Translational Opportunities in Stem Cell–based Endodontic Therapy: Where Are We and What Are We Missing?

Peters, Ove A.

doi:10.1016/j.joen.2014.01.017

Cited by 14 publications

(11 citation statements)

References 51 publications

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“…Regenerative endodontics represents a new treatment modality that relies on the intracanal delivery of stem cells and focuses on re-establishment of pulp vitality and continued root development (Chrepa et al, 2015;Peters, 2014). Numerous attempts, using human DMSCs (hDPSCs), have been made in a variety of animal models in order to achieve complete pulp regeneration.…”

Section: Regeneration Of Pulp-dentin Complexmentioning

confidence: 99%

“…Numerous attempts, using human DMSCs (hDPSCs), have been made in a variety of animal models in order to achieve complete pulp regeneration. Proper regeneration requires re-vascularisation and re-innervation of the pulp and allows new dentin formation (Peters, 2014). The very first experimental study using hDMSCs showed that these cells can differentiate into odontoblasts, which form dentin-like structures when transplanted together with HA/TCP ceramic powder in immunocompromised mice ex vivo (Fig.…”

Section: Regeneration Of Pulp-dentin Complexmentioning

confidence: 99%

“…However, the current status of stem cell-based endodontic therapy is still characterised by an empirical approach (Peters, 2014).…”

Section: Regeneration Of Pulp-dentin Complexmentioning

confidence: 99%

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Stem cell-based approaches in dentistry

Mitsiadis

Orsini

Jiménez-Rojo

2015

eCM

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Repair of dental pulp and periodontal lesions remains a major clinical challenge. Classical dental treatments require the use of specialised tissue-adapted materials with still questionable efficacy and durability. Stem cell-based therapeutic approaches could offer an attractive alternative in dentistry since they can promise physiologically improved structural and functional outcomes. These therapies necessitate a sufficient number of specific stem cell populations for implantation. Dental mesenchymal stem cells can be easily isolated and are amenable to in vitro expansion while retaining their stemness. In vivo studies realised in small and large animals have evidenced the potential of dental mesenchymal stem cells to promote pulp and periodontal regeneration, but have also underlined new important challenges. The homogeneity of stem cell populations and their quality control, the delivery method, the quality of the regenerated dental tissues and their integration to the host tissue are some of the key challenges. The use of bioactive scaffolds that can elicit effective tissue repair response, through activation and mobilisation of endogenous stem cell populations, constitutes another emerging therapeutic strategy. Finally, the use of stem cells and induced pluripotent cells for the regeneration of entire teeth represents a novel promising alternative to dental implant treatment after tooth loss. In this mini-review, we present the currently applied techniques in restorative dentistry and the various attempts that are made to bridge gaps in knowledge regarding treatment strategies by translating basic stem cell research into the dental practice.

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Section: Regeneration Of Pulp-dentin Complexmentioning

confidence: 99%

Section: Regeneration Of Pulp-dentin Complexmentioning

confidence: 99%

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Stem cell-based approaches in dentistry

Mitsiadis

Orsini

Jiménez-Rojo

2015

eCM

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“…). To establish reliable protocols for RET, the influence of each treatment step, including irrigation, dentine treatment and scaffold selection, on the expected outcome, must be evaluated (Peters ). The clinical success of RET on continued root development has been described previously (Murray et al .…”

Section: Discussionmentioning

confidence: 99%

“…Regenerative endodontic treatment (RET) is a biological procedure designed to replace damaged structures (Murray et al 2007). To establish reliable protocols for RET, the influence of each treatment step, including irrigation, dentine treatment and scaffold selection, on the expected outcome, must be evaluated (Peters 2014). The clinical success of RET on continued root development has been described previously (Murray et al 2007, Jung et al 2008, Raju et al 2014, although no studies have attempted to analyse the longitudinal immune response to tissue engineering, which is not possible to analyse in humans with the currently available methods.…”

Section: Discussionmentioning

confidence: 99%

Effect of ethylenediaminetetraacetic acid irrigation on immune‐inflammatory response in teeth submitted to regenerative endodontic therapy

et al. 2019

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Aim To analyse longitudinally the immune‐inflammatory response in teeth of mice that underwent a regenerative protocol with or without the use of ethylenediaminetetraacetic acid (EDTA) to irrigate the root canal system. Methodology First maxillary molars of mice were devitalized using size 10 and 15 files. Teeth were divided into the following groups: Empty – the canals were left empty; Blood Clot (BC) – the canals were filled with a blood clot; and EDTA + Blood – the canals were irrigated with 0.06 mL of 17% EDTA for 1 min and filled with a blood clot. Access cavities were restored with Coltosol®. Animals were sacrificed at 7, 14 or 21 days after the operative procedures, and teeth were collected. RNA was extracted, mRNA expression of the cytokines IGF, NGF, IL‐1α, IL‐10, TGF and VEGF was assessed using real‐time PCR, and the anova Kruskal–Wallis test was used. Results IL‐1 mRNA expression was significantly higher in the EDTA + BC group than in the Empty and BC groups at the 7th and 14th days of evaluation (P < 0.05). IL‐10 mRNA expression was similar across the three groups at all time periods. TGF‐β mRNA expression in the EDTA + BC group was significantly higher on the 7th and 21st days than on the 14th (P < 0.05); at day 21, TGF‐β mRNA expression was similar between the BC and EDTA + BC groups but significantly higher than in the Empty group (P < 0.05). IGF mRNA expression was significantly higher in the EDTA + BC group than in the other groups at all time periods. VEGF mRNA expression remained unchanged throughout the experimental period in all groups (P > 0.05). NGF mRNA expression was similar amongst all groups at the 7th and 21st days (P > 0.05). At the 14th day, however, there was a significant increase in NGF mRNA expression in the EDTA + Blood group (P < 0.05) when compared with the expression in the other groups. Conclusion EDTA promoted increased expression of factors that have the potential to improve the outcome of regenerative endodontic treatment.

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Recent Advances in Stem Cells for Dental Tissue Engineering

Nizami

Nishina

2021

Engineering Materials for Stem Cell Regeneration

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Translational Opportunities in Stem Cell–based Endodontic Therapy: Where Are We and What Are We Missing?

Cited by 14 publications

References 51 publications

Stem cell-based approaches in dentistry

Stem cell-based approaches in dentistry

Effect of ethylenediaminetetraacetic acid irrigation on immune‐inflammatory response in teeth submitted to regenerative endodontic therapy

Recent Advances in Stem Cells for Dental Tissue Engineering

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