human and animal health worldwide. The generation of IAV-resistant chickens through genetic modification and/or selective breeding may help prevent viral spread. The feasibility of creating genetically modified birds has already been demonstrated with the insertion of transgenes that target IAV into the genomes of chickens. This approach has been met with some success in minimising the spread of IAV but has limitations in terms of its ability to prevent the emergence of disease. An alternate approach is the use of genetic engineering to improve host resistance by targeting the antiviral immune responses of poultry to IAV. Harnessing such resistance mechanisms in a “genetic restoration” approach may hold the greatest promise yet for generating disease resistant chickens. Continuing to identify genes associated with natural resistance in poultry provides the opportunity to identify new targets for genetic modification and/or selective breeding. However, as with any new technology, economic, societal, and legislative barriers will need to be overcome before we are likely to see commercialisation of genetically modified birds.

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“…As a result of a highly efficient genome modification capacity, these viral systems have also been used for avian transgenesis. After the first studies of transgenic animal production by retroviral infection in Eyal giladi and Kochav (EG&K) stage X chicken embryos, diverse transgenic birds have been successfully established for a variety of purposes, including functional protein expression and the development of model animals [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. Specifically, mass production of functional proteins using retroviral systems in serum and egg whites of transgenic chickens suggests its practical use as a bioreactor system, although the silencing of transgenes was observed in the subsequent generation (~5.6 mg/mL) [ 22 ].…”

Section: Development Of Transgenic Technologies and Application Fomentioning

confidence: 99%

Genome Modification Technologies and Their Applications in Avian Species

Lee

Kim

Ono

et al. 2017

IJMS

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The rapid development of genome modification technology has provided many great benefits in diverse areas of research and industry. Genome modification technologies have also been actively used in a variety of research areas and fields of industry in avian species. Transgenic technologies such as lentiviral systems and piggyBac transposition have been used to produce transgenic birds for diverse purposes. In recent years, newly developed programmable genome editing tools such as transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) have also been successfully adopted in avian systems with primordial germ cell (PGC)-mediated genome modification. These genome modification technologies are expected to be applied to practical uses beyond system development itself. The technologies could be used to enhance economic traits in poultry such as acquiring a disease resistance or producing functional proteins in eggs. Furthermore, novel avian models of human diseases or embryonic development could also be established for research purposes. In this review, we discuss diverse genome modification technologies used in avian species, and future applications of avian biotechnology.

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Transgenic Chickens Expressing the 3D8 Single Chain Variable Fragment Protein Suppress Avian Influenza Transmission

Cited by 29 publications

References 35 publications

Applications of Gene Editing in Chickens: A New Era Is on the Horizon

Applications of Gene Editing in Chickens: A New Era Is on the Horizon

Creating Disease Resistant Chickens: A Viable Solution to Avian Influenza?

Genome Modification Technologies and Their Applications in Avian Species

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