Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions

Sági, László; Remy, Serge; Verelst, Bert; Panis, Bart; Cammue, Bruno P. A.; Volckaert, Guido; Swennen, Rony

doi:10.1007/bf00023934

Cited by 13 publications

(4 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether this promoter remains active in mature plants will be investigated. Since the maize ubiquitin promoter is highly active in banana [ 12 , 13 , 16 , 42 ], it was expected that the 17-1 promoter would be weaker than the ubiquitin promoter as revealed by differential staining intensity of GUS assays. The results also demonstrated that sequence 17-1, originally isolated from an African plantain-type banana, is also functional in the commercial dessert banana 'Grand Naine'.…”

Section: Discussionmentioning

confidence: 99%

Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

et al. 2009

Self Cite

View full text Add to dashboard Cite

Background: Next-generation transgenic plants will require a more precise regulation of transgene expression, preferably under the control of native promoters. A genome-wide T-DNA tagging strategy was therefore performed for the identification and characterization of novel banana promoters. Embryogenic cell suspensions of a plantain-type banana were transformed with a promoterless, codon-optimized luciferase (luc + ) gene and low temperature-responsive luciferase activation was monitored in real time.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

et al. 2009

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genetic modification is an alternative approach to improving existing banana cultivars without affecting key farmer- and consumer-preferred traits. Since the development of efficient banana transformation protocols ( May et al., 1995 ; Sági et al., 1995 ), there have been numerous reports on the development of banana plants with improved nutritional content as well as resistance to a range of biotic and abiotic stresses ( Ghag and Ganapathi, 2017 ; Paul et al, 2017 ; Pua et al., 2019 ; Tripathi et al., 2019 ). In 2017, we reported the generation of several Cavendish banana lines with Foc TR4 resistance following a three-year field trial in a heavily Foc TR4-infested site in the Northern Territory, Australia ( Dale et al., 2017a ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of root-specific promoters in banana and tobacco and identification of a banana TIP2 promoter with strong root activity

et al. 2022

View full text Add to dashboard Cite

Genetic modification is one possible strategy to generate bananas (Musa spp.) with resistance to the soil-borne pathogen causing Fusarium wilt. The availability of banana root-specific promoters to target transgene expression to the sites of infection would be beneficial. We have assessed 18 promoter sequences derived from a range of plant species for their expression profiles in banana tissues to identify those with root-specific activity. Promoter sequences were isolated and fused to the β-glucuronidase (GUS) gene to assess their expression levels and tissue specificity in both banana and the model plant tobacco. Two heterologous promoters conferring high root expression levels in banana were identified, including a β‐glucosidase 1 (GLU1) promoter from maize and the RB7-type tonoplast intrinsic protein (TIP)-2 promoter from strawberry. Further, a novel Musa TIP2-2 promoter sequence was isolated and characterized which, when fused to the GUS gene, conferred very high GUS expression levels in banana roots. These promoters will expand the options for the control of gene expression in genetically modified bananas, providing a tool to develop plants with resistance not only to soil-borne diseases such as Fusarium wilt, but also for the improvement of other traits, such as nematode resistance, nutrition or abiotic stress resistance.

show abstract

“…The maximum frequency of DNA introduction as detected by an in situ TGE assay amounted to 2 to 3% of total protoplasts. Besides providing a fast method for determination of promoter strength in banana (Sági et al, 1995b), this protocol may in the future also lead to the production of transgenic banana using the plant regeneration protocol previously described (Panis et al, 1993).…”

Section: Transient Gus Expression (Tge) In Banana Protoplasts By Elecmentioning

confidence: 99%

Production of Transgenic Banana and Plantain

Sági¹,

Remy²,

Cammue³

et al. 2000

Acta Hortic.

View full text Add to dashboard Cite

Genetic transformation of banana and plantain has been performed in our laboratory by (i) particle bombardment of embryogenic cell suspensions (ECS), (ii) electroporation of protoplasts isolated from ECS, and (iii) cocultivation of meristematic tissues with Agrobacterium tumefaciens. So far, the following cultivars have been transformed successfully: Williams (AAA), Three Hand Planty (AAB), Bluggoe (ABB), Cardaba (ABB), Monthan (ABB) and Tani (BB). Transient expression of the gusA reporter gene has been observed in all these cultivars following at least one of the three transformation techniques. Transgenic plants have been regenerated after particle bombardment of Bluggoe, Three Hand Planty and Williams, representing the three economically important genomic groups in Musa. Molecular and biochemical analysis has confirmed integration and expression of the transgenes in the banana and plantain genome. At present, more than 200 independent transgenic lines, generated from more than 1000 shoots, are growing in the greenhouse. Practical applications of molecular breeding of banana and plantain to create resistance to pathogens and pests are in progress.

show abstract

Transient gene expression in transformed banana (Musa cv. Bluggoe) protoplasts and embryogenic cell suspensions

Cited by 13 publications

References 36 publications

Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress

Assessment of root-specific promoters in banana and tobacco and identification of a banana TIP2 promoter with strong root activity

Production of Transgenic Banana and Plantain

Contact Info

Product

Resources

About