Transient exposure to sublethal concentrations of a pesticide mixture (chlorpyrifos–difenoconazole) caused different responses in fish species from different trophic levels of the same community

Redondo-López, Sergei; León, Ana C.; Jiménez, Katherine; Solano, Karla; Blanco-Peña, Kinndle; Mena, Freylan

doi:10.1016/j.cbpc.2021.109208

Cited by 8 publications

(4 citation statements)

References 72 publications

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“…According to Melvin and Wilson (2013) and Peterson et al (2017), behavioral endpoints are generally 10-1000 times more sensitive compared to classic endpoints, such as mortality. Some of our results on the behavior of common carp exposed to both pesticides are in agreement with Bonansea et al (2016), Redondo-López et al (2022), Thoré et al (2021a), and Özok et al (2018 who reported similar changes. All the behavioral traits were affected by the pesticides throughout the experiment, and behavioral responses became more distinct with increasing pesticide concentrations.…”

Section: Behaviorsupporting

confidence: 92%

Assessment of the Exposure of Two Pesticides on Common Carp (Cyprinus Carpio Linnaeus, 1758): Are the Prolonged Biomarker Responses Adaptive or Destructive?

Yancheva,

Georgieva,

Velcheva

et al. 2022

SSRN Journal

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Section: Behaviorsupporting

confidence: 92%

Assessment of the Exposure of Two Pesticides on Common Carp (Cyprinus Carpio Linnaeus, 1758): Are the Prolonged Biomarker Responses Adaptive or Destructive?

Yancheva,

Georgieva,

Velcheva

et al. 2022

SSRN Journal

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“…Ellman’s assay [ 7 ] is the gold standard for the determination of cholinesterase activity in botany, ecotoxicology, military toxicology, and drug control [ 8 , 9 , 10 ]. However, alternative substrates for cholinesterase determination exist.…”

Section: Introductionmentioning

confidence: 99%

Open Meter Duo: Low-Cost Instrument for Fluorimetric Determination of Cholinesterase Activity

Keresteš,

Mozo,

Pohanka

2024

Sensors

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Environmental screening is essential due to the increased occurrence of harmful substances in the environment. Open Meter Duo (OMD) is an open-source field photo/fluorimeter that uses an RGB diode that imitates a color according to the selected wavelength and uses a UV LED from the security kit diode as an excitation light source. The prepared PCB shield with a 3D-printed aperture was connected to Arduino UNO R4 WiFi. This system was used for the fluorescent detection of cholinesterase activity with the indoxyl acetate method. Carbofuran—a toxic pesticide—and donepezil—a drug used to treat Alzheimer’s disease—were tested as model inhibitors of cholinesterase activity. The limit of detection of indoxyl acetate was 11.6 μM, and the IC50 values of the inhibitors were evaluated. This system is optimized for wireless use in field analysis with added cloud support and power source. The time of analysis was 5 min for the fluorimetric assay and 20 min for the optional photometric assay. The time of field operation was approximately 4 h of continuous measurement. This system is ready to be used as a cheap and easy control platform for portable use in drug control and point-of-care testing.

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“…Due to the potential for antagonistic or even synergistic effects, contaminants from different chemical groups and their interactions are now a primary focus. Animals frequently exhibit different biochemical responses when exposed to the same pesticides in a mixture of pesticides [ 15 ]. This is a worrisome finding because when pesticides enter natural reservoirs, they meet other chemicals, can interact with each other, and aggravate final adverse outcomes due to cocktail effects.…”

Section: Introductionmentioning

confidence: 99%

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

Khatib

Horyn

Боднар

et al. 2023

Animals

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Our research sought to determine the molecular and biochemical effects of environmentally relevant exposure to commonly used chloro-s-triazine herbicide terbuthylazine and organophosphate insecticide malathion on zebrafish. To this aim, mature zebrafish were exposed to 2 and 30 µg L−1 terbuthylazine and 5 and 50 µg L−1 malathion alone and in combination for 14 days. Aside from the accumulation of TBARS and protein carbonyls, a decrease in antioxidants and succinate dehydrogenase activity, an increase in oxidized glutathione, and enhanced apoptosis via Caspase-3 and BAX overexpression were observed. Furthermore, terbuthylazine and malathion induced mitochondrial swelling (up to 210% after single exposure and up to 470% after co-exposure) and lactate dehydrogenase leakage (up to 268% after single exposure and up to 570% after co-exposure) in a concentration-dependent manner. Significant upregulation of ubiquitin expression and increased cathepsin D activity were characteristics that appeared only upon terbuthylazine exposure, whereas the induction of IgM was identified as the specific characteristic of malathion toxicity. Meanwhile, no alterations in the zebrafish hypothalamic-pituitary-thyroid axis was observed. Co-exposure increased the adverse effects of individual pesticides on zebrafish. This study should improve the understanding of the mechanisms of pesticide toxicity that lead to fish impairment and biodiversity decline.

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Transient exposure to sublethal concentrations of a pesticide mixture (chlorpyrifos–difenoconazole) caused different responses in fish species from different trophic levels of the same community

Cited by 8 publications

References 72 publications

Assessment of the Exposure of Two Pesticides on Common Carp (Cyprinus Carpio Linnaeus, 1758): Are the Prolonged Biomarker Responses Adaptive or Destructive?

Assessment of the Exposure of Two Pesticides on Common Carp (Cyprinus Carpio Linnaeus, 1758): Are the Prolonged Biomarker Responses Adaptive or Destructive?

Open Meter Duo: Low-Cost Instrument for Fluorimetric Determination of Cholinesterase Activity

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

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