Transgenic Crops: Status, Potential, and Challenges

Mall, Tejinder; Han, Lei; Tagliani, Laura; Christensen, Cory A.

doi:10.1007/978-3-319-90650-8_16

Cited by 17 publications

(7 citation statements)

References 71 publications

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“…Inside the attL sites and adjacent to the target region, the sucrose-sensitive gene sacB [20] is linked to the ampicillin or gentamicin resistance gene (AmpR or GenR) and flanked by the pair of attR sites. Binary vector for accepting cargo on the first entry vector 1 An equal sign represents a recognition site of BsaI restriction enzyme. 2 Endentries with attL1/attL2 combination can also be constructed using other cloning methods such as TOPO cloning, traditional restriction enzyme and ligase method, and BP recombination [21] with commercially available vectors.…”

Section: Assembly Of Entry Vectorsmentioning

confidence: 99%

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An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants

Qin

Zhang

et al. 2022

Plants

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Transgenic technology can transfer favorable traits regardless of reproductive isolation and is an important method in plant synthetic biology and genetic improvement. Complex metabolic pathway modification and pyramiding breeding strategies often require the introduction of multiple genes at once, but the current vector assembly systems for constructing multigene expression cassettes are not completely satisfactory. In this study, a new in vitro gene stacking system, GuanNan Stacking (GNS), was developed. Through the introduction of Type IIS restriction enzyme-mediated Golden Gate cloning, GNS allows the modular, standardized assembly of target gene expression cassettes. Because of the introduction of Gateway recombination, GNS facilitates the cloning of superlarge transgene expression cassettes, allows multiple expression cassettes to be efficiently assembled in a binary vector simultaneously, and is compatible with the Cre enzyme-mediated marker deletion mechanism. The linked dual positive-negative marker selection strategy ensures the efficient acquisition of target recombinant plasmids without prokaryotic selection markers in the T-DNA region. The host-independent negative selection marker combined with the TAC backbone ensures the cloning and transfer of large T-DNAs (>100 kb). Using the GNS system, we constructed a binary vector containing five foreign gene expression cassettes and obtained transgenic rice carrying the target traits, proving that the method developed in this research is a powerful tool for plant metabolic engineering and compound trait transgenic breeding.

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Section: Assembly Of Entry Vectorsmentioning

confidence: 99%

“…Transgene stacking is the method of combining two or more foreign genes in the same plant. Genetically modified crop varieties with stacked traits can meet wider and more complex user needs and show more commercial value than single-trait varieties [1].…”

Section: Introductionmentioning

confidence: 99%

An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants

Qin

Zhang

et al. 2022

Plants

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“…HIGS is a common method for increasing plant tolerance to pests and pathogens (Ghag, 2017). Some RNAibased genetically modified crops, such as DuPont's Plenish R high-oleic acid soybean (Mall et al, 2018) and Monsanto's SmartStax Pro to eradicate western corn earworm (Ludwick et al, 2017), have recently been approved for commercialization. HIGS also ensures that dsRNA/siRNA molecules generated by the host plants are continuously spread to biotrophic pathogens such as PPNs (Essigmann et al, 2012;Jain et al, 2018).…”

Section: Classical Breeding Techniques To Modern Toolsmentioning

confidence: 99%

Breeding for Root-Knot Nematode Resistance in Eggplant: Progress and Prospects

Sharma¹,

Kaushik²

2021

Preprint

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Eggplant is a functional food owing to its anti-diabetic, anti-inflammatory, and cardio-protective properties. Root-knot nematodes (RKN) are a threat to the successful production of eggplant. RKN infestation manifests as root damage, stunted development, and structural deformations of the plant. RKN infestation can be managed using a variety of management techniques like soil amendments and chemical treatments. Breeding for nematode tolerance is critical for high yields and stable results. As a result, breeding approaches are the most efficient and cost-effective nematode management methods. Furthermore, with advances in breeding technology and genomics assistance, it is becoming more feasible and straightforward. As a result, we've compiled a list of the most recent breeding developments for Meloidogyne spp. resistance in eggplant. We hope this information will serve as an important resource for the eggplant breeders.

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“…In the coming decades, the growing demand for oilseeds can be achieved by using advanced molecular breeding techniques such as complementary breeding tools, which would be very useful to accelerate all crop improvement programs to produce climate-resilient crops. While transgenic approaches have so far been successfully used in oilseeds to improve a wide range of traits (Meesapyodsuk et al, 2018 ; Na et al, 2018 ; Shah et al, 2018 ; Kim et al, 2019 ; Wang et al, 2019 ), only a small number of these devices have made it to the market due to poor public perception as well as the disproportionately high cost and length of existing regulatory processes (Mall et al, 2018 ). Therefore, in this review, we aim to analyze recent results on the response and tolerance to heat stress at the cell, organelle, and whole plant level and describe the numerous approaches used to increase heat tolerance in oilseed crops.…”

Section: Introductionmentioning

confidence: 99%

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

et al. 2021

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Temperature is one of the decisive environmental factors that is projected to increase by 1. 5°C over the next two decades due to climate change that may affect various agronomic characteristics, such as biomass production, phenology and physiology, and yield-contributing traits in oilseed crops. Oilseed crops such as soybean, sunflower, canola, peanut, cottonseed, coconut, palm oil, sesame, safflower, olive etc., are widely grown. Specific importance is the vulnerability of oil synthesis in these crops against the rise in climatic temperature, threatening the stability of yield and quality. The natural defense system in these crops cannot withstand the harmful impacts of heat stress, thus causing a considerable loss in seed and oil yield. Therefore, a proper understanding of underlying mechanisms of genotype-environment interactions that could affect oil synthesis pathways is a prime requirement in developing stable cultivars. Heat stress tolerance is a complex quantitative trait controlled by many genes and is challenging to study and characterize. However, heat tolerance studies to date have pointed to several sophisticated mechanisms to deal with the stress of high temperatures, including hormonal signaling pathways for sensing heat stimuli and acquiring tolerance to heat stress, maintaining membrane integrity, production of heat shock proteins (HSPs), removal of reactive oxygen species (ROS), assembly of antioxidants, accumulation of compatible solutes, modified gene expression to enable changes, intelligent agricultural technologies, and several other agronomic techniques for thriving and surviving. Manipulation of multiple genes responsible for thermo-tolerance and exploring their high expressions greatly impacts their potential application using CRISPR/Cas genome editing and OMICS technology. This review highlights the latest outcomes on the response and tolerance to heat stress at the cellular, organelle, and whole plant levels describing numerous approaches applied to enhance thermos-tolerance in oilseed crops. We are attempting to critically analyze the scattered existing approaches to temperature tolerance used in oilseeds as a whole, work toward extending studies into the field, and provide researchers and related parties with useful information to streamline their breeding programs so that they can seek new avenues and develop guidelines that will greatly enhance ongoing efforts to establish heat stress tolerance in oilseeds.

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Transgenic Crops: Status, Potential, and Challenges

Cited by 17 publications

References 71 publications

An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants

An Efficient Modular Gateway Recombinase-Based Gene Stacking System for Generating Multi-Trait Transgenic Plants

<strong>Breeding for </strong><strong>Root-Knot Nematode </strong><strong>Resistance in Eggplant: Progress and Prospects</strong>

Adaptation Strategies to Improve the Resistance of Oilseed Crops to Heat Stress Under a Changing Climate: An Overview

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