Translational proteomics and phosphoproteomics: Tissue to extracellular vesicles

Wu, Xiaofeng; Iliuk, Anton; Tao, W. Andy

doi:10.1016/bs.acc.2022.09.003

Cited by 1 publication

(1 citation statement)

References 208 publications

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“…Proteomic analysis is considered as a promising strategy to get a global view of complex biological processes, taking advantage of high-throughput profiling ( Cui et al, 2022 ) and extensive dynamic ranges ( Canterbury et al, 2008 ). Recently, liquid chromatography-mass spectrometry (LC–MS)-based proteomics have been utilized for investigating biomarkers in various biological samples, such as extracellular vesicles ( Wu et al, 2023 ), biological tissues ( Li et al, 2022 ), serum ( Zhang et al, 2022 ), and paraffin-embedded tissues ( Cai et al, 2022 ), indicating wide clinical applications regarding cancers and diseases. Rapidly developed proteomic technology provides a more precise and more comprehensive insight into virus or bacterial infections ( Grossegesse et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Temporal proteomic profiling reveals functional pathways in vaccinia virus-induced cell migration

Liu

Chen

et al. 2023

Front. Microbiol.

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IntroductionViral diseases have always been intricate and persistent issues throughout the world and there is a lack of holistic discoveries regarding the molecular dysregulations of virus-host interactions. The temporal proteomics strategy can identify various differentially expressed proteins and offer collaborated interaction networks under pathological conditions.MethodHerein, temporal proteomics at various hours post infection of Vero cells were launched to uncover molecular alternations during vaccinia virus (VACV)-induced cell migration. Different stages of infection were included to differentiate gene ontologies and critical pathways at specific time points of infection via bioinformatics.ResultsBioinformatic results showed functional and distinct ontologies and pathways at different stages of virus infection. The enrichment of interaction networks and pathways verified the significances of the regulation of actin cytoskeleton and lamellipodia during VACV-induced fast cell motility.DiscussionThe current results offer a systematic proteomic profiling of molecular dysregulations at different stages of VACV infection and potential biomedical targets for treating viral diseases.

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