1992
DOI: 10.1105/tpc.4.12.1495
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Transgenic maize plants by tissue electroporation.

Abstract: In this paper, we describe the transformation of regenerable maize tissues by electroporation. In many maize lines, immature zygotic embryos can give rise to embryogenic callus cultures from which plants can be regenerated. Immature zygotic embryos or embryogenic type I calli were wounded either enzymatically or mechanically and subsequently electroporated with a chimeric gene encoding neomycin phosphotransferase (neo). Transformed embryogenic calli were selected from electroporated tissues on kanamycin-contai… Show more

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Cited by 219 publications
(69 citation statements)
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“…These partial detachments of the cell wall from the membrane cause a void space between the rigid cell wall and the cell membrane and enables the required contact between the cell membrane and the macromolecule that is being introduced into the cytoplasm (Fig. 5) (D'Halluin et al 1992;Ganeva et al 1995;Sabri et al 1996a;Eynard et al 1997;Wu and Feng 1999).…”
Section: Osmotic Pressurementioning
confidence: 99%
See 1 more Smart Citation
“…These partial detachments of the cell wall from the membrane cause a void space between the rigid cell wall and the cell membrane and enables the required contact between the cell membrane and the macromolecule that is being introduced into the cytoplasm (Fig. 5) (D'Halluin et al 1992;Ganeva et al 1995;Sabri et al 1996a;Eynard et al 1997;Wu and Feng 1999).…”
Section: Osmotic Pressurementioning
confidence: 99%
“…Even though the cell wall represents a barrier, osmotic shock pretreatment that provokes plasmolysis can be used to create a passage of molecules through the cell wall (D'Halluin et al 1992;Ganeva et al 1995;Sabri et al 1996a;Eynard et al 1997;Wu and Feng 1999).…”
Section: Transfection Of Intact Plant Tissuementioning
confidence: 99%
“…Maize (Zea mays) inbred lines A188 (Green and Phillips, 1975) and H99 (D'Halluin et al, 1992) and transgenic lines were grown under standard greenhouse conditions at 26°C with 16 hours light and a relative air humidity of ~60%. Cells of the maize embryo sac before fertilization were isolated according to Kranz et al (Kranz et al, 1991) and after fertilization according to Cordts et al (Cordts et al, 2001) with the exception that ears were kept on wet paper instead of MS medium.…”
Section: Plant Growth Isolation Of Cells From the Female Gametophytementioning
confidence: 99%
“…Maize (Zea mays) inbred lines A188 (Green and Phillips, 1975) and H99 (D'Halluin et al, 1992) as well as transgenic lines were grown under standard greenhouse conditions at 26°C with 16 h light and a relative air humidity of about 60%. Cells of the maize embryo sac were isolated according to Kranz et al (1991) and zygotes according to Cordts et al (2001).…”
Section: Plant Material Isolation Of Cells From the Female Gametophymentioning
confidence: 99%