Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea

Schestibratov, K.A.; Dolgov, Sergey

doi:10.1016/j.scienta.2005.03.016

Cited by 81 publications

(42 citation statements)

References 37 publications

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“…A transformation frequency of 0.95% has been reported for the cultivar "Rapella" 62 and 6.5% for "Red Coat" 30,31 and an efficiency of 4.22% and 10% with cultivar Chandeler 22,60 and 11% for "Firework." 63 …”

Section: Plant Transformationmentioning

confidence: 99%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome, which makes the breeding of new cultivars difficult. simple and efficient methods for transformation and regeneration are required for cultivars improvement in strawberries. In the present study, adventitious shoot regeneration has been investigated in three cultivated strawberry plants, i.e., Festival, sweet charlie, and Florida via direct organogenesis using the in vitro juvenile leaves as explants. explants were collected after sub-culturing on a propagation medium composed of Ms supplemented with 0.5 mg/l Ba; 0.1 mg/l Ga3 and 0.1 mg/l IBa. To select the suitable organogenesis, the explants of the three cultivars were cultured on Ms medium supplemented with different concentrations of TDZ (1, 2, 3, and 4 mg/l), then incubated at a temperature of 22 °c ± 2. Medium containing 2 mg/l TDZ revealed the best regeneration efficiency with the three cultivars (72% for Festival and 73% for sweet charlie and Florida). after 4 weeks the produced shoots were cultured on Ms medium with different concentrations of Ba and Kin to enhance shoot elongation. Results showed that the medium containing 1.5 mg/l Ba and 0.5 mg/l Kin revealed highest elongation efficiency (88% and 94%) for Festival and sweet charlie, respectively. On the other hand, medium media containing 1.5 mg/l Ba and 0.1 mg/l Kin showed highest elongation efficiency (90%) in Florida. elongated shoots were successfully rooted on Ms medium containing 1.5 mg/l Naa. Furthermore, transformation of the two cultivars, Festival and sweet charlie, has been established via Agrobacterium strain LBa44404 containing the plasmid pIsV2678 with gus-intron and bar genes. Three days post-cocultivation, GUs activity was screened using the histochemical assay. The results showed 16% and 18% of the tested plant materials had changed into a blue color for Festival and sweet charlie, respectively. Out of 120 explants only 13 shoots were developed on bialaphos medium for each cultivar, representing 10.8% bialaphos-resistant strawberry shoot. The presence of both the genes bar and uid a was detected by PcR and Northern, giving a transformation efficiency of 5%.

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Section: Plant Transformationmentioning

confidence: 99%

Improved regeneration and transformation protocols for three strawberry cultivars

et al. 2013

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“…Both antibiotics have been used in strawberry transformation, and a variety of concentrations have been shown to be effective depending on the cultivar; kanamycin has been used frequently at the concentrations of 50–70 mg/l [10,32-35] and 25 mg/l [5,7,9]. However, Houde et al .…”

Section: Resultsmentioning

confidence: 99%

“…During the past few years several reports on improving transgenic strawberry production methods have been published [4-6], and plants e.g. with better fruit firmness [7], freezing tolerance [8] and enhanced resistance to gray mold fungus [9,10] have been achieved. All the reported procedures are usually fine-tuned for certain varieties.…”

Section: Introductionmentioning

confidence: 99%

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Hanhineva

Kärenlampi

2007

BMC Biotechnol

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Background: Strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome which complicates the breeding of new cultivars. For certain traits, genetic engineering offers a potential alternative to traditional breeding. However, many strawberry varieties are quite recalcitrant for Agrobacterium-mediated transformation, and a method allowing easy handling of large amounts of starting material is needed. Also the genotyping of putative transformants is challenging since the isolation of DNA for Southern analysis is difficult due to the high amount of phenolic compounds and polysaccharides that complicate efficient extraction of digestable DNA. There is thus a need to apply a screening method that is sensitive and unambiguous in identifying the different transformation events.

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“…These PR-proteins have been classified into 17 families based on their primary structures, immunologic relationships, and enzymatic properties including chitinases (PR-3, -4, -8, -11), β-1, 3-glucanases (PR-2), proteinase inhibitors (PR-6), and peroxidases (PR-9) 5 . Introduction of PR-genes has been successfully used to improve disease resistance in some plant species 6,7 . Studies of plant-pathogen interactions have mostly focused on PR-proteins and other proteins involved in defence mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Wongpia¹,

Lomthaisong

2010

ScienceAsia

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Wilt disease caused by Fusarium oxysporum f. sp. capsici is a major problem of chilli pepper production worldwide that calls for a better understanding of defensive mechanisms in the chilli plant. We used a proteomic technique to investigate protein responses of chilli pepper to F. oxysporum f. sp. capsici. Two cultivars of resistant (Mae Ping 80) and susceptible (Long Chilli 455) plants were cultured in vitro. Chilli plants at 6-week growth were then infected with a suspension of F. oxysporum f. sp. capsici or distilled water used as a control. After 48 h of infection, proteins were extracted and analysed using 2DE to identify the responsive proteins. At least 9 spots were differentially expressed in the resistant cultivar (5 increasing, 4 decreasing) and 1 supplementary; while 15 increasing, 11 decreasing, and 11 supplementary protein spots were found in the susceptible cultivar. These proteins were then identified by MALDI-TOF MS combined with bioinformatics methods. Some of the induced proteins e.g., NADPH HC toxin reductase, serine/threonine protein kinase, and 1-aminocyclopropane-1-carboxylate synthase 3 are involved in plant defence mechanism. In order to determine the Fusarium wilt protective proteins in chilli plant, the protein patterns of healthy resistance were compared with those of susceptible cultivars. Interestingly, resistance showed higher expression of proteins related to ROS detoxification. Moreover, the ability of chilli plant to resist Fusarium wilt disease was related to the expression of non-inducible immunity 1 protein.

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Transgenic strawberry plants expressing a thaumatin II gene demonstrate enhanced resistance to Botrytis cinerea

Cited by 81 publications

References 37 publications

Improved regeneration and transformation protocols for three strawberry cultivars

Improved regeneration and transformation protocols for three strawberry cultivars

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