Transgenic mice producing the trans 10, cis 12-conjugated linoleic acid present reduced adiposity and increased thermogenesis and fibroblast growth factor 21 (FGF21)

Trans 10, cis 12-conjugated linoleic acid reduced reproductive ability by disrupting the estrus cycle in female mice

“…These results proved that Pai was successfully inserted and appropriately functioned in the tg ovaries. Moreover, the gas chromatography of adult tg mice demonstrated that the Pai protein successfully converted LA into t10c12-CLA, and fatty acid contents had undergone changes ( Rao et al, 2023a ). The result predicted that the 10c12-CLA produced by Pai altered the serum fatty acid contents of tg mice.…”

“…The C57BL/6J mice were obtained from the Laboratory Animal Centre, Yangzhou University, China, and used as wild-type (wt) mice. The tg mice (C57BL/6J background) were produced by our lab ( Rao et al, 2023a , b ). Each mouse per cage used in this study was maintained in an environment with a controlled temperature of 22 ± 2 °C, with artificial light cycles (12 h:12 h, lights on at 07:00), and was fed ad libitum with a standard diet containing 10% kcal% fat.…”

“…The ovaries were fixed in 4% paraformaldehyde overnight, dehydrated by a graded alcohol series, embedded in paraffin, and then serially sectioned (5 µm) using standard procedures. Then, the sections were stained with Haematoxylin and Eosin (HE) solution and examined under a light microscope (BX43, Olympus, Japan) as previously described ( Rao et al, 2023a ). The ovaries were serially sectioned, and one-fifth to tenth sections were counted for the follicle quantification.…”

“…Propionibacterium acnes isomerase (Pai) could successfully convert linoleic acid (LA) into t10c12-CLA in recombinant lactic acid bacteria and murine 3T3 cells ( Li et al, 2015 ; Wang et al, 2021 ). Then, a novel transgenic mouse was produced in our lab by introducing the Pai-expressing cassette into the Rosa26 locus of C57BL/6 mice using CRISPR-Cas9 technology ( Rao et al, 2023a , b ). The transgenic (tg) mouse could synthesize t10c12-CLA by itself.…”

“…The transgenic (tg) mouse could synthesize t10c12-CLA by itself. The male mouse could produce t10c12-CLA in various tissues throughout the body while increasing heat production and decreasing fat accumulation ( Rao et al, 2023a ). This is consistent with the anti-obesity results from oral t10c12-CLA administration, suggesting that the mouse can be used to investigate the impact of t10c12-CLA in mice.…”