Transient embolization with microspheres of polyhydroxyalkanoate renders efficient adenoviral transduction of pancreatic capillary<i>in vivo</i>

Zhang, Shuili; Zheng, Dejin; Fan, Wen-zhu; Wei, Daixu; Peng, Siwu; Tang, Mimi L.K.; Chen, Guoqiang; Wei, Chiju

doi:10.1002/jgm.2650

Cited by 7 publications

(5 citation statements)

References 53 publications

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“…Islet cells were labeled occasionally, whereas labeling of the acinar and ductal tissues was barely detectable. Efficient pancreatic capillary targeting by using PHBHHx microsphere-adenoviral complexes was established for the treatment of capillaryrelated diseases [63].…”

Section: Applications As Drug Delivery Matricesmentioning

confidence: 99%

Polyhydroxyalkanoates (PHA) for therapeutic applications

Zhang

Shishatskaya

Volova

et al. 2018

Materials Science and Engineering: C

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197

106

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As intracellular carbon and energy storage materials, polyhydroxyalkanoates (PHA) are a diverse biopolyesters synthesized by many bacteria. PHA have been produced in large quantity for various application research including medical implants for approximately 30years. Many studies demonstrated that PHA are promising implant materials due to their diverse and ascendant mechanical, biodegradable and tissue compatible properties. Importantly, common PHA biodegradation products including oligomers and monomers are also not toxic to the cells and tissues. Pharmaceutical applications of some PHA degradation products also have been reported. So far, no study has been reported to have any carcinogenesis result induced by any PHA or their biodegradation products. All results suggest that PHA could be developed into various bio-implant products.

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Section: Applications As Drug Delivery Matricesmentioning

confidence: 99%

Polyhydroxyalkanoates (PHA) for therapeutic applications

Zhang

Shishatskaya

Volova

et al. 2018

Materials Science and Engineering: C

Self Cite

197

106

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“…And local injection is a perfect option for this drug dosage control ( Shishatskaya et al, 2008 ). High encapsulation efficiency and sustainable release of antibiotics, such as tetracycline and gentamicin, deposited in PHA microspheres could also be achieved with therapeutic effects in corresponded disease models ( Sendil et al, 1999 ; Zhang et al, 2012 ). For example, Zhang et al prepared PHBHHx-based microspheres with a capillary-size diameter of 5–10 μm, carrying adenoviruses fused with a green fluorescence protein for therapeutic study ( Zhang et al, 2012 ).…”

Section: Fabrication and Applications Of Pha Particlesmentioning

confidence: 99%

“…Five days after the surgery, approximately 40% of endothelial cells in the pancreas were labeled, while the other cells in the islet were merely detectable. Therefore, this efficient pancreatic capillary targeting implant based on PHA microsphere-adenoviral complexes are of great potential and values in capillary-related diseases treatment in the coming future ( Zhang et al, 2012 ).…”

Section: Fabrication and Applications Of Pha Particlesmentioning

confidence: 99%

A Polyhydroxyalkanoates-Based Carrier Platform of Bioactive Substances for Therapeutic Applications

Zhang

Liu

Hao

et al. 2022

Front. Bioeng. Biotechnol.

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Bioactive substances (BAS), such as small molecule drugs, proteins, RNA, cells, etc., play a vital role in many therapeutic applications, especially in tissue repair and regeneration. However, the therapeutic effect is still a challenge due to the uncontrollable release and instable physico-chemical properties of bioactive components. To address this, many biodegradable carrier systems of micro-nano structures have been rapidly developed based on different biocompatible polymers including polyhydroxyalkanoates (PHA), the microbial synthesized polyesters, to provide load protection and controlled-release of BAS. We herein highlight the developments of PHA-based carrier systems in recent therapeutic studies, and give an overview of its prospective applications in various disease treatments. Specifically, the biosynthesis and material properties of diverse PHA polymers, designs and fabrication of micro- and nano-structure PHA particles, as well as therapeutic studies based on PHA particles, are summarized to give a comprehensive landscape of PHA-based BAS carriers and applications thereof. Moreover, recent efforts focusing on novel-type BAS nano-carriers, the functionalized self-assembled PHA granules in vivo, was discussed in this review, proposing the underlying innovations of designs and fabrications of PHA-based BAS carriers powered by synthetic biology. This review outlines a promising and applicable BAS carrier platform of novelty based on PHA particles for different medical uses.

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“…In addition, PHBVHHx solid microspheres (SMS) and hollow microspheres (HMSs) were prepared by a typical oil-in-water (O/W) simple emulsion method [12] and water-in-oil-in-water (W1/O/W2) double emulsion method [9], respectively. For SMS, 15 ml of the aforementioned PHBVHHx/methylene chloride solution (phase O) was fed drop wise into 100 ml 0.1 wt% PVA solution under magnetic stirring at 400 rpm to form the emulsion (O/W2).…”

Section: Preparations Of Phbvhhx Microspheresmentioning

confidence: 99%

“…PHBVHHx also exhibited better proliferation of mesenchymal stem cells (MSCs) and osteogenic differentiation when compared with other PHA and PLA [4]. Since other similar PHA have been used to prepare smooth/rough-surface microspheres as cells or drugs carriers [7][8][9][10][11][12], PHBVHHx had not yet been used for the same purposes.…”

Section: Introductionmentioning

confidence: 99%

Suspended polyhydroxyalkanoate microspheres as 3D carriers for mammalian cell growth

Wei

Dao

Liu

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Different forms of biopolyester PHBVHHx microspheres were prepared so as to compare the mammalian cell behaviors in suspension cultivation system. Based on a microbial terpolyester PHBVHHx consisting of 3-hydroxybutyrate (HB), 3-hydroxyvalerate (HV), and 3-hydroxyhexanoate (HHx), solid microspheres (SMSs), hollow microspheres (HMSs), and porous microspheres (PMS) were successfully prepared by a modified solvent evaporation method involving gas-in-oil-in-water (G1/O/W2) double emulsion, water-in-oil-in-water (W1/O/W2) double emulsion and oil-in-water (O/W) single emulsion, respectively. Generally, PMSs have diameters ranging from 330 to 400 μm with pore sizes of 10 to 60 μm. The pores inside the PMSs were found well interconnected compared with PHBVHHx prepared by the traditional solvent evaporation method, resulting in the highest water uptake ratio. When inoculated with human osteoblast-like cells lasting 6 days, PMS showed much better cell attachment and proliferation compared with other less porous microspheres due to its large inner space as a 3 D carrier. Cell migration towards surface and other interconnected inner pores was clearly observable. Dead or apoptotic cells were found more common among less porous SMSs or HMSs compared with highly porous PMSs. It is therefore concluded that porous PHBVHHx microspheres with larger surface open pores and interconnected inner pores can serve as a carrier or scaffold supporting more and better cell growth for either injectable purposes or simply supporting cell growth.

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Transient embolization with microspheres of polyhydroxyalkanoate renders efficient adenoviral transduction of pancreatic capillaryin vivo

Abstract: The present study has established an approach for efficient pancreatic capillary targeting by using microsphere-adenoviral complexes. This procedure could be invaluable for the treatment of capillary-related diseases.

Cited by 7 publications

References 53 publications

Polyhydroxyalkanoates (PHA) for therapeutic applications

Polyhydroxyalkanoates (PHA) for therapeutic applications

A Polyhydroxyalkanoates-Based Carrier Platform of Bioactive Substances for Therapeutic Applications

Suspended polyhydroxyalkanoate microspheres as 3D carriers for mammalian cell growth

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