Transient expression of simple epithelial keratins by mesenchymal cells of regenerating newt limb

Ferretti, Patrizia; Fekete, Donna M.; Patterson, Michaela; Lane, E B

doi:10.1016/0012-1606(89)90045-6

Cited by 54 publications

(46 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its expression appears to be nerve‐dependent, because it is not expressed in the limb bud or in regenerating aneurogenic limbs (Fekete & Brockes, 1988; Ferretti & Brockes, 1991; Gordon & Brockes, 1988). Three keratins, keratins 8 and 18 and a newt type II keratin, NvKII, are expressed in newt blastema cells (Ferretti, Brockes, & Brown, 1991; Ferretti, Fekete, Patterson, & Lane, 1989). NvKII and 9G1 are also expressed in the AEC of the newt limb blastema.…”

Section: Formation Of the Accumulation Blastemamentioning

confidence: 99%

Mechanisms of urodele limb regeneration

Stocum

2017

Regeneration

109

View full text Add to dashboard Cite

This review explores the historical and current state of our knowledge about urodele limb regeneration. Topics discussed are (1) blastema formation by the proteolytic histolysis of limb tissues to release resident stem cells and mononucleate cells that undergo dedifferentiation, cell cycle entry and accumulation under the apical epidermal cap. (2) The origin, phenotypic memory, and positional memory of blastema cells. (3) The role played by macrophages in the early events of regeneration. (4) The role of neural and AEC factors and interaction between blastema cells in mitosis and distalization. (5) Models of pattern formation based on the results of axial reversal experiments, experiments on the regeneration of half and double half limbs, and experiments using retinoic acid to alter positional identity of blastema cells. (6) Possible mechanisms of distalization during normal and intercalary regeneration. (7) Is pattern formation is a self‐organizing property of the blastema or dictated by chemical signals from adjacent tissues? (8) What is the future for regenerating a human limb?

show abstract

Section: Formation Of the Accumulation Blastemamentioning

confidence: 99%

Mechanisms of urodele limb regeneration

Stocum

2017

Regeneration

109

View full text Add to dashboard Cite

show abstract

“…Tazaki et al looked for genes upregulated during both tail and limb regeneration using a macroarray (Tazaki et al, 2005). A total of 15 genes were identified as being in common, including keratin 8, which had already been identified as differentially regulated during newt limb regeneration (Ferretti et al, 1989). When Tazaki's list of tail regeneration is compared with limb regeneration data from Pearl (Pearl et al, 2008), using unigene identifiers, a few genes can be identified as common to both lists (C.W.B., unpublished observations).…”

Section: Can We Identify Common Mechanisms and Genes?mentioning

confidence: 99%

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Beck

Belmonte

Christen

2009

Developmental Dynamics

150

139

View full text Add to dashboard Cite

While Xenopus is a well-known model system for early vertebrate development, in recent years, it has also emerged as a leading model for regeneration research. As an anuran amphibian, Xenopus laevis can regenerate the larval tail and limb by means of the formation of a proliferating blastema, the lens of the eye by transdifferentiation of nearby tissues, and also exhibits a partial regeneration of the postmetamorphic froglet forelimb. With the availability of inducible transgenic techniques for Xenopus, recent experiments are beginning to address the functional role of genes in the process of regeneration. The use of soluble inhibitors has also been very successful in this model. Using the more traditional advantages of Xenopus, others are providing important lineage data on the origin of the cells that make up the tissues of the regenerate. Finally, transcriptome analyses of regenerating tissues seek to identify the genes and cellular processes that enable successful regeneration.

show abstract

“…However, in amphibians, such keratin gene expression patterns have not been described. There are several reports of keratin expression in amphibians (Ferretti et al, 1989(Ferretti et al, , 1991(Ferretti et al, , 1993Tsonis et al, 1992a,b;Ferretti, 1997, 1999;Ferretti and Ghosh, 1997;Tazawa et al, 2006). At least three antibodies recognize WE (and normal keratinocytes) (WE3, 4, and 6).…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal regulation of keratin 5 and 17 in the axolotl limb

Moriyasu

Makanae

Satoh

2012

Developmental Dynamics

View full text Add to dashboard Cite

Background: Amphibians have greater regeneration capability than higher vertebrates. They can regenerate their limbs after an amputation. As a limb is regenerated, a regeneration-specific epithelium called the apical epithelial cap (AEC) is induced. The AEC is an essential structure for limb regeneration. Despite the importance of the AEC, molecular marker genes have not been well studied at the molecular level. Results: In the present study, keratin5 (KRT5) and KRT17 were investigated in an axolotl-regenerating limb. KRT5 and KRT17 were expressed in a regenerating limb but down-regulated in a differentiating limb. KRT5 showed characteristic regulation in a regenerating blastema. KRT5 was suppressed in the basal layer of the AEC. This KRT5 suppression was correlated to the blastema differentiation and nerve presence. Simple skin wounding could also upregulate both KRT5 and KRT17 gene expression. But these genes were suppressed within a shorter time than in limb regeneration. Conclusions: The KRT5 and KRT17 gene profile can be a useful marker gene to investigate AEC in limb regeneration. Developmental Dynamics 241:1616-1624,

show abstract

Transient expression of simple epithelial keratins by mesenchymal cells of regenerating newt limb

Cited by 54 publications

References 36 publications

Mechanisms of urodele limb regeneration

Mechanisms of urodele limb regeneration

Beyond early development: Xenopus as an emerging model for the study of regenerative mechanisms

Spatiotemporal regulation of keratin 5 and 17 in the axolotl limb

Contact Info

Product

Resources

About