Translational insights into stem cell preconditioning: From molecular mechanisms to preclinical applications

Moeinabadi-Bidgoli, Kasra; Babajani, Amirhesam; Yazdanpanah, Ghasem; Farhadihosseinabadi, Behrouz; Jamshidi, Elham; Bahrami, Soheyl; Niknejad, Hassan

doi:10.1016/j.biopha.2021.112026

Cited by 44 publications

(25 citation statements)

References 171 publications

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“…Following this fact, a strategy to improve cells’ therapeutic activities was developed named “preconditioning,” defined as culturing cells under specific conditions or with particular substances to augment their advantageous biological properties. Various methods are available to instate cell preconditioning, including hypoxic preconditioning, pharmacological agents and biologic mediators, physical preconditioning, and acidic preconditioning ( Moeinabadi-Bidgoli et al, 2021 ). However, in the context of AMPs, pro-inflammatory mediators, some growth factors, and vitamin D play more prominent roles in preconditioning approaches.…”

Section: The Role Of Preconditioning In Antimicrobial Peptides-based ...mentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Antimicrobial Peptides as Potential Anti-Neoplastic Agents: New Insight into Anticancer Mechanisms of Stem Cells and Exosomes

Moeinabadi-Bidgoli

Rezaee

Rismanchi

et al. 2022

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSCs), as adult multipotent cells, possess considerable regenerative and anti-neoplastic effects, from inducing apoptosis in the cancer cells to reducing multidrug resistance that bring them up as an appropriate alternative for cancer treatment. These cells can alter the behavior of cancer cells, the condition of the tumor microenvironment, and the activity of immune cells that result in tumor regression. It has been observed that during inflammatory conditions, a well-known feature of the tumor microenvironment, the MSCs produce and release some molecules called “antimicrobial peptides (AMPs)” with demonstrated anti-neoplastic effects. These peptides have remarkable targeted anticancer effects by attaching to the negatively charged membrane of neoplastic cells, disrupting the membrane, and interfering with intracellular pathways. Therefore, AMPs could be considered as a part of the wide-ranging anti-neoplastic effects of MSCs. This review focuses on the possible anti-neoplastic effects of MSCs-derived AMPs and their mechanisms. It also discusses preconditioning approaches and using exosomes to enhance AMP production and delivery from MSCs to cancer cells. Besides, the clinical administration of MSCs-derived AMPs, along with their challenges in clinical practice, were debated.

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Section: The Role Of Preconditioning In Antimicrobial Peptides-based ...mentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Antimicrobial Peptides as Potential Anti-Neoplastic Agents: New Insight into Anticancer Mechanisms of Stem Cells and Exosomes

Moeinabadi-Bidgoli

Rezaee

Rismanchi

et al. 2022

Front. Cell Dev. Biol.

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“… 36 Preconditioning usually implies a soft stress (hypoxia, acidic conditions, and nutrient deprivation), which allows cells to adapt to the subsequent unfriendly environment (observed in Ref. 63 ). Hypoxia stabilizes HIF-

(hypoxia-inducible factor-

), which is responsible for cell proliferation, differentiation, migration, survival, glucose adjustment, and vascularization.…”

Section: Tissue-engineered Constructs: Promises and Limitationsmentioning

confidence: 99%

Photobiomodulation in 3D tissue engineering

et al. 2022

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. Significance : The method of photobiomodulation (PBM) has been used in medicine for a long time to promote anti-inflammation and pain-resolving processes in different organs and tissues. PBM triggers numerous cellular pathways including stimulation of the mitochondrial respiratory chain, alteration of the cytoskeleton, cell death prevention, increasing proliferative activity, and directing cell differentiation. The most effective wavelengths for PBM are found within the optical window (750 to 1100 nm), in which light can permeate tissues and other water-containing structures to depths of up to a few cm. PBM already finds its applications in the developing fields of tissue engineering and regenerative medicine. However, the diversity of three-dimensional (3D) systems, irradiation sources, and protocols intricate the PBM applications. Aim: We aim to discuss the PBM and 3D tissue engineered constructs to define the fields of interest for PBM applications in tissue engineering. Approach : First, we provide a brief overview of PBM and the timeline of its development. Then, we discuss the optical properties of 3D cultivation systems and important points of light dosimetry. Finally, we analyze the cellular pathways induced by PBM and outcomes observed in various 3D tissue-engineered constructs: hydrogels, scaffolds, spheroids, cell sheets, bioprinted structures, and organoids. Results: Our summarized results demonstrate the great potential of PBM in the stimulation of the cell survival and viability in 3D conditions. The strategies to achieve different cell physiology states with particular PBM parameters are outlined. Conclusions: PBM has already proved itself as a convenient and effective tool to prevent drastic cellular events in the stress conditions. Because of the poor viability of cells in scaffolds and the convenience of PBM devices, 3D tissue engineering is a perspective field for PBM applications.

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“…With advances in cell therapy, stem cell repair of IVDD appears to be a good strategy. Based on the tissue engineering, stem cells have been widely used in regenerative medicine owing to their advantages of self-proliferation and directed differentiation at degeneration sites (Moeinabadi-Bidgoli et al, 2021). Mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), and adipose stem cells can differentiate into NP cells (NPCs) and synthesize new ECM for IVDD regeneration (Baldia et al, 2021;Ekram et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Application of mesenchymal stem cell-derived exosomes from different sources in intervertebral disc degeneration

Xia

Yang²,

Hou³

et al. 2022

Front. Bioeng. Biotechnol.

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Intervertebral disc degeneration (IVDD) is a main cause of lower back pain, leading to psychological and economic burdens to patients. Physical therapy only delays pain in patients but cannot eliminate the cause of IVDD. Surgery is required when the patient cannot tolerate pain or has severe neurological symptoms. Although surgical resection of IVD or decompression of the laminae eliminates the diseased segment, it damages adjacent normal IVD. There is also a risk of re-protrusion after IVD removal. Cell therapy has played a crucial role in the development of regenerative medicine. Cell transplantation promotes regeneration of degenerative tissue. However, owing to the lack of vascular structure in IVD, sufficient nutrients cannot be provided for transplanted mesenchymal stem cells (MSCs). In addition, dead cells release harmful substances that aggravate IVDD. Extracellular vesicles (EVs) have been extensively studied as an emerging therapeutic approach. EVs generated by paracrine MSCs retain the potential of MSCs and serve as carriers to deliver their contents to target cells to regulate target cell activity. Owing to their double-layered membrane structure, EVs have a low immunogenicity and no immune rejection. Therefore, EVs are considered an emerging therapeutic modality in IVDD. However, they are limited by mass production and low loading rates. In this review, the structure of IVD and advantages of EVs are introduced, and the application of MSC-EVs in IVDD is discussed. The current limitations of EVs and future applications are described.

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Translational insights into stem cell preconditioning: From molecular mechanisms to preclinical applications

Cited by 44 publications

References 171 publications

Mesenchymal Stem Cell-Derived Antimicrobial Peptides as Potential Anti-Neoplastic Agents: New Insight into Anticancer Mechanisms of Stem Cells and Exosomes

Mesenchymal Stem Cell-Derived Antimicrobial Peptides as Potential Anti-Neoplastic Agents: New Insight into Anticancer Mechanisms of Stem Cells and Exosomes

Photobiomodulation in 3D tissue engineering

Application of mesenchymal stem cell-derived exosomes from different sources in intervertebral disc degeneration

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